جستجوی مقالات مرتبط با کلیدواژه "benzyladenine" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

One of the main objectives of table grape breeding programs is producing new seedless hybrids with large, firm, high flavor berries and consistent with consumers taste. Abortion of zygotic embryo in seedless grapes largely limits the efficiency of seedless cultivars breeding. Since cytokinins are known to increase the sink strength of seeds for assimilates, the present investigation was conducted to study the influence of pre-bloom sprays of benzyladenine (BA) on ovule development and embryo rescue of two stenospermic grape cultivars (Askari and White Seedless).Concentrations of BA were 0, 60 and 100 ppm. Ovules of White Seedless, 20 days and Askari 40 days after flower opening were dissected out of berries and then cultured in Nitsch & Nitsch medium containing 1μM GA3, 1μM NAA, 2 g/l activated charcoal, 20 g/l sucrose and 8 g/l agar. Evaluated characteristics were ovule browning, callusing and germination. BA Spraying had no significant effect on ovules blacking but percentage of browned ovules were significant between examined cultivars and the highest ovule browning were observed in White Seedless cultivar. Effect of BA sprays whit 60 and 100 ppm concentrations were significant on callusing and ovules germination. Results showed the significant effect of cultivars on the percentage of germination ovules. Ovules germination was 23.71% in Askari cultivar and 14.44% in White Seedless. Finally pre-bloom sprays of benzyladenine (BA) increase successfully of embryo rescue technique in Askari and Bidaneh sefid Cultivars.

Micropropagation of strawberry provides possibility of fast multiplication. Growth regulators can be effective on micropropagation of strawberry and fast multiplication. To attention to the importance of proliferation stage of micropropagation, in this research an investigation carried out on the interaction effect of benzyladenine (BA) and chitosan. The Murashige and Skoog (MS) culture medium was supplemented with BA in two concentration (1 and 2 mg/l) and Chitosan with high molecular weight in five concentration (0,20,30,40 and 60 mg/l). A factorial experiment based on completely de +sign with eight replication was used. Evaluated characteristics included number of shoots, diameter of shoots, length of shoots, number of leaves, size of leaves, chlorophyll content and dry weight of plant mass. Based on the obtained results there was not significant differences between 1or 2 mg/l concentration of BA from the point of effect on examined characteristics. The highest number of shoots, diameter of shoots, number of leaves and dry weight of plant mass were obtained in 1 or 2 mg/l BA and without chitosan. But the highest length of shoot and chlorophyll content were observed in combination of 1 or 2 mg/l BA with30 mg/l chitosan. The highest leaf size were obtained in combination of 1 or 2 mg/l BA with 0, 20, 30 or 40 mg/l chitosan. According to the results, 1mg/l BA was significant effective on proliferation of Selva strawberry.