جستجوی مقالات مرتبط با کلیدواژه « Paracetamol » در نشریات گروه « میان رشته ای »

Pharmaceutical formulations for cold symptoms’ relief usually contain high concentration of acetaminophen and small concentration of phenylephrine hydrochloride and chlorpheniramine maleate. The combination makes a problem in simultaneous quantification of the chemicals. Combination of paracetamol, phenylephrine hydrochloride, chlorpheniramine maleate, and caffeine is widely used for analgesic, antipyretic, antihistamine, and antitussive activity. At this study, a simple reversed phase high-performance liquid chromatography was developed and validated for determination of chlorpheniramine maleate, phenylephrine hydrochloride, and paracetamol in pharmaceutical formulations at the same time.

One ml of stock solutions were diluted with mobile phase to prepare final concentrations of the all three chemicals (20 mg l-1 of chlorpheniramine maleate, 325 mg l-1 of phenylephrine hydrochloride, and 50 mg l-1 of paracetamol). The formulations were prepared by addition of the chemicals to a volumetric flask. Then, they were made up to 100 ml with mobile phase of 0.05 M phosphate buffer:acetonitrile (95:5). pH of the mobile phase was adjusted to 2.5 with 50% orthophosphoric acid. Analysis was done in 250 mm ´ 4.6 mm ´ 5 µm C18 column. 

Results and conclusion:

Based on the results, the flow rate was 1.5 ml min-1 during isocratic elution of the samples. The analytes were detected at 210 nm by UV detector. Retention time of the last eluted analyte was 8 min. The method was validated according to ICH guidelines. The results revealed that the proposed method is reliable and accurate. Therefore, it can be applied for routine analysis of tablets in order to control their quality and stability.