جستجوی مقالات مرتبط با کلیدواژه "ژنتیک جمعیت" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

This study was conducted to investigate intra- and inter-population genetic diversity of 15 populations of Pistacia atlantica from Zagros forests, represented by 181 genotypes, using 15, 12 and 13 primers based on three molecular marker including inter simple sequence repeats (ISSR), inter retrotransposal amplified polymorphism (IRAP) and start codon targeted (SCoT) markers, respectively. ISSR, IRAP and SCoT primers produced a total of 186, 143 and 136 bands, of which 162 (87.0%), 130 (90.9%) and 120 (88.0%) showed polymorphisom, respectively. Results showed that all marker systems revealed relatively high genetic variation at population level (hISSR = 0.22, PPLISSR = 61.92%; hIRAP = 0.25, PPLIRAP = 74.19%; hSCoT = 0.20, PPLSCoT = 64.09%). According to ISSR, IRAP and SCoT based genetic differentiation coefficient (ΦST; 0.28, 0.23, and 0.22, respectively), a higher level of genetic variation was recorded within populations than that of among populations, that could be due to wind-based extensive gene flow among the populations. UPGMA cluster analysis based on three used molecular systems, devided the studied populations into distinct clusters. According to Mantel test, no significant correlation was recorded between genetic and geographic distances of the populations.

Woody vegetative coverage of Zagros forests is mainly composed by oaks. Information on natural patterns of genetic variation is of fundamental practical importance for sustainable forest conservation and management. In the present study, to assess the genetic differentiation and population diversity of eight Quercus brantii populations with 104 genotypes from Ilam forests, genomic DNA was extracted and then amplified fragments from different primers were visualized and scored on 1.2% agarose gel. Of 22 screened ISSR primers, 15 primers produced repeatable and scorable bands. Dice and Jaccard similarity matrix were analysed. Then the data were molecularly analyzed using molecular analysis software, GenAlEx. The selected primers amplified 189 bands, from which all were polymorphic. The number of amplified fragments varied from 7 to 20 with an average of 12.6 bands per primer, and with size range of 100 to 1800 bp. Analysis of molecular variance (AMOVA) showed that major proportion of total genetic variation (79%) was attributed to within populations, and the remaining 21% was resulted from among population variation. High level of genetic variation was observed within Q. brantii natural populations from Ilam forests.

Inter- and intra-population diversity of nine brant oak (Quercus brantii) populations، comprising of 125 genotypes from north-Zagros forests was investigated using start codon targeted (SCoT) molecular marker، and seed and leaf biochemical characteristics. Variance analysis of 12 biochemical properties disclosed significant inter-population differences for most of the estimated parameters. Principal component analysis (PCA) showed that the first three components contributed in 63% (seed) and 71% (leaf) of revealed phenotypic diversity. Cluster analysis of phenotypic data grouped the studied populations into three distinct main clusters. Ten SCoT primers amplified 118 amplicons، of which 113 products (95%) were polymorphic. The primers revealed a high genetic diversity at species level (He =0. 231; PPL% = 95. 7%). Analysis of molecular variance attributed only 23% of total revealed genetic diversity to intra-population diversity. Low-level genetic differentiation of populations seemed to be due to extensive gene flow by wind-pollination of the populations، which occurs naturally on oaks. SCoT markers-based cluster analysis divided the Q. brantii’s populations into distinct groups. According to Mantel’s test، there was no significant correlation between genetic and geographic distances among the studied populations. In conclusion، this study indicated that both phonotypic and molecular markers could be useful to conduct studies when investigating the geographic pattern of genetic diversity of oak natural populations.

There is growing concerns of over utilization of fish stocks in the world so that marine biodiversity is under extreme pressure by commercial fishing activities. As a solution, molecular genetic studies are being applied to determine genetic characteristics and also to select desirable genes or gene combinations in commercially important species. Silver pomfret (Pampus argenteus) is one of the most important marine food fish species in Persian Gulf and Oman Sea. In this study, genetic variability and population/s structure of Silver Pomfret were studied by genotyping 144 individuals from 5 localities (Sistan and Blauchestan, Hormozgan, Busher and Khuezstan provinces) using 11 microsatellite loci. Results of Fst (0.040) and Rst (0.129) showed low genetic differentiation among regions examined. Analysis of molecular variance (AMOVA) revealed 4% of genetic variation between groups and high genetic diversity observed within groups. This study suggests allelic richness and high genetic diversity of species across Persian Gulf and Oman Sea. Therefore, using microsatellite loci can provide scientific data of genetic resources of this commercial important species in the area.