جستجوی مقالات مرتبط با کلیدواژه "تحلیل تبارزائی" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Prune dwarf virus (PDV) is a significant pathogen in cherry trees (Prunus avium L.), causing serious damage to agricultural crops. In spring 2022, samples were collected from cherry orchards in Golmakan, Khorasan Razavi province. The collected samples showed symptoms of dwarfism, spotted fruits, and young shoots with short internodes compared to healthy plants. To detect PDV in these samples, a reverse transcription polymerase chain reaction assay was used with specific primers targeting the movement protein of the virus. The amplified 756-nucleotide fragment was purified and sequenced. The obtained sequence was compared to available sequences in the gene bank using the BLAST tool in NCBI, and the highest similarity was observed with the Bulgarian strain (99.7%, MT152176), while the lowest similarity was observed with the Netherlands strain (88.8%, HM021231). A phylogenetic tree based on the movement protein was constructed, and the PDV strains were classified into two distinct groups, and the Golmakan strain was placed within Group I on the phylogenetic tree. Further comparison of the movement protein sequences among the virus isolates showed that the amino acid sequences of the movement protein and the RNA-binding domain of the movement protein are highly conserved among PDV isolates. In this research, for the first time, the sequence of PDV from Iran was registered in the gene bank (OR541106), originating from a cherry tomato garden. The phylogenetic analysis of PDV was conducted, with a specific focus on the movement protein gene.