جستجوی مقالات مرتبط با کلیدواژه « Albumin » در نشریات گروه « زیست شناسی »

One of the important issues for quality control of biological products is purity. In most cases, electrophoresis is used to determine the purity of the proteins and the quantity of related band is determined by densitometry and respective software. In order to check the purity of hyperimmune plasma and antivenom bulks, we have evaluated the albumin content of these products. Sixty samples were divided into two groups: the hyperimmune plasma group that containing thirty bulks of monovalent and polyvalent anti-snake, polyvalent anti-scorpion and anti-diphtheria toxin plasma, and plasma-derived antivenoms group, comprising thirty bulks sera obtained from the purification of relevant plasma bulks. The albumin purification from horse normal serum was performed through heat shock strategy and used as reference for the quantification of albumin content. After electrophoresis of samples, the gel images were analyzed using Image J software and the amount of protein in relevant band was determined by calculating the curve area and comparison with reference albumin. The results revealed significant changes on albumin level in hyperimmune plasma samples. Two out of 30 plasma samples had lower and sixteen samples had a higher albumin than normal levels. Using the Image J, we could not detect any residue of albumin in antivenom sera. The results demonstrated effectiveness of purification protocol for removal of albumin during antivenom production process. However, development of a quantitative evaluation method along with the semi-quantitative method used in this study can help the accuracy of these results.

This study aimed to investigate the effect of highly protein diet on some haematological and serum biochemical parameters in female and male Japanese quail (Coturnix japonica) at different age stages. Three different age stages of birds (0-30, 30-44 and >44 days) were included. After measuring the weight of birds, they were slaughtered and a blood samples were taken. Based on the results, there was an increase in the general protein and albumin in females and males at the age 3 months. The globin was higher in males. Reagrding the ratio of phosphorus and calcium, they were higher in females than males and alkaline phosphate and hemoglobin were elevated in males at 3 months of age, and in females was raised at 1st month. An increase from the normal level of total protein, albumin, calcium, hemoglobin and, alkaline phosphatase and a decrease of phosphorous and globin were observed by ageing. Many of the parameters were different significantly between sexes.

This study was designed to evaluate the levels of some biomarkers, including Galectin-3, Periostin, and Interleukin-33 in male albino rats induced as asthma disease model using albumin extracted from the egg. In addition, the histological and physiological indices were investigated to reveal of the protective role of fish oil (omega -3) (2-4mg/kg of body weight) against asthma induced by albumin for four weeks. These biomarkers are new diagnostic biomarkers for asthma-induced disease by albumen extracted from the egg and aluminum hydroxide. For this purpose, forty male albino rats were treated during 2020 to December 2020. The experimental animals are divided into four groups included: (1) treated group: ten animals for one month administrated with 2mg/kg omega-3, (2) 10 animals for one month administrated with 4mg/kg omega-3, (3) asthma induced group with 10 animals, and control group 10 animals. The results showed significant increases in serum concentrations of Galectin-3, Periostin, and Intrleukin-33 in induced asthma albino rats group (P<0.05), while there was a significant decrease in serum concentrations of Galectin-3, Periostin, and Interleukin-33 in the groups which treated with omega-3 fish oil as compared with induced asthma albino rats group (P<0.05). The histological sections of albino rats’ lungs revealed asthma induction in lung tissue which represented by degeneration, necrosis and high amount of the mucous production in bronchioles as well as infiltration of inflammatory cells around bronchioles while effects of albumin in bronchi and trachea sections showed sloughing, degeneration and necrosis around the epithelium of bronchioles. Based on the results, fish oil omega-3 has improved most histological and physiological deterioration.

In vitro fertilization (IVF) is offered as a treatment for infertility in women.. The Cell free DNA in plasma/follicles were suggested as a biomarker for embryo quality inIVF. Higher the levels of Cell-free DNA indicate poorer embryo quality in the follicular fluidduring IVF. Therefore, this study was designed to examine the cell-free DNA (cfDNA) inplasma and Fluid-follicle of women undergoing IVF-embryo transfer. 

The studyexamined 100 women undergoing IVF treatment in an IVF unit in Yas Hospital of TehranUniversity of Medical Sciences in Tehran-Iran. Cell-free DNA was extracted from plasmaand follicular fluid of patients using the NucleoSpin® Extraction Kit. The total cfDNA wasexamined by qPCR for ALBUMIN and GAPDH genes. 

we did not find astatistically significant association among the variables such as CT-plasma, CT-follicularfluid, delta-CT and average of these data between two groups (including patients who had asuccessful IVF cycle and those who had a failed IVF cycle). 

Plasma andfollicular fluid cfDNA may reflect the presence of factors which interfere with embryoimplantation. Further research is required to determine the usefulness of cfDNA as abiomarker of IVF outcome and to examine the underlying pathologies as potential sourcesfor increased plasma/follicular fluid cfDNA concentrations.

There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. There are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consuming method and it needs machinery and plant. In the present study the fractionation of human plasma by polyethylene glycol was investigated. The purpose of this study was to investigate the possibility of the fractionation of human plasma by polyethylene glycol. Human plasma fractionation was carried out by using polyethylene glycol at different concentrations from five to twenty percent, and it was followed by centrifugation. After each step of addition of polyethylene glycol the supernatant was removed for further fractionation by addition of higher concentration of polyethylene glycol. Suitable intermediate sources for protein purification were obtained by fractionation of human plasma by polyethylene glycol. Fibrinogen in fraction 5%, IgG and IgM in fraction 10%, IgA in fraction 20%, and finally albumin and α1-Antitrypsin in supernatant 20% of polyethylene glycol were achieved. By our study we could obtain four different fractions as intermediate sources for protein purification which cannot be easily obtained from plasma fractionation by cold ethanol fractionation.

To determine seed storage protein banding patterns in some bread wheat cultivars and the similarity of banding patterns among different cultivars, an experiment based on seed storage protein electrophoresis (albumin and globulin) was performed. Water and salt soluble proteins were extracted in sixteen wheat cultivars using polyacrylamide gel electrophoresis and banding pattern was obtained. Studied cultivars were Karaj 3, Atila 50, N-8019, Khazar 1, Shahriar, Darya, Chenab, Kouhdasht, Augusta, Toos, Cros shahi, C-845512, Saysons, Ghermezak, Sardari and Tajan. Based on dendrogram, sixteen wheat cultivars were placed in four groups. Cultivars that were placed together in a group were more similar than the others considering morphological characteristics and growth habits. Electrophoretic patterns of seed albumin and globulin proteins in sixteen wheat cultivars showed that these sixteen cultivars are different in terms of protein banding patterns. It means that the albumin and globulin can be used in genetic evaluation to evaluate genetic distances and identify the cultivars. It can also be used for genetic evaluation of seed storage proteins, including investigation of genetic distances and proximity between species and cultivars.

Genus Rattus from the subfamily Murinae has a wide geographical distribution throughout the world. Three species of this genus have been reported in Iran so far. They are Norway or brown rat (Rattus norvegicus), black rat (Rattus rattus), and Himalayan rat (Rattus pyctoris). In order to verify the possibility of using electrophoretic patterns of special proteins, in biosystematic analysis of these species, total proteins of plasma (globulins and albumin) and esterse-1 of nine specimens belonging to three species of Rattus, from five populations were analyzed by native-PAGE (native polyacrylamide gel electrophoresis). Electrophoretic patterns of globulins, albumin, and esterase-1 alleles were different in these species and an unknown species was distinguished by this technique. Also, brown rats from Tehran and Mashhad were discriminated by these methods.