جستجوی مقالات مرتبط با کلیدواژه « آلژینات سدیم » در نشریات گروه « باغبانی »

The extinction threatens some native orchids which has economic value. Artificial seed technology can prevent the extinction of these plants and can be suitable for producing and exporting of product. In this study, protocorms of Dactylorhiza umbrosa were encapsulated. The effects of three temperature treatments 4, 20, and 25°C were investigated during three periods 30, 60, and 90-days of artificial seed storage. Percentage of artificial seed germination during storage conditions, after storage, total percentage of germination and number of days required for germination incidence, were investigated. The highest germination percent during storage conditions was related to 90-days storage at 4 and 25 ° C, and the lowest obtained after 30 days at 4 ° C, which did not show any germination. The highest germination percentage of artificial seeds after storage and cultivation was observed in 60 days stored seeds at 4 °C. The highest total percentage of germination was obtained after 60 and 90 days storage at 20 and 4 °C, respectively. The highest number of days befor germination incidence was observed after 60 days of storage. The best treatment for storage of artificial seeds was 4 °C for 30 and 60 days, which showed the lowest percentage of germination during storage conditions and the highest percentage of germination after culturing.

This investigation was carried out to determine the conversion and growth ability of artificial seeds derived from encapsulation of sunflower shoot tips in different substrates as factorial based completely randomized design with five replications, in tissue culture laboratory of Kermanshah Agriculture Research Center in 2013. Shoot tips excised from in vitro proliferated sunflower plantlets were encapsulated in three percent sodium alginate and 100 mM CaCl2.2H2O. Determination the effect of using nutrients and plant growth regulators, alginate matrix supplement with three different matrixes include distilled water, liquid MS medium and liquid MS medium whit tow mg/l BA and tow mg/l IAA. Synthetic seeds were transferred on hormone-free MS medium and two commercial substrates mixture of cocopeat, perlite (1:1) or cocopeat, perlite and peat moss (1:1:1) for generation and growth ability evaluation. Using of MS nutrients and plant growth regulators in calcium alginate beads significantly improved encapsulated explants growth. Maximum percentage response for conversion of synthetic seeds was 100 percent that was achieved from in vitro culture on MS medium and directly culture in ex vitro condition caused to decrease the conversion and growth them.