جستجوی مقالات مرتبط با کلیدواژه « Enzyme activity » در نشریات گروه « اکولوژی »

This study aimed to investigate the effect of potassium solubilizing bacteria on quantitative and enzymatic characteristics of canola (Brassica napus L.) cultivar ‘Hayola 50’ under saline soil. 

The experiment was carried out based on a split-plot arrangement in a randomized complete block design with three replications. The studied treatments were included potassium sulfate fertilizer at seven levels control, 25, 50, 75, 100, 125 and 150 kg.ha-1 PSF as the main plot and the bacterial inoculations at five levels (control or non-bacterial inoculation, Pantoea agglomerans, Rahnella aquatilis, a combination of two bacteria Pantoea agglomerans and Rahnella aquatilis and a commercial potassium biofertilizer (containing Pseudomonas koreensis and Pseudomonas vancouverensis) as the sub-plots. At the end of the growing season, traits such as grain yield, biological yield, grain oil yield, the amount of sodium and potassium uptake in the canola shoot and grain and the activity of antioxidant enzymes such as catalase, peroxidase and superoxide dismutase were measured. 

The results indicated that most of the studied traits were affected by PSF and inoculation methods. Moreover, the interaction effects were statistically significant on grain yield, oil yield, SOD enzyme activity and sodium uptake in the stem. The co-inoculation with P. agglomerans and R. aquatilis as the best treatment caused a significant increase in the yield of grain, biological and oil, potassium and sodium uptake in grain and stem by 46.0, 33.9, 71.2, 21.0, 21.1, 16.1 and 18.9%, respectively, and also a significant decrease was observed in CAT, GPX and SOD enzymes activity by 26.7, 28.3 and 25.3%, respectively, compared to the control. According to the results of regression analysis, the highest biological yield was found when the co-inoculation with P. agglomerans and R. aquatilis was applied which was 39.0% higher than those obtained in the control condition. The maximum rate of increase in grain yield (8.38 kg) per kg increase in PSF belonged to the co-inoculation treatment which caused 51.06% higher grain yield in comparison with the control condition (3920 kg.ha-1). Besides, the highest oil grain yield was obtained when the co-inoculation treatment was used along with 100 kg.ha-1 PSF (73.9% more than the control condition). 

In general, a combination inoculation of these bacteria could increase the yield of grain and oil and also improve canola growth under saline soil conditions which can be considered as an appropriate method for reaching sustainable rapeseed cultivation.

In this study, larval midgut α-amylase activity of the Mediterranean flour moth reared on wheat flour under laboratory conditions was studied. Enzyme activity was evaluated by using a commercial kit and an autoanalayzer device. Specific enzyme activity was measured as international unit per mg protein (IU/mg Protein). In this study, some α-amylase enzyme properties such as optimum temperature and pH of enzyme activity, enzyme activity during different hours of the day and night, enzyme activity at different developmental stages of adult male and female, effect of starvation period, and the effect of storage conditions of enzyme at specified periods in two different temperatures were studied. The results revealed that incubation temperature significantly affected enzyme activity for both sexes and optimal enzyme activity was detected in 40˚C for both male and females. pH values also significantly affected enzyme activity and the optimal pHs for amylase activity in both sexes were 5. Effects of enzyme concentration on α-amylase activity were significant in both sexes. Increasing the concentration of enzyme in both sexes caused a sharp increase in enzyme activity. Storage period in -20˚C did not decreased enzyme activity significantly in both sexes, but storage period in 4˚C in males significantly decreased the activity in comparison with the control. The results of studying enzyme activity during 24 hr. periods showed significant differences during day and night and the most and the least enzyme activities in both sexes were recorded in 12 and 24 o’clock, respectively. Enzyme activity in different developmental stages showed significant differences and the most enzyme activities were detected in 5th female larvae, 5th male larvae, 4th male and females, female and male pre-pupa, and female and male adults, respectively. In male and female pupae, no detectable activity was observed.Enzyme activity in male and female fifth instar larvae showed Significant difference. Starvation periods significantly affected the digestive enzyme activity in both male and female larvae and the trends of enzyme activity during 4 days of starvation was the same in both sexes. The most and the least enzyme activity were detected 2 and 4 days after starvation, respectively.