جستجوی مقالات مرتبط با کلیدواژه « جنبایی » در نشریات گروه « محیط زیست »

The most important factors involved in sperm fertility are motility, morphology and sperm membrane integrity. The studies show that, the addition of Pentoxifylline(PTX) can increase sperm motility by increasing energy production. The aim of the present study was to investigate the effect of adding Pentoxifylline to rooster semen on qualitative parameters of rooster sperm after 6 hours of storage at 4°C. Semen samples were collected from 15 Ross 308 roosters at 28 weeks of age. After initial evaluations, semen samples were mixed together to eliminate individual differences. After diluting the semen and adding different levels of Pentoxifylline (0, 0.25, 0.5, 0.75 and 1 μM), the samples were kept at 4°C for 6 hours. Then, sperm kinetic parameters were evaluated. The results showed that the addition of 0.75 μM PTX significantly improved progressive mobility, curvilinear velocity, viability and plasma membrane integrity of spermatozoa compared to the control group (P<0.05).  In overall, it can be concluded that methylxanthine-derived drugs such as Pentoxifylline can have a significant effect on the motility, viability and plasma membrane integrity of rooster sperm in the short period of time.

The aim of the present study was to investigate the effect of ethanol on bull sperm quality after freeze thawing process. Treatments used in this study included: 1) semen extender without ethanol as control group (C) and 2) semen extender containing 0.25% ethanol (E). A total of 24 ejaculations were collected from four healthy mature Holstein bulls in 3 consecutive weeks and twice a week. In each replicate, semen samples were mixed to eliminate individual differences and then divided into two equal parts and each of them were extended with one of the aforementioned extenders. Afterward the treatments were frozen using a standard protocol. After thawing, sperm motion characteristics, plasma membrane integrity and functionality, apoptosis status and mitochondrial activity were assessed. The result of this study was showed that spermatozoa in control group had higher total motility (68.5±1.96 vs 76.17±2.49) and lower apoptosis (10.65±0.36 vs 13.13±0.33) in comparison with group treated with ethanol (p≤0.05). Also, no differences were observed in plasma membrane integrity and functionality and mitochondrial activity between two treatments (p≥0.05). In conclusion, the results showed that ethanol could negatively affect post thaw characteristics of bull spermatozoa.