جستجوی مقالات مرتبط با کلیدواژه "biomass" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Black poplar (Populus nigra L.) is one of the dioecious species that is planted in a large area for forestry and wood production in different regions of Iran, but there is less information about its sexual response to water scarcity. In the present study, this plant was used as a model species against water deficit stress, and some morphological and physiological responses of a male clone (62/127) and a female clone (62/154) of black poplar were investigated. A factorial experiment was conducted based on completely random design with four replications. There were four irrigation levels (100, 75, 50, and 25% of field capacity) and two gender or sexes (male and female). Seedlings were irrigated for 120 days in a pot experiment. The results showed that the effect of drought and gender (sex) was significant on many of investigated traits. The sex × irrigation regime interaction effect was significant for height growth, total biomass, stomatal conductance, and transpiration. In all the drought treatments, males plants had higher values of many traits than that for females. It was concluded that that, in the studied black poplar clones, males black poplar were less sensitive to water deficit stress than that for females.

Hairy and adventitious roots are efficient systems for expressing recombinant proteins. In the present study, the amount of DrsB1-CBDAvr4 recombinant protein in hairy and adventitious root systems was compared. To this end, the effect of different factors on the optimization of culture conditions to obtain adventitious and hairy roots was evaluated in three separate experiments by assessment of biomass production in T1 transgenic plants expressing DrsB1-CBDAvr4 recombinant protein. The efficacy of Agrobacterium rhizogenesis in producing hairy roots was ensured using rolC gene specific primers. The insertion of DrsB1-CBDAvr4 recombinant peptide transgene in the genome of hairy and adventitious roots was confirmed by PCR analysis. Also, the level of DrsB1-CBDAvr4 protein was measured in hairy and adventitious roots by ELISA analysis. Analysis of the variance of data showed that the highest number of roots and the longest roots were obtained in MS media supplemented with 1 mg/L NAA and 0.5 mg/L IBA. The results of adventitious root biomass showed that liquid MS medium containing 1 mg/L of NAA hormone had a significant effect (P <0.01) on biomass production. More biomass was obtained in MS medium supplemented with 1mg/L NAA, whereas a lower fresh and dry weight was obtained in a 1/4 MS medium with no NAA. The results also showed that ATCC15834 strain with MS media supplemented with 3% sucrose, 10 minutes inoculation time was high efficiency to induce hairy roots in tobacco plants. The results of ELISA analysis showed that clones obtained from both roots showed a significant difference in terms of total protein content. The amount of recombinant protein from hairy roots was much higher than that of adventitious roots.

Naphthalene is one of the main polycyclic aromatic hydrocarbons that is increasing in the petrochemical industry and is on the list of toxic pollutants. These contaminations are mutagenic and have a large impact on the ecosystem of the region, gradually they are emerging to the food cycle and human societies. Thus threaten human health, plants, animals, rivers, underground water and agricultural production. Germination is the first and most important stage in plant development. In this study to evaluate the effect of naphtalene (30 & 50 mg/L) on germination, radical growth and biomass of seedlings in Helianthus annus L., an experiment was conducted. Seeds germinated in petri dishes and seven days later, those were studied. The results showed that naphthalene was not have a significant effect on germination percent. It Reduced the radicle length and also reduced the dry and fresh weight of the seedlings. Therefore, naphthalene because of its non-polar and hydrophilic structure decreases the rate of water absorption and affects the germination rate, and as a result, leads to decrease in length of the radicle and biomass of the seedlings.

The lake of Sanandaj dam is located within the territory of Sanandaj city,in the longitude coordinates of 46° 33' and the northern width of 55° 20', in the upstream of Banidar village and over Komasi river. The field activity of this research was seasonal and was carried out for one year from June 2015 to June 2016. Considering the size of the lake, five stations were set up in the lake. Phytoplankton was sampled from three surface layers, the middle layer, light absorbing layer and the layer near the bed of the lake.Phytoplankton specimens were immediately recorded with 4% formalin fixation (fixed) and then examined and identified under microscope with a magnification of 10X, 20X and 40X. The results of the study on the composition and species diversity of phytoplankton showed that 54 species existed in the lake. In this study, six groups of phytoplankton including: Bacillariophyta, Chrysophyta, Pyrophyta, Cyanophyta, Chlorophyta and Euglenophyta with 20, 1, 9, 6, 16, and 2 species were identified. The seasonal analysis of species diversity of phytoplankton revealed that in spring, summer, autumn and winter, respectively, 23, 25, 25 and 18 species were recorded.The highest species variation belonged to summer and autumn with 25 species. In addition, exploring the demographic changes of 6 phytoplankton groups in the lake indicated thatthe highest and lowest densities of phytoplankton respectively with an average of 3750000 ± 239793 and 127481 ± 90656 belonged to autumn and summer.

To evaluate of salinity tolerance indices in 17 commercial cultivars and 34 lines of bread wheat against salinity of sodium NaCl، an experiment was conducted in growing season of 2012 in factorial experiment based on randomized complete block with two replications and in salt concentration of 0، 8 and 16 ds m in the greenhouse. In this study، total plant dry weight was measured. To assess the salt tolerance of genotypes stress susceptibility (SSI)، tolerance (TOL)، stress tolerance (STI)، geometric mean productivity (GMP) and the mean productivity (MP) indices were used. In this study، regarding to the indices of MP، GMP and STI، Farahan 4452، Saruq 4468، Trfan 4678، Parsi، Rezaieh 2745 and Nishapur genotypes identified to have the highest yield in both control and salt stress conditions. Therefore، they can be used in saline and non-saline or uncertain areas، also because they had high biological yield. On the other hand، it was observed that Roshan، MV-17، 22762، Zarea، 22919، Azerbaijan 2664 and Mahabad 1856 genotypes had the lowest rate of TOL and SSI indices and they can be grown in saline areas because of having high biological yield in stress condition.

Nitrogen and phosphorus are essential nutrients in crop production. In order to investigate the effects of different nitrogen and phosphorus levels on biomass and harvest index of mungbean, Partow variety a field experiment was conducted at the Research Farm of the Islamic Azad University of Shahre-rey in Tehran, Iran in 2009. The Experiment was laid out with factorial arrangement in a Randomized Complete Block Design with three replications. Five levels of nitrogen (0, 30, 60, 90 and 120 kg N ha-1) and six levels of phosphorus (0, 30, 60, 90, 120 and 150 kg P2O5 ha-1) were the treatment variables. Results showed that application of N and P fertilizers significantly increased the biomass and harvest index. Interaction effect of nitrogen and phosphorus was significant. With the increase of phosphorus application, nitrogen had positive effect on increase of biomass and harvest index. The maximum biomass (793.8 g m-2) was obtained when 120 kg N ha-1 and 150 kg P2O5 ha-1 were applied. The highest harvest index (28.62%) was recorded when 90 kg N ha-1 and 120 kg P2o5 ha-1 were applied.

Effects of different tillage systems and nitrogen fertilizer levels on forage production and some agronomic traits of silage maize (Zea mays L.) cv. KSC704 were studied. A split plot experiment based on a randomized complete block design with three replications was conducted. Three procedures of tillage (moldboard plow with disc, sweep with disc and double discs) and three nitrogen levels (100, 200 and 300 kg.ha-1) were applied. Plant height, leaf number, fresh weight, ear length and diameter, dry weight and forage production were measured. The effect of nitrogen levels on height, fresh weight, dry weight and silage production were significant. The maximum forage yield (51575.3 kg.ha-1) was obtained by 300 kg.ha-1 nitrogen application. The effect of tillage procedures on fresh weight, dry weight and forage production of silage were also significant. The maximum forage yield (49911.9 kg.ha-1) was obtained for the sweep along with disc treatment. The effect of nitrogen levels and tillage systems on fresh weight and forage yield of silage maize were also significant. The maximum forage production (53582.3 kg.ha-1) was obtained by double discs with an application of 300 kg.ha-1 nitrogen.