جستجوی مقالات مرتبط با کلیدواژه "cytogenetic" در نشریات گروه "باغبانی"
تکرار جستجوی کلیدواژه «cytogenetic» در نشریات گروه «کشاورزی»-
آویشن از مهمترین گیاهان دارویی است که کاربردهای وسیعی در صنایع دارویی، غذایی، آرایشی و بهداشتی دارد. پژوهش های سیتوژنتیکی، نقش مهمی در تعیین قرابت گونه ها ایفا می کند و به عنوان اولین تجزیه فیلوژنی و تکامل گروه های خویشاوند مطرح است. تعداد، اندازه و شکل کروموزوم از فاکتورهای مهم در بررسی تکامل هستند. در اصلاح ژنتیکی هر گونه گیاهی داشتن اطلاعات کافی در خصوص سطح پلوییدی و ویژگی های کاریوتیپی از مهم ترین نیازهای اولیه اصلاح گران می باشد. در این مطالعه ویژگی های کاریوتیپی برخی گونه های آویشن متعلق به بخش Serpyllum و زیر بخش Kotschyani مورد مطالعه قرار گرفت. در این مطالعه از سلول های مریستم ریشه چه و در مرحله متافاز میتوز برای بررسی ویژگی های کروموزومی و کاریوتیپی استفاده شد. بر اساس نتایج، عدد پایه کروموزومی در اکثر گونه ها 15=x و در برخی گونه ها 14=x بود. دو سطح پلوییدی (دیپلویید و تتراپلویید) در میان گونه های مختلف مشاهده شد. کروموزوم ها در اکثر گونه ها، به صورت متاسانتریک و ساب متاسانتریک بودند. بر اساس تقارن درون کروموزمی (A1 و TF) برخی جمعیت های گونهThymus eriocalyx متقارن ترین و ابتدایی ترین کاریوتیپ و گونه های Thymus lancifolious وThymus fedtschenkoi نامتقارن ترین و کامل ترین کاریوتیپ را دارا بودند و بر اساس تقارن بین کروموزمی (A2) گونه Thymus eriocalyx دارای نامتقارن ترین کاریوتیپ بود. بر اساس جدول دوطرفه استیبنز اکثر جمعیت ها در کلاس 1A و 1B قرار گرفتند ولی در جمعیت های گونهT. lancifolious کلاس 2A و3B نیز مشاهده شد. نتایج حاصل از این مطالعه می تواند برای درک بهتر جایگاه تاکسونومیکی و برنامه های اصلاح و اهلی سازی مفید باشد.
کلید واژگان: آویشن, تاکسونومی, زیر بخش kotschyani, سیتوژنتیکIntroductionThe Genus Thymus is one of the most important genera of the Lamiaceae family. According to Jalas (1971), Thymus is divided into eight sections: Micantes, Mastichina, Piperella, Teucrioides, Pseudothymbra, Thymus, Hyphodromi, and Serpyllum. About 18 species exist in some areas of Iran and most of them belong to Serpyllum Section and kotschiani Subsection. Chromosomal information is an important key for taxonomy, phylogeny, evolution, genetics and breeding in thyme. Therefore, this work was carried out to study the cytogenetic characteristics of genus Thymus belonging to the Subsection Kotschyani for breeding purposes and taxonomy.
Materials and MethodsSeeds were collected from 5 wild populations and germinated on wet filter paper at 20 oC. One cm long roots were pretreated with 8-hydroxyquinolin and then washed in distilled water and fixed in carnoy solution for 24 hours. Hydrochloric acid (1N) was applied for 7 min to hydrolyze the roots. Staining was done by orcein for 24 h at room temperature. Stained roots squashed in one drop of 45% acetic acid and examined by a ZEISS Axiophot compound microscope. Cells in metaphase stage were photographed with a D450; Canon Inc. Japan digital camera. Ten well prepared metaphasic cells were selected and some chromosomal characteristics such as total chromosomal length (TL), long arm length (L), short arm length (S), the arm ratio (AR) [LA/SA], and centromeric index (CI), were measured using Micro Measure ver. 3.3 software. The following karyological parameters were determined: total chromosome form (TF%), intrachromosomal asymmetry index (A1) and interchromosomal asymmetry index (A2). Karyotypic characteristics have been determined using the symmetry classes of Stebbins (SC). Karyotype formula was determined from chromosome morphology based on centromere position in accordance with the classification of Levan.
Results and DiscussionThis study reports the chromosome number and meiotic behavior of 31 populations belonging to 8 species of Thymus Subsect. kotschyani from Iran. In most species of this subsection, the base number of chromosome x = 15 and two diploid and tetraploid ploidy levels with chromosome number of 2n = 30 and 60 were observed. Only in T. migricus species, the base number of chromosome x = 14 was reported with two levels of diploid and tetraploid ploidy and chromosome numbers of 2n= 28 and 56. The chromosomes were mostly metacentric (m) or sub-metacentric (sm) in all species Among the various species with basic number of x= 15, the highest percentage of TF and the lowest amount of A1 were observed in populations belonging to T. eriocalyx (T7) indicating that this species has the most symmetric karyotypes. The lowest percentage of TF and the highest amount of A1 are in population of T. lancifolious (27) and T. fedtschenkoi (T28) species; this shows that the species has the most asymmetric karyotype among the species of this subsection. The highest amount of A2 was observed in T. eriocalyx (T7) species, indicated the asymmetry between chromosomes and chromosomal length. The difference in the number of chromosomes, ploidy levels and karyotype asymmetry in the populations and different species may be related to different environmental conditions in their habitat, pollination system (cross pollination) or polyploidy (Aneuploidy) in this genus.
ConclusionThe results showed that basic chromosome number in most species is 15 (x= 15). Two ploidy levels (diploid and tetraploid) were observed among different species. Chromosomes in most species, were metacentric and sub-metacentric. Based on intra- chromosomal symmetry (A1 and TF) T. eriocalyx species (T7) has the most symmetric and most primitive karyotype and the species T. lancifolius (T27) and T. fedtschenkoi (T28) have the most complete and the most asymmetric karyotypes. Also based on inter- chromosomal symmetry, (A2) T. eriocalyx (T) species has the most asymmetric karyotype.
Keywords: Cytogenetic, Karyotype, Subsec. Kotschyani, taxonomy, Thymus spp -
سیکلامن متعلق به خانواده Myrsinacea است و دربرخی مناطق معتدل زیر درختان و بوته ها رشد می کند.بدین منظورپژوهش در مورد صفات مورفولژی،فیتوشیمی و سیتوژنتیکی از سال 1394تا 1399 انجام شد.در این مطالعه سیکلامن ها از سه استان شمالی ایران،گیلان،مازندران و گرگان در فصل زمستان و بهار جمع آوری شده و صفات مورفولژی آنها در محل ارزیابی شد که این صفات شامل 15 صفت کمی و 9صفت کیفی بود. مطابق نتایج حاصل از تجزیه واریانس، تنوع معنی داری میان صفات مطالعه شده مثل تعداد برگ،تعداد گل،رنگ گل،شکل گل و برگ و...وجود داشت که امکان گزینش گیاهانی با صفات مطلوب را فراهم می کندو نتیجه حاصل از تجزیه کلاستر،گیاهان مناطق مختلف رابه سه گروه مجزا تقسیم کرد و نشان داد گیاهان منطقه کردکوی از استان گلستان و همچنین گیاهان منطقه چالوس از استان مازندران متفاوت با سایر مناطق هستند. گیاهان مناطق نور، بهشهر، گرگان و سیاهکل با وجودی که از سه استان مجزا می باشند؛ اما بخاطر وجود شباهت هایی که از نظر مورفولوژی دارند در یک گروه قرار گرفتند. گروه اول شامل سیکلامن های مناطق قرنآباد، لاویج، دیلمان، پاسند و آبپری است. گیاهان این مناطق از نظر شکل حاشیه برگ کاملا شبیه بوده و تضرس کمی داشتند. همچنین از نظر داشتن گلبرگهای بلند و تعداد بذر کم نیز شباهت داشتند. گروه دوم سیکلامنهای منطقه کردکوی بود این گیاهان سوخ هایی بزرگ و سنگین، برگهای بزرگتر و عریضتر و همچنین گل های کوچکتری داشتند. سیکلامنهای منطقه کردکوی بیشترین تعداد بذر، قطر، طول و وزن سوخ، تعداد گل، تعداد برگ، طول و عرض برگ، سطح برگ، طول دمبرگ و دمگل و نیز قطر دمگل را به خود اختصاص دادند. گروه سوم شامل مناطق سینوا و شاهچشمه چالوس بود. این گروه بیشترین عرض گلبرگ اما کمترین مقدار قطر دمگل، طول، عرض و سطح برگ، تعداد گل و برگ، وزن، طول و قطر سوخ را داشتند.
کلید واژگان: سیکلامن وحشی, صفات مورفولژیکی, فیتوشیمیایی, سیتوژنتیکیThe cyclamen (Myrsinascea) as the flowers growing in some temperate regions, are of high ecological and botanical importance and endangered as well. Therefore, to provide an opportunity to identify and protect its species, Iranian native cyclamen was studied. Plants were collected (in winter and spring) from three northern provinces of Iran, i.e., Guilan, Mazandaran, and Golestan, and their morphological traits (14 quantitative and 10 qualitative traits) were assessed. Based on the analysis of variance, there were significant differences in some recorded traits, e.g., leaf number, flower number, flower color, and flower and leaf shape, which would allow selecting plants with more optimal traits. The cluster analysis divided the plants into three groups. Accordingly, the plants collected from the Shadan region in Golestan province and those collected from the Sinava and Shahsheshmeh regions in Chalus County, Mazandaran province differed from the plants collected from the other regions. The plants were collected from the Abpari and Lavij regions in Nur County, the Pasand region in Behshahr County, the Qarnabad region in Gorgan County, and the Deylaman region in Siahkal County were placed in the same group due to their similar morphology although they had been collected from three different provinces. The first group included the cyclamens of the Qarnabad region in Gorgan County, the Lavij region in Nur County, the Deylaman region in Siahkal County, the Pasand region in Behshahr County, and the Abpari region in Nur County. These plants had lowly toothed leaf margins, long petals, and few seeds. The second group included the cyclamens of the Shadan region in Kordkuy County. These plants had longer and heavier hypocotyls, larger and wider leaves, and smaller flowers. They showed the highest number of seeds, hypocotyl diameter, length, and weight, flower number, leaf length and width, leaf area, petiole and peduncle length, and peduncle diameter. The third group included the cyclamens of the Sinava and Shahsheshmeh regions in Chalus County. They had the highest petal width but the lowest peduncle diameter, leaf length, width, and area, flower and leaf number, and hypocotyl weight, length, and diameter. The cyclamens native to Iran are highly diverse in morphological traits and they need to be further investigated by cytogenetic, phytochemical, and molecular methods.
Keywords: cyclamen, Cytogenetic, Hypocotyl, Phytochemistry -
تنوع ژنتیکی 13 اکسشن از گونه Lolium multiflorum بر اساس خصوصیات کاریوتیپی مورد بررسی قرار گرفت. برای هر اکسشن، کاریوتیپ 5 سلول متافازی تهیه شد و صفات طول کل کروموزوم (TL) ، طول بازوی بلند (LA) ، طول بازوی کوتاه (SA) ، شاخص سانترومری (CI) و نسبت بازوها (AR) با استفاده از نرم افزار micromeasure محاسبه گردید. شش اکسشن دیپلوئید و هفت اکسشن تتراپلوئید بودند و تعداد کروموزم پایه برای کلیه اکسشن ها x=7 بود، همچنین تعداد ماهواره از سه تا شش، در بین اکسشن های دیپلوئید و تتراپلوئید متغیر بود. نتایج تجزیه واریانس (ANOVA) بر پایه طرح کاملا تصادفی (CRD) نشان داد که در بین اکسشن های مورد بررسی برای کلیه صفات کاریوتیپی اختلاف معنی داری در سطح یک درصد وجود دارد. دیاگرام حاصل از پراکنش اکسشن ها براساس شاخص های نامتقارن بودن درون کروموزومی (A1) و نامتقارن بودن بین کروموزومی (A2) و نتایج تجزیه کلاستر اکسشن ها به روش Ward نشان داد که اکسشن های مورد بررسی در سه گروه قرار گرفتند. نتایج حاصل از تجزیه به مولفه های اصلی (PCA) نشان داد که دو مولفه اول به ترتیب 647/48 و 726/35 درصد از کل واریانس موجود را بر اساس صفات کاریوتیپی در بین اکسشن ها بیان نمودند. در مجموع 373/84 درصد از تنوع موجود بین اکسشن های مورد مطالعه با دو مولفه اول بیان شد. دیاگرام پراکنش اکسشن ها بر اساس دو مولفه اول، اکسشن ها را در چهار گروه قرار داد.کلید واژگان: سیتوژنتیک, تنوع ژنتیکی, کاریوتیپ, Lolium multiflorumGenetic variations were studied for 13 accessions of Lolium multiflorum using cytogenetical traits. Karyotype was prepared for 5 metaphases cells of each accession and the traits of total length (TL), long arm (LA), short arm (SA), arm ratio (AR) and centromer index (CI) were determined by micromeasure software. Six accessions were diploid and seven accessions were tetraploid, the basic chromosome number was x=7, and also three and six satellites were observed for diploid and tetraploid accessions, respectively. The accessions had an asymmetry karyotype, and variations were significant (at 1% level of probability) between accessions for karyotypic characters based on analysis of variance (ANOVA). Results of mean comparison showed that the diploid accessions had higher mean chromosome length than tetraploid accessions. The results of cluster analysis by Ward's method based on the values of intrachromosomal asymmetry index (A1) and interchromosomal asymmetry index (A2) showed that the studied accessions were fallen in three groups. The tetraploid accessions had higher intrachromosomal asymmetry than the diploid accessions. Principal components analysis based on karyotypic traits for accessions showed the first two components captured 84.37% of the total variance. Principal component analysis for grouping accessions based on Scatter plot identified four distinctive groups.Keywords: Cytogenetic, Genetic variation, Karyotype, Lolium multiflorum
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In this study, the karyo-systematic studies on the Iranian wild almond species A. communis L., A. corduchoruom Bornm., A. trichamygdalus woronow, Amygdalus lycioides Var. horrida Spach were done by the karyological methods. The meristem cells of the root tip were used for these studies. In each species, ten suitable metaphase plates were chosen and photographed so that the morphology of the chromosomes was completely obvious. The standard karyotype was prepared for the species separately and the parameters of the chromosomes, including the total length of the chromosomes, long arm, short arm, arm ratio, and centromer index, were calculated. There was a significant difference between all of the species that can be employed to recognize the species. All of the studied species were diploid, and the numbers of the chromosomes was 2n=16. The base number of the chromosomes in all of the species was X=8. In Iran, the average size of chromosomes in species of this genus was 2.42 micrometer.. Also, there was a significant difference between all of the homologous chromosomes according to the measured cytological characters. The similarity and the difference between the species were evaluated on the basis of the cytological specificities. The domestic species of A. communis L. had the most similarity with the species of Amygdalus lycioides Var. horrida Spach, and the species of A. trichamygdalus woronow and A. corduchoruom Bornm, also had the most similarity with each other. Finally, the studied species were classified into two cytologically groups.
Keywords: Almond, Breeding, Cytogenetic, Interspecific hybridization, Prunus -
Potato is one of the important and strategic crops, which limiting factors such as diseases reduce its yield. The cytological studies of some of resistant and susceptible cultivars of potato to early blight disease, Alternarias spp. that is A. alternata and A. solani which are important in potato fields were considered. The cultivars were including: ‘Agria’, ‘Santé’, ‘Shepody’, ‘Milova’, ‘Boren’ and ‘Ramose’. Measured characters were the number of chromosomes, length of longest and shortest chromosomes, ratio of the longest to the shortest chromosome, the mean length of long arm to short arm of chromosomes, mean length of chromosomes and karyotypic symmetry in each cultivar. The results showed that the base chromosome number was 12 and 2n=4x=48 for the studied cultivars. The type of chromosomes in all studied cultivars were metacentric, sub metacentric and sub telocentric. Karyotipic analysis of cultivars using relative length of the shortest chromosome (S%), total karyotipic shape (TF%) and the difference in the range of relative chromosome length (DRL) was performed. All cultivars were relatively symmetric. Comparison of relative length of the shortest chromosome (S%) and the difference in the range of relative chromosome length (DRL) showed that the resistant ‘Ramose’ and ‘Boren’ cultivars, had the most symmetric karyotype, and the susceptible ones including, ‘Agria’ and ‘Santé’ had the least symmetric karyotype. The other cultivars including ‘Shepody’ and ‘Milova’ were located at intermediate state.Keywords: Esfahan, Potato, Cytogenetic, karyotype, Early blight, Mitosis
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