جستجوی مقالات مرتبط با کلیدواژه « جایگاه صفت کمی » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

The salinity stress after drought is the most important and the most common environmental stresses that limit crop production worldwide, including Iran. In order to determine the genomic regions of some physiological traits of "steptoe×morex" drived doubled haploid population of barley under normal and salinity stress conditions, an experiment was conducted at Research Farm, The experiment arranged in two randomized complete block design with two replication. Each plot included four rows with length of 3 m and 25 cm inter-rows distance. Triats such as chlorophyll content, chlorophyll fluorescence (Fo, Fv and Fv/Fm), relative water content (RWC), prolin content, water soluble carbohydrate (WSC) and cell membrane stability (CMS) were measured. QTL analysis was carried out using genetic linkage map derived from 327 molecular marker of RFLP and QTL cartographer software with composite interval mapping method. Combined analysis of varianc showed significant difference between the lines for all the studied traits. The maximum correlation was observed between Fo and Fv/Fm. In general, we found 43 QTLs (13, 16 and 14 QTLs for normal, salinity stress and mean of two conditions, respectively.) for the studied traits. Phenotypic variances that were explained by these QTLs changed from 9.06 to 30.28, respectively, for Fo (qFO 1m) and Fv/Fm (qFvFm 2.1m) in the mean of two conditions. LOD scores were ranged from 2.61 to 8.74, respectively, for the QTLs of chlorophyll content (qCHC1m) and Fv/Fm (qFvFm 2.1m) in mean of two conditions. QTL position 81.2 cM of chromosome 2H, controlling Fv/Fm, was quite stable. Therefore, it can be used in marker asisted selection.

In order to mapping QTLs of 9 phenological and morphological traits of "Seri M82×Babax" drived recombinant inbred line population of wheat, an experiment was conducted in two alpha lattice with two replications under normal and salt stress conditions in 2012. Days to heading, days to anthesis, plant height (cm), spike number, grain number in spike, biological yield, thousand grain weight, harvest index and grain yield were measured. QTL analysis was conducted by composite interval mapping method separately for each trait in each condition and mean of two conditions. Based on combined analysis of variance, the main effect of genotype was high significant for all studied traits and transgressive segregation in both directions (positive and negative) was observed. Twenty seven QTLs were found for the studied traits. Phenotypic variation that were explained by these QTLs, varied from 5-16%. The highest and lowest phenotypic variations were related to days to anthesis and thousand grain weight. Highest and loest LOD scores were obtained for the QTLs of harvest index in mean of two conditions and thousand grain weight in salt condition. Most of mapped QTLs were unstuble. Therefore, use of MAS in this population for studied traits seen to be unefficient.