جستجوی مقالات مرتبط با کلیدواژه "بنزیل ایزوکوئینولین ها" در نشریات گروه "کشاورزی"

The greater celandine (Chelidonium majus L.) contains important alkaloids such as Sanguinarine. Sanguinarine is an active alkaloid with potentially antimicrobial, anti-inflammatory and anti-tumor properties that is widely found in plants of Papaveraceae family. In this research, the isolation and sequencing of (s)-cis-N-methyl stylopine 14-hydroxylas (MSH) coding gene was performed as one of the key enzymes in Sanguinarine pathway from celandine. Then, the changes in cmMSH gene expression were investigated in roots, leave and stems at four salinity levels (0, 25, 50, and 100 mM) in a factorial experiment based on completely randomized design. In the present study, the cDNA encoding MSH enzyme was isolated from root and successfully integrated into PTG19-T plasmid and cloned at E. coli. After confirmation of recombinant clones by PCR, plasmids of recombinant bacteria were extracted and the gene segment sequenced. The sequenced segment had 784 nucleotides with an open reading frame of 261 amino acids which the derived protein with functional domains including helix K region, heme-binding region and aromatic region belonged to the Cytochrome-P450 protein family. In the phylogeny tree, the protein sequence of isolated cmMSH gene had the most similarity with methylstylopine 14-hydroxylase enzyme of poppy species. Mean comparison of gene relative expression showed that 50 mM salinity had maximal effect on increasing of cmMSH gene expression in root tissue. Also, the expression pattern showed that with increment the salinity up to 50 mM, the expression of cmMSH gene increased in leaves and roots, but with increment the salinity to 100 mM the gene expression decreased 35% in both tissues.

Phytohormones and PGRs are influencing factors in the production of secondary metabolites. Papaverace family includes valuable alkaloids with commercial importance and Papaver bracteatum belongs to this family. Benzylisoquinoline alkaloids are a diverse group of more than 2500 specified natural compounds with medicinal effect which are found in 5 plant families including Papaveraceae. In vitro plant cell culture is a promising source for high rate secondary metabolites production. In this study, the effect of methyl jasmonate (MJ) and phloroglucinol (PG) in three different concentration, 0, 100 and 200 uM and mg/L, individually and in combination together, on the stimulation of thebaine and sanguinarine production in Papaver bracteatum cell suspension cultures was studied. Both elicitors significantly increased yields of thebaine and sanguinarine production compared to the control. Thebaine production 48 hours after elicitation with concentration of 200uM MJ and 100mg/L PG was about 68.63-fold and sanguinarine production at the same period with concentration of 200uM MJ and 200mg/L PG increased about 107.71-fold more than control. It seem that increases can due to synergic effects of used elicitors in this suspension cell culture. Adding MJ and PG, each one lonely, also increased yields of thebaine and sanguinarine. The results of this study showed that the use of PGRs can motivate biosynthetic cycle of the production and accumulation of morphinan alkaloids in plants and levels of this alkaloids can be eleveted comparing to control plants.