جستجوی مقالات مرتبط با کلیدواژه « cellular toxicity » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

The objective of the present study was to identify volatile compounds and investigate the antioxidant, antimicrobial and anticancer effects of Tribulus longipetalus Viv.
In this research, the Tribulus longipetalus Viv, which is an annual herbaceous plant, has many branches, lies on the ground, and has stems with soft hairs. The geographical spread of this plant is in the long sand dunes of Aran and Bidgol deserts. Biological properties, including free radical scavenging activity, total amount of phenolic compounds, antimicrobial properties and cytotoxic properties were investigated. Since the whole plant extract showed a high level of inhibition in the saltwater shrimp cell toxicity test (nearly 70 μg/liter), in order to obtain the effective components, solvent extraction using a decanter funnel was on the agenda. The three obtained components of hexane, ethyl acetate and water were analyzed separately. Also, by using a polystyrene divinylbenzene chromatography column, the effective component with higher inhibition ability was isolated. Among the effective components, except ethyl acetate, it had a relatively high inhibitory power, which was identified with the help of Hplc device.
Comparing the antimicrobial power of Tribulus longipetalusViv with antibiotics, showed that this plant has good antimicrobial properties. Also, considering that LC50 was determined as 70 μg/ml. This value indicates the good cytotoxic power of Tribulus longipetalus Viv.

This study investigated the effects of genetic damage, cell contamination and apoptosis, nanoparticle silver (nano-Ag), in vitro conditions by using fibroblast cell line rainbow trout gonad (RTG-2 cell line) as a model. Experiment was done with 9 treatments of different concentrations of silver nanoparticles (NanoCid) with average size of 10 nm, containing 0 (control group), 0.01, 0.05, 0.1, 0.5, 1, 5, 10 and 50 μg/ml. Cells exposed to the desired concentration of silver nanoparticles, and after 24 hours, were measured of toxicity with the cytotoxicity test and the effects of genetic damage tests micronucleus assay. The effect of silver nanoparticles on the growth and cell morphology also were evaluated at times 24, 48 and 72 hours after being exposed to silver nanoparticles. Results showed that silver nanoparticles have strong effects on genetic damage, cell contamination and Apoptosis on celles fish and this effects are concentration-dependent. The results of cytotoxicity test showed that the concentration of nanoparticles increases the cytotoxicity effects at concentrations of 50 μg / ml so that all cells were destroyed. Also results indicated an increase in the abundance of micronucleolus in all treatments in compared with control group, So that treatment 1 μg / ml with 232 % (P<0.05), treated 5 μg / ml with 251 % and treated 10 μg / ml with 261 % increase in frequency of micronucleolus showed significant differences in compared with control group. In general, it can be concluded that due to widespread production and consumption of products containing silver nanoparticles, the release of nanoparticles in the aqueous environment, is not far-fetched. Therefore, studying environmental impact of releasing nanoparticles and strategies to prevent or reduce these effects is required.