جستجوی مقالات مرتبط با کلیدواژه « تجمع نیترات » در نشریات گروه « آب و خاک »

Application of agricultural waste composts, in addition to improving soil fertility, has positive effects on the quality of agricultural products and the environment by reducing the use of chemical fertilizers and recycling agricultural waste. Spinach (Spinacea oleracea L.) is a suitable plant for studying the effects of composts and chemical fertilizers due to some physiological characteristics such as high antioxidant activity and oxalic acid, significant amount of mineral compounds and vitamin C, and nitrate accumulation. Despite relatively extensive studies on the effect of different composts on plants, no study has been conducted so far to investigate the effect of grape pomace (GP) composts on plants in Iran. Therefore, the objectives of the present study were: 1- to investigate the effect of different GP composts on yield, nutrient elements, and some physiological parameters of spinach in comparison with two levels of urea fertilization in a pot experiment in two consecutive growing seasons, and 2- to investigate the relationship between nutrient elements and physiological indicators of spinach based on principal component analysis.

To investigate the effects of GP composts on yield, nutrient elements, and physiological parameters of spinach (Persius hybrid), an outdoor pot experiment was conducted in a randomized complete block design with eight compost treatments, two levels of urea fertilizer (46%), and a control treatment (C0) in three replications and two consecutive growing seasons (spring and fall). Compost treatments included: High grape pomace (HG) (60-63%) with chickpea straw and alfalfa (HG-Ch-A), high GP with chickpea straw and sugar beet pulp (HG-Ch-B), high GP with alfalfa and sugar beet pulp (HG-A-B), high GP combined with chickpea straw, alfalfa, and sugar beet pulp (HG-All); four other compost treatments included low level of grape pomace (LG) (37-42%) combined with other residues/wastes similar to the first four treatments (LG-Ch-A, LG-Ch-B, LG-A-B, and LG-All). Urea fertilizer treatments included: 150 kg per hectare (C150) (two-stage top dressing) and 500 kg per hectare (C500) (three-stage top dressing). Prior to planting, the composts were separately mixed into the soil (sandy loam) at a rate of 2% by weight(. The first crop was grown for 50 days in May 2018 and the second crop was grown for 45 days in September 2018. In both seasons, plant samples were taken in the early morning at the end of the growing season to determine the fresh and oven-dried weight of shoot and root samples, leaf area, nutrient elements, and some physiological indicators. Some of the shoot samples were wrapped in aluminum foil and stored in a freezer (-20 °C) to determine the amount of chlorophyll (type a, type b, and total), carotenoids, total phenol, vitamin C, and antioxidant activity. Oxalic acid, zinc, iron, copper, sodium, potassium, phosphorus, calcium, magnesium, and nitrate were determined in oven-dried samples. One-way ANOVA was applied separately to spring and fall data, and mean comparisons were made using Duncan's test at the 0.05% level. Principal component analysis was used to determine the relationships between nutrient elements and physiological indicators of spinach.

The LG-Ch-A and C500 treatments (in spring cultivation), and the LG-A-B, LG-All, and HG-All treatments (in fall cultivation) had the highest leaf number, leaf area, and yield and were significantly difference from the C0 treatment. The high yield in C500, LG-Ch-A, LG-All, and HG-All treatments was associated with nitrate accumulation in spinach. In both cultivations, there was a significant positive correlation between the amount of P, K, Mg and Zn in spinach and the amount of these elements in the corresponding composts. A synergistic relationship was also observed between P and Mg; P and Zn; and Mg and Zn in spinach. On the other hand, an antagonistic relationship was observed between Ca and Mg in spinach because a high concentration of calcium inhibits magnesium uptake by reducing cell permeability. In both seasons, the chemical fertilizer treatments showed the highest amount of chlorophyll and carotenoids because these compounds increase with increasing nitrogen availability. On the contrary, the amount of antioxidant activity was significantly higher in compost treatments than in chemical treatments. In the spring cultivation, the highest and lowest amount of oxalic acid and oxalic acid/Ca ratio were observed in the LG-Ch-B and HG-All treatments, respectively. Interactions between nutrients and physiological indicators were observed. The uptake of all micronutrients, P, and Mg (in both cultivations) and K (in the fall cultivation) was inhibited by high Ca concentration. With the decrease of micronutrients uptake, an increase in nitrate accumulation may occur because micronutrients are present in the structure of nitrate reducing enzymes. The interdependence between Mg and oxalic acid/Ca (in spring), K and oxalic acid (in fall), and Na and oxalic acid/Ca (in fall) may be related to the role of oxalates in the uptake of mineral ions by plants, since oxalates are usually combined with Na, Mg, Ca, and K in the form of soluble and insoluble salts.

The use of urea chemical fertilizer (at two levels) and agricultural waste composts had different effects on the physiological indicators, growth and nutrients in spinach. Spinach grown in soils treated with composts rich in P, K, Mg, and Zn had higher nutritional value. The grouping of treatments by principal component analysis showed that chemical and control treatments were clearly separated from compost treatments with high amount of chlorophyll, carotenoid, nitrate, K, and Zn and low amount of oxalic acid, oxalic acid/Ca ratio, antioxidant activity, phenol, and Na. In general, the use of C500, LG-Ch-A, LG-All and HG-All treatments is not recommended due to nitrate accumulation in spinach.

In the process of conducting this research, 110 studies on monitoring nitrate content in agricultural products produced in Iran were gathered. These studies were published until March 20, 2023. The collected studies underwent evaluation for methodology, quality control, and quality assurance of results and were then assessed based on standard research criteria. The evaluation of the study's methodology revealed that only 27.3% of the studies adhered to standard methods for sampling, storage, and sample transfer. In 15.5% of the studies, the method of preparation and extraction followed standard procedures. Additionally, in 30% of the studies, the nitrate measurement method was conducted according to established standard procedures. The evaluation of quality control in the studies showed that only 20.9% reported some indicators of quality control and assurance. The assessment of nitrate concentration in agricultural products revealed the weighted mean concentrations of nitrate (mg kg-1 fresh weight) as follows: potato 98.7, cucumber 120.9, tomato 40.7, onion 39.6, watermelon 19.9, melon 62.9, carrot 151.1, eggplant 235.2, peppers 329, other root and tuber vegetables 542.4, lettuce 907.7, spinach 995.4, celery 1093.4, fresh edible legumes 37.7, parsley 596.5, cabbages 414.7, other leafy vegetables 635.6, apple 32.4, and orange 37.2. The findings of the present research reveal that the mean nitrate concentrations in Iran’s agricultural products fall below the maximum permissible levels outlined by both the Iranian national standard and international standards. As a result, it can be generally concluded that there is no significant cause for concern regarding residual nitrate concentration in Iran’s agricultural products.

Indiscriminate use of nitrogen fertilizers causes the accumulation of nitrate in agricultural products, especially vegetables. The high concentration of nitrate in vegetables can threaten the health of society. The purpose of this study is to investigate the status of nitrate as one of the characteristics of product quality and to estimate the daily uptake and determine the hazard quotients (HQ) of the non-cancerous diseases. Sampling of soil and fruit from a greenhouse (cucumber and strawberry) and a field (cucumber and tomato) in Jiroft was done in 2019. Nitrate concentration was measured by the distillation method.The average concentration of nitrate in strawberries, tomatoes, greenhouse cucumbers and field cucumbers was 55.23, 16.86, 64.16 and 44.39 mg/kg of fresh weight, respectively which were less than the standard limit of nitrates based on the Russian and Iran national standards. The calculated daily uptake was lower than the permitted level (3.7 mg/kg BW). HQ in all products was less than 1, of which the lowest amount was found in strawberries for girls under 6 years old (0.00086) and the highest amount was found in greenhouse cucumbers for girls under 6 years old and for boys 7-14 years old (0.0615). Therefore, strawberry has the least and the greenhouse cucumber has the most health threat potential for the health of consumers. According to the risk index of less than 1 for all age groups, there is no possibility of encountering non-cancerous diseases caused by nitrates through the consumption of vegetables in Jiroft region.

Conventional cropping systems, dependent on heavy application of chemical fertilizers, are not ecologically and environmentally sustainable; they are a threat for soil and water quality and, in consequence, for plant and human health. Nitrogen fertilizers are heavily applied in conventional leaf vegetable production systems to obtain maximum growth and yield. However, the excess nitrogen tends to accumulate in leaf vegetables in the form of nitrate, which pose serious human health hazards. Therefore, to supply nitrogen from non-chemical sources, such as organic amendments, is a sustainable practice for production of leaf vegetables. Spent mushroom substrate (SMS), which is the remaining material after the harvest of mushroom, is produced in large quantities (5 kg SMS for 1 kg of mushroom) and is enriched with organic carbon, N, P, K, and micronutrients. Therefore its reuse as a soil amendment not only provides essential elements for plants but also improves soil quality. Similarly, incorporation of green manures, especially legume green manures, into cropping systems is a sustainable practice for soil fertility and soil quality management. In this study, we aimed to investigate the short-term effects of two soil organic amendments (spent mushroom substrate and alfalfa residues) and their combination, in comparison to inorganic N fertilizer (urea), on soil fertility, and selected essential nutrients, and nitrate accumulation in a leaf vegetable, test plant (spinach).    

A one-season pot experiment was led in a randomized complete block design with three replications in experimental greenhouse of Bu-Ali Sina University. Treatments were comprised of two levels of spent mushroom substrate (SMS-1: 2% SMS, and SMS-2: 5% SMS), two levels of alfalfa green manure (AGM-1: 1% AGM, and AGM-2: 3% AGM); two levels of the mixture of SMS and AGM (SMS+AGM-1: 1% SMS plus 0.5% AGM; and SMS+AGM-2: 2.5% SMS plus 1.5% AGM);  two levels of urea fertilizer (U-1; 120 kg/ha, and U-2: 360 kg/ ha); and control. Selected properties of the initial soil and both organic amendments were determined. Spinach (Spinacea oleracea L.) was seeded as leaf vegetable, test plant in early autumn 2017. After ten weeks, spinach were harvested and the aboveground and root dry weights were determined. Moreover, the content of NO3-, P, Fe, Cu, Zn, and Mn in edible parts were measured. Soil samples were analyzed for EC, pH, total organic carbon, available P and K, and alkaline phosphatase activity.

All soil quality indicators were significantly affected by the treatments. TOC was significantly increased in all of the organic treatments compared to the chemical and control treatments. The maximum increase in TOC was observed in SMS-2, SMS+AGM-2, and AGM-2 treatments, compared to the control (134, 130 and 107%, respectively). A decreasing trend in TOC was detected in the high level of urea treatment (U-2) compared to the control which can be explained by the faster decomposition of soil organic matter in the presence of higher inorganic N inputs. Both organic amendments (in both levels) and the higher level of urea (U-2) decreased soil pH compared to the control. The initial low pH of SMS (5.6) and AGM (6.2), in the first case, and oxidation of urea to nitrate, in the latter, may justify this observation. In contrast, soil EC increased under the both organic amendments relative to the control and U-1 treatments. Moreover, the adverse effect of SMS on soil salinity was greater than AGM due to the initial differences in their corresponding source materials (5.8 vs. 3.0 ds/m). Available K was significantly increased in the second level of all organic treatments compared to the chemical and control treatments. As for available P, all organic treatments, except AGM-1, led to the significantly higher P than the chemical and control treatments. It is reported that organic materials compete with mineral particles for P adsorption and increase its availability. Moreover, all organic treatments, except SMS-1, significantly increased phosphatase activity compared to the chemical and control treatments. This could contribute to the mineralization of organic materials and increase available P.   Spinach yield was affected by the experimental treatments. The highest increase in shoot dry weight occurred in SMS+AGM-2 and AGM-2 treatments by 235 and 230%, respectively, compared to the control. Moreover, the second level of all organic treatments as well as the first level of SMS plus AGM treatment significantly increased yield compared to the chemical treatments. Spinach P content was significantly higher in all organic treatments, except SMS-1 and AGM-1, compared to the chemical and control treatments. Organic amendments, by decreasing the surface adsorption of P and increasing soil microbial biomass, promote the availability of P for plants. Spinach nitrate content ranged from 265 (in control) to 7807 mg/kg (in U-2). According to the critical limit of nitrate in spinach (4000 mg/kg) presented by European Union, only U-2 treatment led to over-accumulation of NO3-. The two levels of AGM treatments and SMS+AGM-2 resulted in the comparable amounts of nitrate as the recommended amount of urea (U-1). A narrow variation in spinach Cu content (from 6.1 in SMS+AGM-2 to 9.8 mg/kg in AGM-2), all within the standard range reported for plants (5-20 mg/kg), was observed among the treatments. Spinach Fe content was increased under all organic treatments relative to the control, although some disparities were not significant. The lowest Fe was detected in U-2. It is reported that excessive N may diminish root growth and, in turn, reduce nutrient uptake. Spinach Zn content varied from 44.8 (in control) to 71.5 mg/kg (in SMS-2), which was close to the higher limit of standard range (20-50 mg/kg) reported for vegetables, but lower than toxic concentration range (200-400 mg/kg). Spinach Mn content varied from 17.4 (in control) to 32.1 mg/kg (in SMS-2), which was close to the lower limit of the standard range (40-400 mg/kg) reported for plants.

The most appropriate treatments in view of improving yield and soil quality (i.e., optimum TOC, P, and K; and lower EC) as well as tolerable nitrate accumulation were SMA+AGM-1 and SMS-1 in decreasing order. These treatments are preferred over the chemical treatments (U-1 and U-2).

Spinach (Spinacia oleracea L.) has been known as iron rich vegetable but in many cases it not only lacks enough iron but accumulates a high amount of nitrate due to irrigation with polluted water around the cities. It seems that Pseudomonas species could diminish nitrate accumulation by inducing nitrate reductase activity (NRA) and could increase iron uptake by producing siderophore. In a factorial experiment based on randomized complete block design with two factors and three replications, spinach plants were inoculated with bacteria (Pseudomons fluorescens Cha0, P.putida Tabriz and non-bacterial) and treated with three levels of nitrogen (0, 125 and 250 mg N Kg-1 as urea).Statistical analysis showed that with increasing levels of nitrogen in soil from 0 to 250 mg kg-1 nitrate concentration and iron was significantly increased in shoot while shoot nitrate reductase activity had not significant changes. By applying the bacteria, nitrate reductase activity was significantly increased in shoot and the most NRA about 180.51% was observed in plant inoculated with P.putida compared to the non non inoculated treatments. Nitrate concentration showed about 32.98% decrease P.putida compared to control. Also iron concentration, increased up to 40 and 26.85% in shoot in P.putida and P.fluorescens treated plants, respectively compared to the non-bacterial. Based on the result obtained in this study, The highest decrease in nitrate accumulation, the highest nitrate reductase activity and iron cocentration in spinach shoots were achieved by P.putida inoculation.

Overuse of nitrogen fertilizer may result in nitrate accumulation in vegetables. The objective of this study was to determine the effect of Ammonium nitrate fertilizer and foliar application of mixed amino acids on the yield and nitrate accumulation in radish (Raphanus sativus). The experiment was based on the randomized complete blocks design and was carried out in pots filled with 6 kg of agricultural soil in factorial form with three replications. Three levels of nitrogen fertilizer (ammonium nitrate), N1: 0 mg, N2: 45 mg, N3:90 mg per one kg of soil and three levels of mixed amino acids (provided by Inagrosa company, Spain) A1: 0, A2: 1.5 and A3: 3 ml per one liter of water were used. Based on the results, the interaction effects of N fertilizer and amino acid application were significant on the yield and nitrate concentration. The results showed that the highest yield was in treatment N3A3 (2489 gr per pot) compared with the other treatments. Nitrate concentration in leaves and glands was increased with increasing the amount of ammonium nitrate. The highest concentration of nitrate in leaves was in treatment N3A1 (431 mg kg-1FW) and in glands was in treatment N3A2 (1331 mg kg-1FW). In leaves, foliar application of amino acid increased the total N and decreased the nitrate concentration. Foliar application of amino acids affected the nitrate reductase and glutamine synthetase and decreased the nitrate accumulation in leaves but had no effect on nitrate concentration of glands.