جستجوی مقالات مرتبط با کلیدواژه « Suspension culture » در نشریات گروه « علوم پایه »

In this research, the effect of type and concentration of BAP and 2,4-D plant growth regulators on callogenesis of hypocotyl and leaf explants of Portulaca oleracea in MS medium containing 0.5, 1 and 2 mg/L of 2,4-D and BAP and optimization of cell suspension culture with different plant growth regulators in MS medium containing 0.5, 1 and 2 mg/L 2,4-D, 0.5 and 1 mg/L BAP, 0.2 and 0.5 mg/L Kin each one containing 20 and 30 g/l sucrose concentration, were investigated. Based on the results, both of the samples had 100 percentage callus induction, but the function of hypocotyl callus was significantly higher than the leaf callus. MS culture media containing 2 mg/l BAP and 0.5 mg/l 2,4-D, 2 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 0.5 mg/l 2,4-D, 0.5 mg/l BAP And 0.5 mg/l 2,4-D and 1 mg/l BAP and 2 mg/l 2,4-D, all containing 150 mg/l ascorbic acid in both explants have the highest yield (wet weight of callus). In the cell suspension culture, there was a significant difference between treatments with the plant growth regulators in terms of number of cells in per ml, SCV, PCV and treatment duration.

The red-fleshed apples are from varieties of apples confer the competence and high ability in vitro to produce anthocyanins in their organs. Several factors affect the in vitro production of anthocyanins in plant tissue culture and to produce high, the optimizing of these factors are very necessary. Light is one of the most important factors that many studies have been done in this area but the laser irradiation effects has not been investigated. We have studied the effect of combine lasers treatment of He-Ne with diode on in vitro production anthocyanin and cellular growth of cell suspension of red-fleshed apple. This experiment with 18 combinations treatments (blue laser at the intensities of 67.09, 32.74, 30.4 and 13.73 mW/cm2 and a red laser at the intensities of 6.46, 4.82, 1.54 and 0.666 mW/cm2 and two controls including darkness and fluorescent light) as a factorial design based on CRD with three replications for each treatment were applied.With increasing of combination lasers radiation intensity, production rates monomeric total anthocyanins (TMA) and total anthocyanins (TA) increased but lead to was reduced the cell growth. Results of this work showed that probably high intensity of combination lasers radiation treatments as a stress factor made decreased cell growth and induced high concentration of anthocyanins in the cell exposed to stress and good for decreasing injures proceed from stress and also lead to persevered cell of red-fleshed apple through high concentration accumulation of anthocyanin in vacuole.

Tobacco (Nicotianatabacum L.) is known as a model plant in tissue culture and genetic engineering from the past decades. In this study the effect of ultrasonic waves on viability and disruption of tobacco cells were investigated. For callus induction, tobacco leaf explants cultured on MS medium supplemented with 2,4-D 1.5 mg/l and 1 mg/l Kinetin. The cell suspension culture was initiated by transferring of calli into liquid medium. Then the cell suspensions exposed to ultrasonic wave with different levels of temperature and duration. Cell viability was determined by Trypan blue staining, and structural and ultra-structural disruptions in cell wall were evaluated using optical and scanning electron microscopy (SEM). The results showed that the viability of cells significantly influenced by temperature and duration of ultrasonic wave treatments. The highest cell viability (%76.702) was obtained with 2 min sonication at 25 or 40 °C, while, the highest cell wall disruption were observed at 50° C with 14 min sonication (% 18.733) and at 25° C with 20 min of ultrasonic treatment (% 17.48). Scanning electron microscopy (SEM) was confirmed the formation of different sized pores on cell wall under ultrasound treatment.

Neospora caninum is one of main etiologic factors of abortion in  in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost effective for mass production of biological products, this study aimed to evaluate the suspension cell culturing for production of this protozoan.

This study was experimentally performed bases on growth of N. caninum tachyzoites on Ka6 cell line (a cell line obtained from cattle infected with Theileria lestoquardi, Razi Institute, Shiraz, Iran. Next, based on MTT assay, ability of this cell culture for production of  N. caninum was compared with  Vero cell as the best current cell line for this purpose.

The results showed that N. caninum tachyzoites could enter into K6a cell lines and after replication released from the cells successfully. Replication of the tachyzoites was significant in both Vero and K6a cell lines in compare to the control.

To our knowledge, this is the first and successful report of suspension culture of N. caninum tachyzoites. Although, the rate of N. caninum proliferation on Ka6 cell line did not show any significant difference in compare to Vero cell line, since Ka6 cell line is a transformed lymphocytic cell and it is possible to grow massively this cell line as suspension bioreactors, it is preferred to Vero cell line.