جستجوی مقالات مرتبط با کلیدواژه "allele" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

In this study, 44 accessions and cultivars of three species of Pistacia including P. atlantica (15 accessions), P. khinjuk (23 accessions), and P. vera (6 cultivars) collected from different parts of Ilam and Semnan provinces, Iran, were evaluated for leaf and fruit traits. Investigation of morphometric traits revealed that there was a high variability (CVph) in fruit traits compared to leaf traits among species. The results of the principle component analysis (PCA) revealed that the three main components explained 94.04% of the total variation among the accessions. In PCA1, the fruit weight, length, and diameter, kernel and shell weight were predominant in the first component and explained 52% of the total variation. According to the results of cluster analysis, the accessions were divided into three main groups so that, those belonging to each species were placed into separate groups. Also, in the second part of the experiment, genetic diversity among samples was investigated using 12 RAPD primers. A total of 71 alleles were identified and their sizes ranged from 280 to 2500 bp. The number of observed alleles for each locus ranged from 4 (OPA-18 and OPA-16) to 9 (Custom primer) alleles, with an average of 5.91 alleles per locus. The polymorphic index content (PIC) was the highest (0.81) for the OPB-14 locus and the lowest (0.089) for the OPA-13 locus, and was 0.35 among all RAPD loci. The Jaccard’s genetic similarity coefficient ranged from 0.27 to 0.84 among the samples. The obtained results of the current study will help in the classification, preservation, and identification of Pistachio genetic resources and can be effective in breeding improved varieties of this crop.

The common fig (Ficus carica L.), one of the most important fruit species, belongs to Moraceae family and is widely distributed in Iran. In this study, genetic variations among some genotypes of common fig collected from six different regions of Ilam province (Iran) were evaluated based on RAPD and ISSR markers. A total of 73 and 29 alleles were produced by 14 RAPD (with their sizes ranging from 350 to 2500 bp) and 5 ISSR (with their sizes ranging from 150 to 1500 bp) primers, respectively. The number of observed alleles for RAPD primers ranged from 1 (OPA-03) to 9 (OPA-09 and UBC-429), with an average of 5.21 alleles per locus. Also, the number of observed alleles for ISSR primers ranged from 3 (UBC-807) to 8 (UBC-810 and UBC-414), with an average of 5.8 alleles per locus. The highest and lowest values of Shannon's information index (I) was observed in the UBC-429 (2.18) and OPA-03 (0.12) primers, respectively. The Jaccard's genetic similarity coefficient ranged from 0.12 to 0.73 among genotypes based on RAPD data, while for ISSR it was recorded from 0.07 to 1. Also, based on RAPD and ISSR data at a similarity coefficient of 0.45, the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis divided the genotypes into six major groups. As a conclusion, there is a high genetic variability among fig genotypes, which is an important consideration for classification, utilization of germplasm resources and breeding programs of fig.

Acidic soils are a serious threat to the production of crops throughout the world. In order to study the alleles associated with soil acidity tolerance in barley plant, and also accomplish effective haplotype groups, phenotypic testing in pots containing acidic soil and in the form of Augment with 96 genotypes and 4 controls, and 27 traits were evaluated on plants. To investigate the allelic and haplotypic diversity, the genotypes were identified by 7 microsatellite markers related to soil acidity tolerance. Analysis of allelic variation showed that the highest pic and Gene Diversity were 3.429, 0.441 and 0.490 respectively, which belonged to the HvMATE-21indel marker, and the Cit7 marker had the lowest value. Also, the results of the haplotype study showed 41 haplotype groups. The group 16, which included genotype 6, had the highest yield and soil acidity resistance with a yield of 2.017 gr / plant. association analysis between molecular and phenotypic data suggested that among 20 effective alleles on the evaluated traits, the Do-D allele had the greatest impact on yield and its components with effect on 3 traits, number of seeds per spike, number of Fertile spike and yield (in plant). Do-B was also R2 equal to 39.5 for the number of spikes per plant of the highest R2 among the alleles involved in yields and yield components. The markers associated with tolerant haplotypes and tolerant genotypes in this study can be used in research and breeding programs.