جستجوی مقالات مرتبط با کلیدواژه « شنبلیله » در نشریات گروه « کشاورزی »

Various species of genus Trigonella are important from medical and food aspects.Assessment of genetic diversity is an important principal for plant breeding, providing an opportunity to identify the novel genetic characters and new genes for breeders. In the current work, 90 fenugreek ecotypes were evaluated for genetic diversity using several start codon targeted polymorphism (SCoT) and universal rice primer (URP) markers. Ten SCoT and 10 URP primers amplified a total of 100 and 109 fragments, respectively, which out all fragments were polymorphic. The mean values of polymorphic information content (PIC) for both marker systems was 0.34. However, the highest means values for MI and Rp parameters were estimated for URP compared with SCoT markers. The results of the molecular analysis of variance (AMOVA) based on SCoT, URP, and SCoT+URP data showed that the genetic variation within populations is more than between them. Based on genetic variation parameters, the highest number of observed alleles (Na), Shannon’s information index (I), Nei’s genetic diversity (H) and the percentage of polymorphic loci (PPL) were found in T. foenum-graecum and T. monantha species. Cluster analysis based on each marker and pooled data clustered all investigated samples into three main clusters. Furthermore, our results indicated that the grouping pattern obtained by pooled data is in accordance to taxonomic structure of species. In conclusion, our results revealed the high rate of genetic diversity within fenugreek ecotypes. Hence, this germplasm can be used as a valuable gene source for breeding programs.

To evaluate the effect of living mulches on weed control and yield of Black cumin (Nigella sativa L.)  weeds by living mulch, a factorial split experiment has been conducted in the form of a randomized complete block design with four replications in the research farm of Aburaihan Campus, the University of Tehran in the 2017-2018 crop year. Factors of seedbed planting (stale seedbed and simple), type of living mulch (berseem clover (Trifolium alexandrinum L.), fenugreek (Trigonella foenum-graecum L.), and alfalfa (Medicago sativa L.), as well as planting time of living mulch (two weeks before and at the same time of black seed cultivation). The first factor is in the main plots and the combination of the second and third factors, in the subplots. Results show that fenugreek and alfalfa have been more successful in terms of weed control than clover with a 40% reduction in weed density and biomass, compared to the control without either live mulch or weeding. Also, in order to achieve the highest percentage of black cumin yield traits (83.02, 93.44, and 50.50), the number of capsules per plant, number of seeds per capsule, and 1000-seed weight are compared to the control. It is recommended to cultivate fenugreek at the same time as black cumin. In case of alfalfa, pre-sowing is also recommended. Stale seedbed planting system does not play a positive and significant role in weed control and increase in black seed yield traits. It is also better to grow fenugreek at the same time as black seed to get more seed yield in black cumin, but in case of alfalfa, it is better to plant earlier than black seed in the absence of any significant difference among planting times. It seems that alfalfa and fenugreek control weeds by accelerating canopy closure, increasing weed competition with black seed, not interfering negatively with black seed growth, and providing nitrogen to black seed have increased its yield.

The aim of this study was to investigate the effect of 24-epi-brassinosteroid on some of the physiological properties of fenugreek under high temperature stress. The treatments consisted of 24-epi-brassinosteroid (2, 5 and 10 ppm), different harvest times (6 and 24 h) and high temperature stress (42° C). Results showed that high temperature stress reduced chlorophyll a, b and total chlorophyll and carotenoid contents and increased malondialdehyde content and electrolyte leakage.  24-epi-brassinosteroid treatment during stress significantly prevented carotenoid, chlorophyll a,  b and total chlorophyll contents to be deceased.  Applying different levels of 24-epi-brassinosteroid (2 and 5 ppm) at high temperatures decreased malondialdehyde and increased fenugreek tolerance, due to the reduced non-fatty acid peroxidation. The activity of catalase, guaiacol peroxidase and ascorbate peroxidase enzymes enhanced in stress condition and application of 2 and 5 ppm 24-epi-brassinosteroid resulted in further increase of these enzymes activity. The application of 24-epi-brassinosteroid resulted in increased defense gene activity and enhanced content of antioxidant enzymes including ascorbate peroxidase, catalase and guaiacol peroxidase by stimulating the plant defense system. Overall, the results of this study showed that 24-epi-brassinosteroid stimulates the activity of genes involved in either photosynthesis or the antioxidant defense system of fenugreek, and fenugreek tolerance to high temperature stress increased, by reducing oxidative stress.

The application of various stimulants, such as abiotic stress, radiation, and growth regulators to increase the quantity of secondary metabolites is one of the interesting research areas. The aim of this study was to investigate the effect of 24-epi-brassinosteroid on the expression of some genes involved in the diosgenin biosynthetic pathway of fenugreek under high temperature stress. The highest expression of cycloartenol synthase (27-fold) was observed at high temperature treatment and 2 ppm of 24-epi-brassinosteroid (at 24 h after heat stress), while the highest expression of SSR gene was 3.5 fold at normal temperature and 5 ppm 24-epi-brassinosteroid. Application of 2 and 5 ppm of 24-epi-brassinosteroid significantly increased squalene epoxidase expression during high temperature stress. Compared to normal condition, squalene epoxidase expression increased 20 and 10 fold at 2 and 5 ppm of 24-epi-brassinosteroid, respectively. Application of 24-epi-brassinosteroid (2 ppm) after 24 hours significantly increased the expression of sterol methyltransferase by 6-fold (Compared to 23°C and non-application of 24-epi-brassinosteroid). Interestingly, compared to normal condition, squalene synthase expression was decreased at high temperature treatment and different levels of 24-epi-brassinosteroid. The results showed that 24-epi-brassinosteroid (2 and 5 ppm) could be used as a suitable stimulus to enhance the expression of some genes involved in the biosynthesis pathway and subsequently to increase the content of the diosgenin.

The purpose of this study was to determine the effect of fenugreek planting as a living mutch on the phenology, grain quality and yield of sunflower cultivars and to determine the most appropriate time for planting of sunflower. 

This experiment was done in 2016-17 growth season at Razi University. The experiment was conducted as split plot based on randomized complete block design. First factor was the sowing fenugreek at four levels (control (without living mulch), 15 days before sowing of sunflower, simultaneous sowing with sunflower, 15 days after sowing of sunflower) and the second factor was three sunflower cultivars (Progress, Farrokh, Lakomka). 

Planting of fenugreek as living mulch pre-sunflower led to a decrease in the quantitative and qualitative yield of sunflower. In 15 days before sunflower condition, grain, oil and protein yields of sunflower,were 38.5%, 37.9% and 43.6% lower than control treatment, respectively. In addition, the phenological stages of sunflower growth was delayed in pre-sunflower sowing conditions. So that the occurrence of staring, flowering, head formation and physiological maturity were 69.8, 21.8, 24.5 and 82.6 days, respectively (4.4, 1.1, 1 and 3 day) more than control conditions. However, planting of living mulch simultaneous and 15 days after sunflower had a positive effect on the quantitative and qualitative yield of sunflower grains. Grain, oil and protein yields of sunflower were 39.4%, 36.4% and 43.3% higher than control, respectively. These values were 25.5%, 26.7% and 23.5% after sunflower planting, respectively. Flowering and head formation stages in simultaneous planting conditions were 20.8 and 47.1 °C (0.7 and 1.4 days, respectively) and in post-sunflower sowing conditions were 42.3 and 122.6, respectively. Day growth rates were lower (1.8 and 4.6 days) than control conditions. Among sunflower cultivars, the occurrence of different phenological stages was higher for Progress cultivar than for Farrokh and Lakomka cultivars. Farrokh had the highest oil content (36%). The highest grain yield (3426 kg.ha-1), oil yield (1203 kg.ha-1) and protein yield (536 kg.ha-1) were allocated to Lacomka cultivar. 

It seems that competition between fenugreek plants and young sunflower plants seems to lead to poor vegetative growth and delay the time of phenological growth stages of sunflower by early sowing of mulch. These results confirm the importance of selecting the appropriate cultivar and minimizing competition by choosing the optimal planting time of living mulch, which gives the pottage-assisted effect a competitive advantage.

This study was done to investigate the response of vegetative traits of sunflower cultivars in the presence of fenugreek as living mulch at Razi University in 2017. The experiment was conducted as split-plot based on randomized complete block. First factor was the sowing fenugreek at four levels (control (without living mulch), 15 days before sowing of sunflower, simultaneous sowing with sunflower, 15 days after sowing of sunflower) and the second factor was three sunflower cultivars (Progress, Farrokh, Lakomka). Results showed that with planting of fenugreek before sunflower, height, stem weight, number of leaves, leaf weight, leaf area index, content of chlorophyll a, b and total of chlorophylls were decreased by 26.4%, 62.1%, 24.8%, 68.5%, 63%, 7.4%, 3.7% and 6.8% compared to control, respectively. The lowest relative water content was also obtained by planting fenugreek before sunflower (65.2% and 52.7% at the 1/3 of primary growth season and flowering stages). The effect of presence mulch under the simultaneous and 15 days after sunflower conditions had no significant effect on growth characteristics of sunflower compared to control condition. Among thecultivars, the highest of grain yield was assigned to Lacomka (3426 kg/ha). However, Progress had the highest height (212 cm), stem weight (523 g/plant), leaf number (25.5 per plant), leaf weight (157.4 g/plant), leaf area index (6.2) and relative water content (77.8% and 70.5% at the 1/3 of of primary growth season and flowering stages). The highest of leaf area ratio (0.013 m2/g) and carotenoid content (2.5 mg) belonged to Farrokh.

Medicinal crops production is well interested in Iran due to market demand in recent years. Silybum marianum L. is an annual or biennial plant, native to the Mediterranean region, which now could be found in other warm and dry regions. Silybium marianum is used traditionally as a hepatoprotective agent and supportive treatment of liver disorders. Its fruits contain flavonolignan compounds, silymarin, which silybin is the main constitute (Abdolahzareh et al., 2012). Management of planting date and integrated nutrition systems in medicinal plants have to be considered. Sowing date affects the crop yields through changing weather parameters in the growth period. In addition to planting date, fertilizers rate and type, has an influence on crop production. Today continuous and excessive application of chemical fertilizers may have negative impacts on soil health and environment causing environmental problems (Mohammadpour Vashvaei et al., 2017). Bio-fertilizers are considered as sustainable alternative sources of nutrients compared to the synthesized fertilizers. The objective of the present study was to examine the effects of sowing date and fertilizing system (chemical, biological and integrated) on the morphological traits and yield of the milk thistle under Birjand, Iran conditions.

This research was carried out at the research farm of Birjand branch, Islamic Azad University, Birjand, Iran, in 2018. The experiment was conducted as a split plot based on a randomized complete blocks design with three replications. Three sowing dates (March 6, April 4 and May 5) and 6 fertilizing systems (non-fertilizer as control, NPK, vermicompost, Akadin, 50% NPK + 50% vermicompost, 50% NPK + 50% Akadin) were as main plots and sub plots respectively. In the study the responses of some morphological traits and yield of Milk Thistle were evaluated. Soil texture in the site of study was loam. Soil pH and EC were 7.4 and 2.3 mmos/cm respectively. Nitrogen, P, and K content of the soil were 3.8, 11.6 and 75.2 mg/kg at the depth of 0-30 cm, respectively. All data were analyzed by SAS statistical software and means were compared by LSD Test at 5% level.

The first planting date (March 6) had the highest average morphological traits (plant height, capitol diameter and number of branches) and seed yield components (capitol number per m2, number of seed per capitol, 1000-seed weight, seed yield). On the other hands the treatments 50% NPK and 50% vermicompost fertilizers had statistical superiority for most of the studied traits. The highest (3327.7 kg/ha) and the lowest (318.4 kg/ha) seed yield were related to the treatments NPK+ vermicompost at the first planting date and treatment of non-fertilizer application at the third planting date, respectively. It seems that seed yield declining for the planting date of May 5, was related to the shortening of growth season and the coincidence of pollination with summer heating. This caused a severe decrease in the number of capitol per m2 and the number of seed per capitol. Normally, using organic fertilizers with chemical fertilizers improves physical aspects of the soil; increases microorganisms activity in the soil, nutrients availability, photosynthesis, yield components and eventually seed yield (Mohammadpour Vashvaei et al., 2017). Similar results also have been reported by Zarghani et al. (2019). Morphological traits quantity positively correlated to yield components and seed yield. Thus the highest value for these traits were observed for the first planting date under using NPK+ vermicompost. As a result, the superiority of seed yield of Silybum marianum in this treatment compared to other treatments, was to be expected. The results of regression analysis showed that traits capitol diameter, number of capitol per m2, number of seed per capitol and 1000-seed weight were entered into the regression model, which explained 84.57% of total seed yield changes.

In general, the planting date of March 6 was superior to the other two planting dates and this planting date could be suggested as a suitable date for the cultivation of Milk Thistle in Birjand region under using 50% NPK+ 50% vermicompost.

Salicylic acid is an important signal molecule modulating plant responses to abiotic (drought) stresses. A pot factorial experiment based on completely randomized design with three replications was carried out to investigate the effect of salicylic acid on yield and essential oil content of fenugreek under water deficit conditions. Factors were two irrigation levels (50 and 100% of field capacity) and four salicylic acid doses (0, 0.5, 1 and 1.5 mM). Results showed that interaction effect of water stress levels and salicylic acid doses was significant (P≤0.01) for number of pods per plant, 1000-seed weight, protein percent, proline and total soluble sugars. Maximum grain yield (119.2 g/m2) was obtained with application of 1.5 mM salicylic acid and water deficit stress showed significant difference with respect to optimum irrigation. Maximum protein percent (13.83%) was obtained under water deficit conditions and without application of salicylic acid. There was no difference in essential oil percent in 1 mM foliar application of salicylic acid between optimum irrigation (1.4%) and water deficit conditions (1.44%). Therefore, with respect to different responses of studied traits to different levels of salicylic acid, it could be concluded that salicylic acid is able to reduce and adjust the negative effects of water deficit stress on fenugreek plant.

The effect of fenugreek as living mulch on Grain yield, yield components and some morphological traits of sunflower, were evaluated by factorial experiment based on randomized complete block design with three replications at the Research Farm of Faculty of Agriculture, Razi University during the 2016-2017 growing season. Treatments were sowing of fenugreek (Trigonella foenum-graceum L.) as living mulch in four levels of control (without living mulch), 15 days before sowing, simultaneous sowing and 15 days after sowing of sunflower and three cultivars of sunflower (Farokh, Progress and Lakomka). The result showed that head diameter, number of head per plant, plant height, grain and biological yields of sunflower varieties were significantly affected by living mulch treatments. The highest head diameter (20.2 cm), number of head per plant (2.04), grain (3834 kg.ha-1) and biological yield (14150 kg.ha-1) were obtained by simultaneous sowing of fenugreek with sunflower. The simultaneous sowing of fenugreek with sunflower increased grain yield by 39% compared with control, while sowing fenugreek 15 days before sowing of sunflower reduced the grain yield by 2% compared to the control. Among sunflower cultivars, the highest grain yield (3426 kg.ha-1) was achieved by Lakomka cultivar. Genarally, the results showed that selection of suitable cultivar and sowing date of living mulch could improve the sunflower yield. Regarding the effect of fenugreek sowing date on sunflower traits and the different responses of cultivars studied, simultaneous sowing of fenugreek with sunflower Lakomka had the highest grain yield in experimental climatic conditions.