جستجوی مقالات مرتبط با کلیدواژه « سرطان ریه » در نشریات گروه « زیست شناسی »

Lung cancer is a type of malignant lung tumor, which is the most important cause of death caused by cancer in humans. GLUT1 is an important transporter of glucose to cells, and its over expression is involved in increasing the metabolism of cancer cells. Investigating changes in GLUT1 gene expression in non-small cell cancer tissue samples. Number of 30 tissue samples of non-small cell lung cancer and 30 tissue samples adjacent to the tumor were obtained from patients referred to Masih Deneshvari Hospital after obtaining consent. After sampling, RNA isolation and cDNA production, GLUT1 and GAPDH mRNA level was measured by Real-Time PCR technique. Statistical analysis of gene expression data was done with t test. In 21 samples (70%), GLUT1 mRNA level in non-small cell lung cancer tissue was increased compared to healthy tissue. In addition, the level of mRNA in tumor tissue increased by 3.4 times compared to normal tissue (P = 0.013). The increase in GLUT1 mRNA level in non-small cell lung carcinoma cells can be a sign of changing the metabolic program of these cells to aerobic glycolysis, which results in tumor expansion and malignancy.

Lung cancer is one of the most common types of cancer in the world and Iran. Herbs are a source of a variety of antioxidant compounds that can be used to produce anti-cancer drugs. In the present study, the effect of cytotoxicity of essential oil and hydroalcoholic extract of Juniperus excelsa on A549 lung cancer cells was investigated. The branches of male rootstocks of Aras plant were collected from Chehel Cheshmeh in Firoozkooh and Sirachal in Karaj and the essential oil and extract were prepared by Clevenger and soaking methods, respectively. The degree of cytotoxicity, different concentrations of essential oil and extract of the two regions, on A549 cells in three times of 24, 48, and 72 hours Was evaluated using MTT method. Flow cytometry was used to evaluate cell death and cell cycle in cells treated for 72 hours with 10 µg/ml of essential oil and extract of two regions. The results of cytotoxicity showed that the lethal power of extracts was significantly higher than essential oils, but there was no significant difference between different regions (P <0.01). Also, a further decrease in the number of viable cells and an increase in cell entry into the Sub G1 phase, resulting in an increase in apoptosis treated with male root extract, was proven. Thus, the extract of the branches of this plant has anti-cancer properties by stopping the cell cycle in the G2 / M stage.

 Lung cancer is the most killer cancer in both men and female, as the lung cancer mortality rate is higher than death by the next two breast and prostate cancers. Lung cancer treatment compared to breast cancer is almost unsatisfied, since the most of lung cancer cases are diagnosed at a stage of the disease where metastasis has occurred. The cause of late diagnosis in lung cancer is the similarity of signs and symptoms of cancer with pulmonary symptoms and lack of confidence approaches to identify it at early stage. Nowadays, much attempt is paid to biomarkers such micro RNAs, exosomes, and tetraspanins that can give us information about intracellular events. Microfluidic as science is the study of fluid behavior, and as technology is the construction and use of the specific properties in these dimension which is evolving and entering various fields, from chemical synthesis and biological analysis to optics and even information technology. In this work, we designed and fabricated via UV lithography a microfluidic chip for lung cancer cell derived exosomes capture followed by detection by CD63/CD151-FITC antibodies. The capture exosomes then extracted from microfluidic chip and investigated by Atomic Force Microscopy, AFM, and Dynamic Light Scattering DLS. The average size of exosomes was measured 19-37 nm by DLS and 35-37 nm by AFM that are similar to each other.

Circulating miRNAs have also been proposed as novel biomarkers for early diagnosis and even prognosis of cancer. Due to the non-invasive access, in addition to before metastase early detection, the use of these molecules can reduced injuries of patients. In this study, the biosensor was designed to isolate circulating mi RNAs by microfluidic system. The design was based on the isolation of two miRNAs (miR-21 and miR-486), which have been described in papers as miRNAs with potential to diagnosis of lung cancer.In this system, miRNA isolation is performed using a capture probe (single-strand DNA), immobilized by GPTMS linker on PDMS surface. By attaching of microRNA to this probe, the second probe that was biotinilated DNA complement to upper portion of microRNA, attaches to it and creates a sandwich structure. Eventually, trapped microRNA between two probes was detected by FITC- streptavidin. miRNA has been visualized under IX81 Olympus florescent microscope.

Lung cancer is the most common malignancy with the highest mortality worldwide. LncRNAs play complex roles in the initiation and progression of lung cancer, thereby affecting the prognosis of patients. In this study, the expression changes of CCAT1, CCHE1, ANRIL, BANCR, and PICART1 lncRNAs in tumor tissues compared to normal tissues adjacent to the tumor were investigated and their potential as biomarkers for the diagnosis and prognosis of lung cancer was evaluated. In order to conduct this case-control study, 30 samples of lung cancer tissue and 30 samples of adjacent non-cancerous tissues (ANCTs) were collected and after total RNA extraction and cDNA synthesis, the expression levels of target lncRNAs and β2M reference gene was measured using SYBR Green real-time PCR assay. The statistical analysis of Real-time PCR data showed no significant difference between the expression of these lncRNAs in tumor tissue and normal tissue adjacent to the tumor (P> 0.05). Furthermore, no significant association was observed between the expression level of these lncRNAs and any of the demographic and pathological characteristics of patients (P> 0.05). Based on the findings of this study, it can be concluded that these lncRNAs play no role in lung cancer in Iranian patients. However, to confirm our results, it is necessary to evaluate the expression of these lncRNAs in a larger number of samples..

Studies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study, the cytotoxic effects of testosterone on Hek293 and A549 cells were assayed using MTT method. Real time PCR was used to evaluate the expression levels of Bax and Bcl2 genes in A549 cells exposed to IC50 dose of testosterone. The data were statistically analyzed between groups using one-way ANOVA. Exposure of Hek293 and A549 cells to higher dose of testosterone (1000 microg/ml) of testosterone resulted in significant decrease in cell viability (P<0.001). IC50 dose of testosterone significantly decreased the anti-apoptotic Bcl-2 gene expression level (P<0.001) in A549 cells, however, did not significantly change the expression level of apoptotic Bax gene. The cytotoxic concentration of testosterone induces apoptosis in lung cancer cells by its inhibitory effect on anti-apoptotic Bcl-2 gene expression level. Accordingly, appropriate dose of testosterone has anti-cancer effects against lung cancer cells.

Lung cancer is one of the major causes of cancer deaths. The activation of fgf signals, including fgf-9, is involved in the pathogenesis of several cancers, including lung cancer. Also, fgf-9 may be an indicator for prognosis and is associated with survival rates in patients with NSCLC. Therefore, in this study, the rate of fgf-9 gene expression in the serum patients with lung cancer was investigated. Analysis

In this study, 50 serum samples of healthy subjects and 50 serum samples of NSCLC patients were collected from patients referring to Masih Daneshvari Hospital in Tehran and were collected by questionnaires, individual and clinical data of the subjects. Then, Plasma isolation, RNA extraction, cDNA synthesis, primer design were performed and the rate of changes of fgf-9 expression in the serum of healthy and lung cancer patients was evaluated using Real Time PCR. REST software was used to analyze the results.

In this study, the expression of fgf-9 gene was not significantly different in the serum of patients with the first to third stages of metastasis but in the serum of people with stage IV metastasis the level of expression of fgf-9 gene had a significant reduction of 4.46 (p < 0.05) times than normal samples.

According to the results of this study, if these results are confirmed in more samples, the level of the fgf-9 gene expression in the serum of individuals can probably be used in the future to predict the stage of metastasis of lung cancer

Lung cancer is one of the most common cancers in both men and women and it is growing worldwide. Previous studies have shown that using Zinc Oxide nanoparticles (ZnO-NP) can be a promising agent in cancer treatment, because of their unique properties. The purpose of current study is to investigate the effect of ZnO-NP on oxidative stress in A549 human non-small lung cancer cells. A549 cells with were treated with different concentrations of Zinc Oxide nanoparticles. MTT assay and Trypan blue staining were performed to measure IC50 concentration and cell viability on days 1, 3, 5 and 7. Also, to investigate the apoptotic effects of IC50 concentration of these nanoparticles, Giemsa and AO/EB staining methods and anti-oxidant enzymes superoxide dismutase and catalase activity measurements were used. The results showed that ZnO-NP reduced the A549 cells viabilities in a dose and time dependent manner, and the obtained IC50 concentration was 5mg/mL. The results of stainings indicated an increase in percentage of apoptotic cells in treatment groups. Furthermore, enzyme measurement results showed that the activity of both enzymes in treatment groups was significantly increased in comparison to the control group. The results of this study demonstrated that ZnO-NP have the ability to induce cell death in lung cancer cells and we hope that with organizing this nanoparticle as a new medical compound, it will be used in cancer treatment.

Aim: In current study, we aimed to reduce Cdc42 gene expression in lung carcinoma related cells, Calu-6, and assayed its effect on cell proliferation.
Material and To reduce the expression of Cdc42 gene shRNA system was used and lentiviral system was selected to deliver Cdc42 specific shRNA to Calu-6 cells. Recombinant lentiviruses produced by co-transfection of pMD2G, psPAX2 and p-GFP-C-shLenti plasmids into 293T cells using lipofectamin. Efficiency of transfection and transduction assessed by florescent microscopy. Viability of cells treated by recombinant lentiviruses assessed by MTT assay.
florescent microscopy showed 80% transfection of 293T cells and high rate of Calu-6 cells transduction. MTT assay results revealed that viability of transduced Calu-6 cells reached to %58 and %40 in compare to control and negative control cells, respectively.
recombinant lentiviruses properly transfer Cdc42-shRNA into Calu-6 cells, leading to reduction of cell proliferation. Silencing of Cdc42 gene expression using lentiviruses is persist and long-term effect which can be under attention for gene therapy of lung cancer.

Inroduction & Lung cancer is the most common malignancy and the leading cause of cancer related mortality among women and men worldwide, in such a way that the 5-year survival of patients is about 15%. According to the invasive role of CXCL12, challenges in various studies about expression and non-expression of CXCL12 and the presence of metastasis in early stages of lung adenocarcinoma, the A549 cell line is selected in order to investigate CXCL12 gene expression in it.
Material and In this study A549 cell line was cultured in a DMEM medium contain 10% fetal serum bovine and 1% penicillin- streptomycin. RNA extraction was performed from the mentioned cell line and after qualitative and quantitative evaluation of extracted total RNA, cDNA was synthesized. Then CXCL12 expression was measured using Real time PCR.
CXCL12 low expression in lung adenocarcinoma A549 Cell line shows that it can be considered as a potential marker for predictive and therapeutic purposes.
CXCLL12 has a low expression in A549 cell line.

One of the common causes of cancer-related mortalities worldwide is lung cancer and more than 1 million people annually die from it. Lung cancers are divided into Non-small cell lung cancer (NSCLC) and small cell lung carcinomas (SCLCs) which are different in several characteristics like biological behavior, response to therapy and genetic alterations.Resistance to conventional chemotherapy, especially in NSCLC patients is a major challenge in the treatment process. Therefore, development of novel therapeutic strategies is essential for improvement of survival rate. Combination of different therapeutic methods is an approach which can maximize efficiency of the treatment.Small interfering RNA (siRNA) has been reported to have a huge potential for the treatment or prevention of various lung diseases. Once the RNA molecules have successfully entered the target cells, they could inhibit the expression of specific gene sequence through RNA interference (RNAi) mechanism and generate therapeutic effects. The biggest obstacle to translating siRNA therapy from the laboratories into the clinics is delivery. An ideal delivery system should protect the siRNA from enzymatic degradation, facilitate cellular uptake and promote endosomal escape inside the cells, with negligible toxicity.Very limited work has been done on the formulation of siRNA for inhalation which is believed to be the direction for future development. This review article introduces lung cancer and siRNA as a new treatment, and also studies the challenges of siRNA delivery to lung cells by non-viral carriers. Furthermore, it surveyed some nano carriers for pulmonary siRNA delivery with lipid, polymer and peptide basic.

Cytotoxic effect of extract leaves of T. kotschyanus Boiss. & Hohen on Human Lang Cell. T. kotschyanus BOISS. & Hohen was used in traditional medicine such as anti-inflammation, antispasmodic and gastrointestinal aim of this study was to survey the cytotoxic effects the of ethanol of leave of thymus kotschyanus on A-549 cell line. T. kotschyanus was collected on spring 2014 from Selseleh regions of Lorestan province leaves plant for 3 days at room temperature. Dried herd powder was and extract ethanol. After preparing extract 25, 50, 100, 20, 500 μg/ml concentration for extract leveas T. kotschyanus on the cells were evaluated for 7 2 hours. Cytotoxic effects of T. kotschyanus extract leveas T. kotschyanus against cancer cell was measured by using SPSS. Results shows that, the extracts leaves ethanol T. kotschyanus has cytotoxic effects on A-549 cell lien. The finding suggest that extracts ethanol leaves T. kotschyanus to existence phenolic compounds, especially flavonoids has an inhibitory effect on the A549 cell line.