جستجوی مقالات مرتبط با کلیدواژه "tuberculosis" در نشریات گروه "زیست شناسی"

Molecular docking of major virulence factors of bacteria by bioactive compounds has obtained huge attention in molecular docking studies. Theaflavin-3-gallate related to the polyphenolic bioflavonoid class isolated from Camellia sinensis leaves has demonstrated anti-inflammatory, anticancer, antibacterial, and antiviral activities as important therapeutic properties. There are several main virulence factors for Mycobacterium tuberculosis, the causative agent of tuberculosis. In the present study, protein kinase A, ESX-1 system, and ESX-1 secreted protein B (EspB) were opted to survey molecular docking interaction upon theaflavin-3-gallate. This investigation revealed that different docking scores resulted from CB-Dock2 and AutoDock Vina. CB-dock2 results demonstrated a higher affinity of theaflavin-3-gallate ligand with protein kinase A receptor. In contrast, based on the results of AutoDock Vina, there was the highest binding affinity for the ESX-1 system receptor with a binding energy of -10.1 kcal/mol. Moreover, the STRING database demonstrated higher scores of 0.998 and 0.995 for pstp and EspL for protein kinase A and EspB, respectively.

tRNA modifications play a significant role in the structural stability as well as translational fidelity in all organisms from bacteria to humans. They also play a major role in bacterial physiology by regulating translation in response to various environmental stresses. Modifications coming at the anticodon-stem loop (ASL) are particularly important as they stabilize codon-anticodon interactions, ensuring accuracy and speed in decoding mRNAs  Addition of isopentenyl group (i6A) at A37 position by tRNA isopentenyltransferase (MiaA) is a well conserved modification from bacteria to human. We studied M. tuberculosis MiaA from strain H37Rv and identified the target tRNAs for this modification based on the A36A37A38 motif. i6A modification of target tRNAs tRNALeuCAA, tRNAPheGAA, tRNATrpCCA and tRNASerCGA were further confirmed by isopentenyltransferase assay providing the substrate DMAPP and recombinant MiaA enzyme.

Diabetes is one of the most common non-contagious diseases in the world. This disease is the fourth or fifth cause of death in most developed countries. The relationship between tuberculosis and diabetes had been introduced years ago and diabetes is considered a threatening factor in tuberculosis in the research history. Not only tuberculosis is prevalent among diabetic persons, but also diabetes can affect the appearance of imaging of tuberculosis. This is a kind of analytic study, a case-control study, which was carried out between the years 2014 and 2015 in Zabol City. In this study, the radiographic findings from the patients suffering from pulmonary tuberculosis in diabetic patients and not diabetic patients are compared. These radiographic were handed to a radiologist and the radiologic findings of these graphs from the anatomic location (upper and lower half of the right and left bellows), Presence or absence of cavities, nodules, density and pleural involvement were recorded and compared with the diabetic and non-diabetic group. After gathering of the data using SPSS software, descriptive statistics were presented in the form of (frequency, percent) graphs and a chi-square test (p<0.05) was used to analyze and comparing of the results of diabetic and un-diabetic patients. The population of the study consisted of Chest radiographs for 124 TB patients which 61 (49.19 %) were suffering from diabetics. In this study, 45 (71.43%) non-diabetic and 42 (65.85%) diabetic patients were women (p=0.7). There were 12 diabetic TB patients (19.67%) and 3 non-diabetic- TB patients (4.76%) with the consolidation of middle part of left lung (p=0.01) and 8 (13.11%) diabetic TB and 1 (1.59%) non-diabetic TB with reticulonodular infiltration of lower part of left lung (p=0.02). There was no significant difference in the rest of the radiographic results. The findings of this study indicate that reticulonodular infiltration and consolidation of lower &middle parts of the lung in TB diabetic patients is more than in TB non-diabetic patients and diabetes can affect the findings of pulmonary tuberculosis radiography.

The DNA polymorphisms found in clinical strains of Mycobacterium tuberculosis drive altered physiology, virulence, and pathogenesis in them. This study aimed to investigate the association between IL23R 1142 G/A (Arg381Gln) and GM-CSF 3928 C/T (Ile117Thr) gene polymorphisms with the incidence rate of tuberculosis in the population of Sistan. This study was based on the descriptive and applied type. All patients with active pulmonary tuberculosis were referred to the tuberculosis center of Zabol city for one year, with an equal number of healthy people adapted to the patients examined in terms of age. After data collecting to compare the frequency of polymorphisms, the chi-square test and OR index were used using SPSS software version 16. We have found that the IL23R reduced-function allele 1142A and genotypes CC and TC were overrepresented, especially in the Pad subgroup compared with the control group (44% versus 42%, 21% versus 22%, and 44% versus 39%, respectively. Increased risks of TB with minimal/moderate lung involvement, respectively. Our results demonstrate that the reduced-function polymorphism 1142G ¡ A encoded by IL23R influences the outcome of disease severity of active pulmonary TB in ZABOL patients. The genotypic and allelic frequency of IL23R 1142 G/A, and GM-CSF 3928 C/T (Ile117Thr) polymorphism in patients with tuberculosis was significantly different from the control group and this polymorphism was associated with the incidence of tuberculosis in the population of Sistan.

Tuberculosis is a vagarious infectious disease that generally affects the lungs. Accordingly, in some cases, it can also affect the liver and kidney. Host genetic may affect tuberculosis caused by bacillus Mycobacterium tuberculosis. The main risk factors for the disease are a weakened immune system because of diabetes, some cancers, HIV/AIDS, severe kidney disease, cancer treatment, and malnutrition. Il-33 is involved in the activation of eosinophils, mast cells, basophils, and natural killer cells and the maturation of T helper type2 cells. The developments of CD4 (+) TH1 and CD8 (+) T cell responses are involved in protection against TB, IL-33 promotes the development of these cells. The purpose of the present research was to investigate the association between Mycobacterium tuberculosis infection and IL-33 gene polymorphisms (rs1157505C/G and rs11792633C/T) with tuberculosis in the cases and controls from the area of high tuberculosis prevalence in Iran. In this study, 100 patients with tuberculosis disease and 91 healthy controls were included. Polymorphisms of the IL-33 gene were genotyped using T-ARMS-PCR. The analysis of the haplotype combinations among IL-33 polymorphisms demonstrated that the magnitude of the association was higher for the combined CC/CT genotypes. The CT genotype related to C/T polymorphism of the IL-33 gene increased the risk of tuberculosis. The combined CG+GG genotypes related to C/G polymorphism of the IL-33 gene also increased the risk of tuberculosis, but the difference was not statistically significant. In conclusion, IL-33 gene polymorphisms may be considered as important contributors to tuberculosis in Iran.