جستجوی مقالات مرتبط با کلیدواژه « Rutilus rutilus » در نشریات گروه « زیست شناسی »

Lead (Pb) is one of the destructive and harmful pollutants and it could have undesirable impact on tissues of organisms. In this study، the effect of various concentrations of Lead on liver and gill tissues were examined. The fingerling Rutilus rutilus were prepared and transferred to laboratory. After keeping the samples in aquarium، they were exposed to concentrations of 0. 1، 0. 2 and 0. 4 mg/l Lead for 48، 96، 144 and 168 hours. At mentioned times، samples were removed from control and treatment aquariums then liver and gill tissues were extracted for damage determination. After staining، prepared slides were studied with magnification of 10 x and 40 x by optical microscope. In damaged tissues، Sinusoidal dilation، Vacuolization، Congestion، Hemorrhage، Nuclear piknosis، Hepatocytes necrosis، Hemosiderin accumulation in melanomacrophage cells and cellular acidophilic changes، Lymphocytic infiltration and focal necrosis of liver and gill، Mucosal hyperplasia، Secondary lamella and filaments edema، Pillar cells necrosis، Chloride cells necrosis، Congestion، Hemorrhage، Inflammatory cells infiltration، Hyperplasia، Hypertrophy، Secondary lamella adhesion، Mucosa accumulation، telangiectasia، Epithelial cells dysplasia and metaplasia to mucosal cells and Distal clubbing in secondary lamella were demonstrated (were observed). The results showed that، when the concentration of Lead Nitrate was increased more severe damages occurred. At 0. 1 mg/l Lead، minimum tissue damages were observed، but with increasing time in 0. 2 and 0. 4 mg/l Lead maximum tissue injuries were found.

This study investigates the effects of replacement of fish oil by two vegetable oils (Sunflower oil and Soybean oil) on growth performance، salinity tolerance، hematological and serum biochemical profile of Rutilus rutilus caspicus over 56 days. Five diets were supplemented with 8% of either fish oil (FO) (control)، sunflower oil (SFO)، and soybean oil (SO) or a combination of 4% FO with 4% SFO and 4% FO with 4% SO، respectively. Triplicate groups of fish (24 fish per tank with an average body weight، of 5. 52 g) were assigned to each diet. Blood samples were taken from the caudal vein of 75 apparently healthy fish at the end of trial. Hematological values of the blood samples were determined using standard techniques. At the end of experiment fish were exposed to salinity stress. The results showed that the best growth performance was in fish fed control diet، but this results were similar to B، C and D groups (P>0. 05). Serum cholesterol and glucose concentrations were significantly higher in fish fed diet containing vegetable oils (P<0. 05). Also the count of red blood cells (RBC)، hematocrit (Ht) and hemoglobin (Hb) in groups fed diet containing SFO and SO were higher than other groups (P<0. 05). There was no significant difference in survival index after 48 hours salinity stress (P>0. 05). These results indicate that fish oil can be replaced completely with alternative vegetable lipids without any serious negative growth performance and health impacts.

A series of specific toxicological effects including bioaccumulation of the pollutant, histological changes and influences on ovary tissue were examined in the Rutilus rutilus after the exposure to graded sublethal concentrations (10, 30, 50 μg Hg/L) of mercury chloride (MC). Histopathological changes, such as telangiectasis, and degenerative, can clearly be observed in the slices from the ovary exposed Roach. A batch of Roach with the mean values of 5 cm in length and 5.5 g in mass were pre-raised in a static system, which was supplied with dechlorinated tap water and constantly aerated. The water qualities were controlled as follows: pH, 7.45; oxygen concentration, 7 mg/L; the water temperature, 21–22 ◦C. All fish were fed twice a day with a commercial ration of biomar. Reagents Appropriate amount of mercury chloride (MC, AR 98%, Merck-Schuchardt) was dissolved in a stock solution of 0.676g HgCl2/L. Based on the 24 h LC50 value (70 ppb) obtained from the acute exposure test, a graded series (10, 30, 50 μg Hg/L) of mercury chloride (MC) were administered to Roach fish (Rutilus rutilus) with 48 fish per group. The chemical exposure experiments lasted for 48, 96 and 144 hours. These designed exposure levels were sublethal, and could induce obvious alteration of tissues abnormalities and disease at the same time.

A series of specific toxicological effects including bioaccumulation of the pollutant, histological changes and influences on muscle tissue were examined in the Roach fish (Rutilus rutilus) after the exposure to graded sub lethal concentrations (10, 30, 50 μg Hg/L) of mercury chloride (MC). Histopathological changes, such as telangiectasis, and degenerative, can clearly be observed in the slices from the muscle exposed Roach. A batch of Roach with the mean values of 5 cm in length and 5.5 g in mass were pre-raised in a static system, which was supplied with dechlorinated tap water and constantly aerated. The water qualities were controlled as follows: pH, 7.45; oxygen concentration, 7 mg/L; the water temperature, 21–22 ◦C. All fish were fed twice a day with a commercial ratio of biomar. Reagents appropriate amount of mercury chloride (MC, AR 98%, Merck-Schuchardt) was dissolved in a stock solution of 0.676g HgCl2/L. Based on the 24 h LC50 value (70 ppb) obtained from the acute exposure test, a graded series (10, 30, 50 μg Hg/L) of mercury chloride (MC) were administered to Roach fish (Rutilus rutilus) with 48 fish per group. The chemical exposure experiments lasted for 48, 96 and 144 hours. These designed exposure levels were sublethal, and could induce obvious alteration of tissues abnormalities and disease at the same time.