جستجوی مقالات مرتبط با کلیدواژه "فسفیت پتاسیم" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Pythium ultimum is one of the major causative agents responsible for damping off disease in cucumber plants. In the present study, the effect of potassium phosphite (KPhi) on defense response of P. ultimum-inoculated cucumber plants was investigated. Different plant growth parameters as well as chlorophyll a content were studied to evaluate the healing effects of KPhi. Furthermore, the expression pattern changes of a pathogenesis-related chitinase gene was analyzed via qPCR. Results revealed that KPhi treatment significantly increased growth parameters i.e. shoot length, diameter and mean leaf number in cucumber seedlings. KPhi treatment at 1 and 4 gL-1 caused 31.37% and 94.48% increase in shoot diameter respectively compared to control plants while shoot length of plant treated with 1 and 4 gL-1 KPhi were increased 72.14% and 78.85%, respectively compared to control plants. The chlorophyll a content as well as plant leaf number was significantly increased in plants treated with 1 or 4 gL-1 KPhi compared to control plants. It was interestingly revealed that KPhi application decreased Chitinase gene expression compared to control plants. The findings of the present study would be implemented for designing a controlling strategy to decrease the adverse effect of P. ultimum on cucumber plants.

Phytophthora species are considered as the major cause of several plant diseases resulting in huge yield losses in agricultural crops. Despite years of effort to develop Phytophthora resistance varieties, there is no reports of a resistant cucumber variety. In this study, the effect of concomitant application of potassium phosphite (KPhi) and chitosan on some physiological and molecular responses of Phytophthora capsici-challenged cucumber plants were investigated. Cucumber plants were treated with KPhi and/or Chitosan at different concentrations and were then inoculated with zoospores of P. capsici and leaf samples were collected at different time courses. Results showed that Guaiacol peroxidase (GPOD) enzymatic activity surged immediately at first and second days after pathogen inoculation with a peak in plants treated with 4 gL-1 KPhi 2 days after inoculation. Compared to GPOD, the highest superoxide dismutase (SOD) activity was observed in the same treatment but later at 5 days after inoculation. It was indicated that the activity of antioxidant enzymes was greatly influenced by application of either KPhi or chitosan while their activity was not remarkably enhanced in control plants. qPCR analysis revealed that the highest increase in glutathione peroxidase (gpx) gene expression was achieved in plants concomitantly treated with 4 gL-1 KPhi and 200 mgL-1 chitosan 5 days after inoculation. The findings of this study provide novel information regarding inducing mechanisms of KPhi and chitosan which may be effective in mitigating disease severity.