جستجوی مقالات مرتبط با کلیدواژه "in vivo" در نشریات گروه "شیمی"

Endometriosis is a gynecological disease in the form of chronic inflammation characterized by the presence of tissue outside the uterine cavity that affects around 176 million women around the world. The pathogenesis of this disease is still unknown, resulting in a lack of standard treatment. People in East Nusa Tenggara, Indonesia, use boiled water from the cortex of Sterculia quadrifida R.Br. as a menstrual laxative and to treat menstrual pain, believed to be associated with endometriosis. The aim of this study was to investigate how a water extract of the S. quadrifida cortex prevents endometriosis lesions from growing in mice (Mus musculus) endometriosis models. This study was conducted as a true experimental research, dividing the mice into a control group and three SWE groups at doses of 200, 400, and 800 mg/kgBW. The findings showed that all SWE doses can significantly reduce endometriosis lesions and NF-κB expression compared to the negative control, with the optimal dose being 800 mg/kgBW. This activity was predicted due to its flavonoid compounds. These findings demonstrate the significant potential of S. quadrifida cortex as a developed herbal medicine for endometriosis treatment.

Over the last decade, engineering the polymeric vascular grafts has been extensively studied. Various types of polymers have been used in this field such as synthetic polymers, natural polymers, and polymer blends. Synthetic polymers, such as Polycaprolactone (PCL), have displayed improved mechanical specifications compared to natural polymers. Polycaprolactone is biodegradable polyester that can be blended with another synthetic polymer or a natural polymer to yield even greater enhanced mechanical properties. The mechanical properties of artificial blood vessels play an important role while the vessels are attached to the native vessels in the animal body. Furthermore, the artificial blood vessels must be adequately strong to resist frequent blood circulation and related pressure. The most significant advantage of engineered vascular tissue implants is their ability to grow, remodel, rebuild, and respond to injury. This article serves as a review of the fabrication, specifications, and benefits of various kinds of polycaprolactone grafts. The primary focus is on the in vivo implantation of nanofibrous ones for vascular regeneration in large and small animals. First, the subject of the study was thoroughly investigated, then the search was conducted with a combination of index and text terms. Finally, a number of articles, scientific books, patents, manuals, and university theses were selected and studied, and the obtained data were analyzed, categorized, and edited. PCL polymer has been the most sought-after biodegradable polymer for use as a vascular tissue engineering material.

Bone substitute materials in the current use sustain several drawbacks such as donor site morbidity, induce immunity, availability, and economic production. New synthetic bone substitutes are needed that overcome these drawbacks. This study aimed to produce a porous geopolymer and evaluate its usefulness as a bone substitute. Metakaolin-based geopolymer with different porosity percentages and sizes was produced by the addition of hydrogen peroxide and olive oil in different ratios to the geopolymer paste. Image J analysis had used to calculate the porosity percentage and size. Geopolymers with the highest porosity percentage and size range were selected for in vivo testing on animals. In the in vivo study, porous geopolymer was implanted in femur bones of 12 rabbits. The right femur of each rabbit served as the positive control (using the commercial bone replacement TEEBON®), and the negative control group received no treatment. The left femur was used to implant the geopolymer. Two and four weeks after implantation, biopsies were performed for histological analysis. After two and four weeks, a histological examination of the implanted material revealed the development of bone trabeculae with minimal inflammation. As compared with commercial bone substitutes, geopolymers improved bone formation. Owing to the results, porous geopolymers could be the promising materials for bone substitutes, as they are available and cost effective.

Generally, traditional bioanalytical methods including in vitro or ex vivo are associated with the limitations and drawbacks in the living systems analysis. However, the in vivo sampling technique is an excellent procedure to improve accuracy and performing the on–line and in–situ biological analyses. In this regard, solid–phase microextraction (SPME) as a simple, sensitive, solventless and noninvasive sample preparation technique has been considered by researchers in in vivo sampling, in recent years. This review briefly describes the use of in vivo SPME as a sample preparation method to study the living systems involving plants and animals (especially metabolomics and clinical researches). Also, biocompatible coatings and design innovations that use to enhance the sensitivity and functioning of the method have been investigated. Finally, the challenges facing the development in vivo SPME method are investigated and forthcoming trends for the better performance of bioanalytical method are offered.