جستجوی مقالات مرتبط با کلیدواژه "pseudomonas aeruginosa" در نشریات گروه "شیمی"

In this work, Pseudomonas aeruginosa was used to produce pyocholin as siderphores compound. This biosynthesis to get this type of siderphorses were performed by force these organisms by some metal ions such as Fe3+, Ni2+, Cd2+, and La3+. The produced siderphoreses were extracted and characterized by FTIR and 1H-NMR. Beside this biosynthesis, the analog siderphorses compound (ethyl (Z)-2-(2-(4-bromobenzylidene) hydrazinyl)-4-methylthiazole-5-carboxylate) was synthesized to study its coordination chemistry with Fe3+, Cd2+, and La2+ by complexation processes. This ligand was synthesized and characterized by FTIR spectroscopy, 1H-NMR spectroscopy, and UV-Vis spectrometry. It was found that the synthesized ligand was a bidentate ligand that was expected geometry as octahedral for the Fe (III) and La (III) complexes while tetrahedral geometry for the Ni (II) and Cd (II) complexes. The importance of the study lies in the creation of compounds capable of mimicking the action of bacterial extracts, which can be applied in several fields including toxic, and heavy metal remediation, iron overload diseases, ẞ thalassemia, iron chelators, anti-malarial, biocontrol agent, and cancer. The synthetic compounds were identified also using IR spectroscopy, 1H-NMR, and UV-Vis spectrometry. This work may contribute to open the door to show the probability using siderphores or their analogues to reduce toxic metals levels, pollutants, and waste of crude oil such as cadmium and mercury in Iraq by microbial organisms.

Nanotechnology-based antibiotic synthesis is one of the most crucial contemporary strategies for preventing antibiotic resistance. Synthesis of nano sulfadiazine antibiotic was nanoscale made by using standard sulfadiazine in this study, Physically, without using any chemicals. The resulting nanocomposite was examined using XRD, EDX, and SEM methods, and their characteristics were contrasted with those of nano sulfadiazine, whose average crystal size was 48.32 nm. The ability of nano sulfadiazine to prevent bacteria growth was examined by measuring the minimum inhibitory concentration of two species of bacteria using an ELISA technique; it was compared to regular sulfadiazine particles. The results of the broth microdilution method with standard sulfadiazine gradient (concentration) ranges of 8-1024μg/ml show the MIC ranging 64-128μg/ml among five MDR P. aeruginosa isolates and five MDR S. aureus isolates. While the results of Nano-sulfadiazine MIC ranged from 16-32μg/ml for P. aeruginosa, isolates and 32μg/ml for S. aureus isolates.

The present study was designed to explore the effect of gold nanoparticles on the hmgA gene expression and pyomelanin pigment production from local Pseudomonas aeruginosa isolates. Out of 162 patients suffering from ear infections, urinary tract infections, burns, wounds, cerebrospinal fluid (CSF), respiratory tract infections (RTI), and blood infection (sepsis), eight isolates identified to produce pyomelanin pigment (8.42%). All isolates were characterized using microscopical, morphological, and biochemical methods, VITEK-2 compact systems, and 16SrRNA gene, which showed that all these isolates belong to P. aeruginosa. Screening producing pyomelanin pigment was carried out by using a specific media to promote the production of pyomelanin pigment. The extracted pyomelanin pigment was purified using simple acid sedimentation followed by centrifugation to extract the crude product and purify it with HPLC. The purified pigment was positive for all major physical and chemical tests that characterize pyomelanin pigment, including UV-visible spectroscopy and Fourier transform infrared spectroscopy (FT-IR). The study also covered the preparation of gold nanoparticles using the green chemistry method, which used black tea-leaf extract. The resulting nanoparticles were positive for all significant qualitative tests used to characterize them, including UV-visible spectroscopy (FT-IR), X-ray diffraction (XRD), and SEM. The results of the SEM image showed spherical particles with a size of approximately 19nm nanoparticles. In conclusion, the effect of the prepared gold nanoparticles on the gene expression of the hmgA gene was studied at different concentrations compared to the control sample using a real-time one-step polymerization reaction, also the gene expression results showed that the gold nanoparticles significantly increased the gene expression of the hmgA gene.

Recently, microbial surface-active molecules called biosurfactants, have gained significant attention due to their structural diversity, biodegradability, low toxicity, and several environmental and industrial applications. However, despite their advantages, they are not widely used because of high production costs, which can be overcome by bioconversion of agro-industrial wastes as low-cost substrates. The current study aimed to overcome the challenges of biosurfactant production by bioconversion of soybean meal, as a low-cost renewable substrate, and to optimize the significant parameters. Rhamnolipid biosurfactant was produced by Pseudomonas aeruginosa (PTCC 1074) using soybean meal under solid-state fermentation and Response Surface Methodology (RSM) by Central Composite Design (CCD) was employed to optimize the significant parameters. The experimental value of biosurfactant production and Emulsification Index were 17.05 (g/kg dry substrate) and 54 % respectively under the optimal conditions (temperature 33 ºC, Initial substrate moisture 80%, and carbon-to-nitrogen ratio (C/N ratio) 54). Regression analysis with RSM resulted in quadratic models and the coefficient of determination (R2), adjusted R2, and predicted R2 were respectively calculated as 0.9767, 0.9557, and 0.9088, indicating that the model fitted the experimental data well.  An increase in temperature from 25 to 34°C led to a rise in rhamnolipid production, which implies the significant influence of temperature. The results demonstrated that  the production of biosurfactants increased with increasing the initial moisture content at high temperatures and also at low C/N ratios. The current study confirmed the considerable potential of soybean meal for biosurfactant production and also enhanced the production yield by optimizing the significant process parameters.

This study aimed to demonstrate the biosynthetic process of iron oxide nanoparticles (Fe2O3-NPs) by using extracellular components produced by environmental isolates of Lactobacillus plantarum as reducing and stabilizing agents studied in the laboratory of the university of Baghdad. Add 1 g of iron sulfate to 10 mL of extracellular nanoparticles for synthesis. Biosynthetic Fe2O3 nanoparticles have broad application prospects in catalysis, biosensing, anticancer, and biomedicine. Optimal conditions for synthesizing Fe2O3 were investigated using UV-VIS, AFM, XRD, FT-IR, and FE-SEM techniques. The UV-VIS wavelength of NPs within the nanoclusters. Susceptibility testing of P. aeruginosa showed resistance to tetracycline, trimethoprim-sulfamethoxazole, ceftazidime, and chloramphenicol, whereas sensitive to Amikacin, Norfloxacin, Meropenem, and Ciprofloxacin and the effect of Fe2O3 NPs from extracellular component on bacteria Pseudomonas aeruginosa on an inhibition zone 18 mm.

Groundnut shells (GSs) are abundant renewable by-products which have been underexploited for potential applications. Therefore, this paper reports the bioactive potential of groundnut shell extracts (GSEs) against Staphylococcus aureus and Pseudomonas aeruginosa. The GSs were ground into powder form and subjected to extraction using ethanol, ethyl acetate, and a mixture of ethanol and ethyl acetate using an electrical shaker for 6 h and 12 h; and subsequently centrifuged at 2000 rpm for 20 min. The GSEs were then qualitatively screened for phenol, quinone, saponin tannins, and flavonoids using the standard procedures. More so, antibacterial activities of these GSEs against P. aeruginosa (ATCC 29953) and S. aureus (ATCC 25923) were tested using Agar well diffusion method on Mueller-Hinton agar (MHA). Therefore, the preliminary phytochemical screening reviewed the presence of saponin, tannin, flavonoid, quinone, and phenol. And the investigation of the antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa demonstrated that S. aeureus was more sensitive to attack by the EtOH derived GSEs; whereas, P. aeruginosa was readily affected by the EtOAc GSEs. Generally, P. aeruginosa was more inhibited by these GSEs even at the lower concentrations of 25 and 12.5 mg/ mL; especially with the EtOH + EtOAc and EtOAc derived GSEs. EtOH + EtOAc GSE has potential of enhancing these bacterial inhibitions

Abuse of antibiotics in therapy has led to development of resistance in the target organisms. Failure of the current antibiotics to control infections makes it essential to discover alternative drugs. The pathogenicity in numerous bacteria is regulated by Quorum sensing (QS) signaling systems. The QS inhibition system may cause the reduction of virulence and defense against the bacterial infections. The QS is the main regulator of virulence and biofilm formation in Pseudomonas aeruginosa. A variety of plants showed their effects on P. aeruginosa virulence. Extract of various plants control the regulatory QS genes and factors with marginal effects on bacterial growth. The quorum-quenching (QQ) mechanisms are unrelated to static or cidal effects. In fact, anti-QS have already shown promise in the battle against P. aeruginosa infections.