جستجوی مقالات مرتبط با کلیدواژه « Genotoxicity » در نشریات گروه « شیمی »

A chemotherapeutic agent known as the etoposide is used to treat acute lymphoblastic leukaemia (ALL). Over the years, the etoposide usage in treating ALL has yielded positive outcomes. Nevertheless, it has been discovered that a number of ALL patients experience side-effects and are susceptible to cancer cells. Accordingly, drug research for improved chemotherapy becomes necessary. The results of numerous investigations using compounds containing organotin (IV) dithiocarbamate were encouraging. The objective of this study is to examine the genotoxicity effects on Jurkat E6.1 cells, derivatives of the aforementioned substances were chosen for this investigation. The assessment of cell cycle arrest and genotoxic effects on Jurkat E6.1 cell lines was accomplished using the substances triphenyltin (IV) diisopropyldithiocarbamate (C1) and triphenyltin (IV) dialkyldithiocarbamate (C2). The cell cycle arrest was established using cell cycle analysis. The average DNA tail moment score was used to calculate the genotoxic effects of DNA damage. Inhibitory concentration (IC50) was used to conduct both analyses. C1 has an IC50 of 0.1 M, while C2 has an IC50 of 0.2 M. On the other hand, the etoposide, which served as a positive control, has an IC50 of 0.87 M. The research findings demonstrated that after four hours of treatment, both triphenyltin (IV) dithiocarbamate and etoposide compounds prompted cell cycle arrest, with a significant difference (p < 0.05) in the S phase. Following a four-hour etoposide treatment, the findings of genotoxicity assessment revealed substantial DNA damage (p < 0.05). Furthermore, the C1 and C2 treatments revealed a similar level of DNA damage. However, when a statistical analysis of the comet assay results was accomplished, no discernible change (p > 0.05) was observed. In summary, DNA damage and cell cycle trapping were caused by etoposide, C1, and C2 in Jurkat E6.1 cells.

Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants in the Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential.  The present research aimed to investigate the induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspension of noscapine. Culture of HepG2 cells as spheroids was treated with different concentrations of noscapine for 24 h on Day 11. Afterward, viability assay and alkaline comet assay methods were applied to examine the viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween 40 on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline.

Zinc oxide (ZnO) nanoparticles have received growing attention for several biomedical applications. Nanoparticles proposed for these applications possess the potential to interact with biological components such as the blood, cells/ tissues following their administration into the body. Hence we carried out in vivo investigations in Swiss Albino Mice to understand the interaction of ZnO nanorods with the biological components following intravenous and oral routes of administration to assess nanoparticles safety. Intravenously injected ZnO nanorods were found to induce the significant reduction in the red blood cells and platelet counts. Elevated levels of serum enzymes such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase were observed following intravenous and oral administration. Also, increased levels (p