جستجوی مقالات مرتبط با کلیدواژه « sulfonamides » در نشریات گروه « زیست شناسی »

Antimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord. In this study, antibiotic resistance 90 isolates of Klebsiella pneumoniae isolated from clinical cases of Shahrekord,disk diffusion method was performed. In order to investigate resistance to sulfonamides of the antibiotic cotrimoxazole was used. Then using specific primers was performed tracing genes sul1, sul2 and sul3 and intI. After PCR reaction of 33 isolates resistant to trimethoprim-sulfamethoxazole sul1 gene in 15 isolates (45/45%), sul2 gene in 20 isolates (60/60%), gene Sul3 in 2 isolates (6/06%) and the gene intI 27 isolates was found. The statistical analysis between genes sul1and IntI significant relationship was observed. The results of this study show that is a strong correlation between carry integrons and increased resistance to a number of different classes of antibiotics.

Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to form cross-sectional in 2013. Samples was prepared as sterile and in terms of urine tests, cultures and was studied. Investigation antimicrobial susceptibility was performed by disk diffusion method. As well as, for tracing Sul gene PCR reaction was performed in the presence of specific primers and the results was analyzed. In this study of 130 E. coli isolates studied 67 isolates (53/51%) resistance to co-trimoxazol was observed. The frequency of genes sul1, sul2 and sul3 was respectively 20/89%, 55/22% and 4/47%. In statistical analysis with chi-square test between to resistance sulfonamides and sul genes significant correlation was observed. The results showed that E. coli isolates are high resistant to sulfonamides that may be the indiscriminate use of these antibiotics.