جستجوی مقالات مرتبط با کلیدواژه "withania coagulans (stocks) dunal" در نشریات گروه "باغبانی"

Withania coagulans (Stocks) Dunal from solanaceae family is a multi-purpose species that is distributed in Iran only in limited areas of natural habitats in Sistan and Baluchestan province. Different organs of this plant have antioxidant, antimicrobial, and anti-tumor biological effects. To investigate the effects of humic acid and titanium dioxide nanoparticles on the amount of phytochemical compounds and some growth parameters of this species, a pot factorial experiment was performed based on a randomized complete design with three replications. Experimental factors included humic acid at four levels (H0: 0, H1: 10, and H2: 30 g.kg-1 soil) and titanium dioxide nanoparticles at three levels (T0: 0, T1: 45, and T2: 90 mg.l-1). The results showed that the H2T2 treatment increased fresh weight, dry weight, number of lateral branches, height, phosphorus, potassium, and relative water content compared to the control, significantly. Increasing humic acid up to 30 g decreased the phenols content from 48.59 in H0 to 39.88 mg.g-1 fresh weight in H2. In T2, the phenols content increased by 2.37% compared to T0, which had a significant difference with the other treatments. Also, a similar trend was observed for the parameters like total flavonoids and proline. But increasing humic acid up to 30 g increased the amount of chlorophyll fluorescence by 70.79% compared to the control. Overall, the results indicated that the interaction of humic acid and titanium dioxide nanoparticles could improve the growth and plant yield parameters.

In recent years, W. coagulans (Stocks) Dunal (Fam. Solanaceae) has gained much attention, owing to the presence of a large number of steroidal lactones known as withanolides. Out of the several withanolides isolated from W. coagulans, the biological compound, withaferine A is pharmacologically important that due to the significant and specific therapeutic action in cancer, Parkinson and Alzheimer’s disease. The present study was, therefore, undertaken to make an assessment of withaferin A content in the roots of different accessions of W. coagulans and in in vitro cultures. The seeds of five wild accssesion (USB001-5) were sown in greenhous. The cell suspension cultures were initialized from leaf explants derived callus on Murashige and Skoog (MS) medium, supplemented with 30 gL-1 sucrose (w/v), 2.0mg/l 2,4-D and 0.5 mg/l kinetin (Kin). Adventitious roots were induced directly from leaf segments on half strength MS medium (0.8% agar) with 2 mgl-1 indole-3-butyricacid (IBA) and 30 gl-1 sucrose. The withaferin A content was evaluated by TLC and HPLC method using standard withaferin-A compound. The results showed the presence of withaferin A in all accessions (21.01-44.54 µg/g D.W). In addition, there was significant differences among accession (P

Withania coagulans (Stocks) Dunal (fam. Solanaceae) is a multipurpose medicinal plant، mainly distributed in southeastern parts of Sistan and Baluchestan province. The important medicinal properties of W. coagulans are attributed to the presence of steroidal lactones called withanolides. Among withanoloids، withaferin A is an important phytoconstituent showing antitumor، antiangiogenesis and apoptosis induction properties. This study was aimed to investigate the variability of phytochemical composition of Withania coagulans roots. Twenty accessions of W. coagulans roots، collected from different natural habits of Sistan and Baluchestan province، were used in the present study. Thewithaferin A was assayed through thin layer chromatography (TLC) method andphytochemical estimation was carried out by standard methodologies to detect the presence of secondary metabolites، like total phenol، flavonoids and anthocyanin. Our findings revealed that withaferin A was found in all accessions collected from different natural habits. The photochemical investigation showed the presence of flavonoids (5. 70-6. 50%)، anthocyanin (4. 51-9. 51µmol/g) and total phenol (14. 91-23. 7µgGAE/mgD. W)، varying significantly among the habitats (p<0. 05). In the present study، TLC analysis confirms the exsistance of withaferin A in all W. coagulans accessions as well as the potential of Sistan and Baluchestan natural habits to produce this valuable component. However، the root extracts of W. coagulans، growing in natural habitats of Iran، have higher phenolic content. Hence، it can be used as a source of natural components for the pharmaceutical and food industries.

Development and implementation of effective programs to conservation، reclamation and efficient utilization of valuable plant species seem to have a special importance. To achieve this goal، ecological characteristics of important plant species، forming natural vegetation، need to be detected and recognized. Withania coagulans (Stocks) Dunal، belonging to the Solanaceae family، has received much attention in recent years due to the presence of a large number of steroidal lactones، known as withanolides. One of the most important withanolides is withaferin A، having anticancer properties. To study the autecology of W. coagulans، its habitats in Sistan and Baluchestan region were identified and habit characteristics including climate، soil physical and chemical properties، altitude، slope gradient، aspect and phenological stage were investigated in several locations. Results showed that this plant is exclusively distributed in limited areas of the province. The climate of the study area is arid based on Demarton aridity index. Average annual rainfall is 124. 8 mm with a relative humidity of 32% and average temperature of 24. 2 C°. This species is mainly (45%) distributed at an altitude of 1200 to 1400 meters above sea level، in slopes، valleys، road edges and mainly in waterways and the bed of seasonal rivers and in all aspects particularly the South and a slope from zero to 74%. The soil texture of the study habits is predominantly sandy loam with a pH of 7. 5-7. 8، an EC of 0. 5-5. 2 ds/m and 8. 4-23. 1% lime. W. coagulans is a drought resistant species growing in arid climate conditions with an average annual rainfall of 150 mm. In general، essential measures need to be taken towards the conservation and distribution of this rare and valuable species.