جستجوی مقالات مرتبط با کلیدواژه "داوودی" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Chrysanthemum (Chrysanthemum morifolium) is one of the most important ornamental plants which plays a significant role in the development of gardening industry in the world. The knowledge of genetic diversity is one of the prerequisite criteria for Chrysanthemum breeding with important economic goals. Molecular markers have a significant share in elucidation of inter and intra species genetic diversity. To this end, genetic diversity of a number of Iranian cultivars was molecularly investigated by sequencing a part of rDNA, using ITS4 and ITS5 primers. Genetic distance between Chrysanthemum cultivars ranged from 0.05 to 10.15, demonstrating the power of ITS region in revealing the genetic diversity among cultivars of morifolium, suggesting Iranian cultivars have been genetically improved from morifolium species. Genetic diversity assessment of Iranian Chrysanthemum cultivars demonstrated that presumably inter, intra species or even inter population hybridization may have been involved in creating enormous genetic diversity among Chrysanthemum cultivars.

Physiological activity and biochemical changes continues in postharvest of cut flowers. These conditions reduce the longevity of flowers and accelerate the senescence process. In order to identify informative AFLP markers for description of senescence in Chrysanthemum, association analysis was used. The relationships between biochemical traits and molecular markers based on 25 primer combinations (EcoRI and MseI) and genetic structure of 44 chrysanthemum genotypes were evaluated. The results of correlation coefficient indicated a positive and significant correlation between protein concentration and lipid peroxidation (r=0.304**) as indicators of senescence. Results of MLM association analysis model for 5 variable showed that 143 AFLP markers were found to be associated with biochemical traits in Chrysanthemum, whereas GLM model identified 419 markers for biochemical traits. Moreover, we were able to identify 49 markers associated with malondialdehyde. However, only 14 AFLP markers associated with petal senescence. The strongest association was detected between AFLP markers of M-CAC/E-AGA13, with superoxide dismutase enzyme which explained 47 percent of variation. The most variation of petal senescence (32%) was accounted by M-CTT/E-AAC35 marker. Also, our results showed that some of the markers were co-associated with different chemical traits. Moreover, informative markers such as M-CTG/E-ACC16, M-CAA/E-AAG40 and M-CTG/E-AGA3 that have been shown to significant correlation with several traits can used for breeding programs and other analyses associated with future studies of Chrysanthemum if they contribute to increase longevity in other experiments.