جستجوی مقالات مرتبط با کلیدواژه « cytochrome oxidase i » در نشریات گروه « محیط زیست »

The molecular phylogeny of Vetigastropoda species have been studied based on mitochondrial genes, COI and 16S rRNA in the present work for the first time in the northern rocky coastal zones of Persian Gulf during years 2013 and 2014. After morphological identifications of species, DNA extraction, amplifying partial of cytochrome oxidase COI and 16S rRNA and sequencing procedure were done in the laboratory. In this study 9 COI and 5 16S rRNA sequencing belonging to 5 species were measured. Also, phylogeny analyses with drawing phylogeny trees of Maximum Likelihood, Maximum Parsimony and Bayesian were done using MEGA6, PAUP and BEAST softwares. The result showed that clade Vetigastropoda, include Trochidae, Chilodontidae, Turbinidae and Fissurellidae families, is not monophyletic, which is not as classic identification. So Trochidae and Chilodontidae are in seprated clade from Turbinidae and Fissurellidae clade᾽s. This showed not monophyletic relation between this two group which represented difference between classic and molecular classification.

The present study investigated the biodiversity of Nayband beach by using molecular and morphological studies. For this purpose, samples were taken from dominant bivalvia in sandy shores of Bushehr, Nayband in April 2016. To record morphological features such as shell shape, decorating on the shells, umbo shape, position,numbers and shape of valve teeth was photographed. For molecular studies, DNA was extracted by using CTAB method and mitochondrial Cytochrome oxidase subunit gene segment (COI) was PCR and sequenced. results of morphological identification showed that all the samples are most likely belong to Callista genus, C.umbonella Species and from the Veneridae families. But sample NI9, the blast of sequences from sample present study showed the highest percentage of similarity with Turtonia minuta (DQ184850) from Veneridae family. however, the sample NI9 is most closely resembles with Costacallista impar species (DQ184808) species showed that this species is also belongs to Veneridae family. phylogenetic trees and alignment of these sequences in the gene bank was only able to prove their belonging to the veneridae family. results evolutionary tree topology for all three analyzes (Maximum parsimony, Maximum likelihood,) per 17 sequences showed that the nucleotide sequence of the COI gene, in all samples that presented as one species, are not the same. although, there were very similar morphology in the samples, the genetic differences among trees drawn between these samples revealed that it should be considered the possibility of creating new species and further studies and more detailed studies of morphology and molecular markers presented more decisive idea.

Neogastropoda is one of Caenogastropoda’s orders belongs to Gastropoda class. There are some families of this clade in the Persian Gulf. The phelogeny and divergence time of them have been studied for the first in the northern rocky coastal zones of Persian Gulf during years 2013 and 2014. After morphological identifications of species, DNA extraction, amplifying partial of cytochrome oxidase COI and 16S rRNA and sequencing procedure were done in the laboratory. In this study 7 COI and 3 16S sequencing belonging to 4 species were measured. Also, phylogeny analyses with drawing phylogeny trees of Maximum Likelihood, Maximum Parsimonyand Bayesian were done using MEGA6, PAUPand BEAST softwares. Results showed that the members of Neogastropods in the Persian Gulf have been divergent in middle Eocene; So that their common ancestor astimatedin about 47 million years ago. In addition Neogastropoda is a monophyletic clade.