جستجوی مقالات مرتبط با کلیدواژه « glutathione » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Β-lactoglobulin (BLG) is a member of lipocalin superfamily. BLG structure has 2 intramolecular disulfide bonds and one free cysteine in cys121. BLG is one of the major bovine milk allergen that disulfide bonds are responsible for its low digestibility. It seems that the digestibility of BLG will be increased via the reduction of disulfide bonds. In the present work, we aim to study the effect of plant glutathione/thioredoxin systems on the digestibility of milk BLG. Thioredoxins (Trxs) are small and abundant disulfide reductase in all organisms. That themselves are reduced with Trx reductase or glutathione reductase or glutathione. Previously the genes encoding plant Trx, OsTrx20 was cloned and heterologously expressed in E.coli. In this work this protein was produced and purified in considerable amounts. The interaction of Trx with glutathione (GSH) was confirmed by insulin assay. The result of this assay showed that GSH was able to reduce OsTrx20. Therefore, in this work the effect of GSH/OsTrx20 on the digestibility of BLG was studied. To this end BLG was pre-treated by GSH/OsTrx20 at 4, 25 and 37°C and then digested with trypsin. The digestibility was studied by using SDS-PAGE and HPLC and allergenicity was studied by ELISA. The results revealed that GSH/Trx system significantly effect on the BLG digestibility and allergenicity.

In order to design a precise diagnostic and quantitative system to detect of P. betae in sugar beet roots, three procedures including microscopical, serological and molecular methods were compared on a sugar beet cultivar susceptible to causal agent and vector of rhizomania disease.At first, susceptible cultivar Regina was cultivated in infested soil in greenhouse condition. After 5 weeks, roots of plants were stained with fuchsin- acid- lactophenol solution. Quantitative ELISA test was optimized with specific antiserum against recombinant protein glutathione stransferase (GST) of P. betae. Running ELISA test, extracts of health and infected plants were prepared and used in DAS- ELISA test. Presence of P. betae in roots of plants was detected with amplification of rDNA of Plasmodiophoromycetes and P. betae species, simultaneously. Existence of cystosori of P. betae was observed in all of the plants planting in infested soil.Results of ELISA test were distinguished healthy plants from infected plants. Average of optical density at 405 nanometer for healthy plants, infected plants, protein PBS (negative control) and GST (positive control) was 0.126, 0.75, 0.11 and 2.45 respectively. Duplex PCR method was amplified two fragments of 454bp and 170bp in infected samples relating to rDNA region and specific region of the P. betae, respectively. Based on the results, it seems that, rapid detectionand identification of P. betae in sugar beet and other hosts of the vector could be done on the basis of PCR method. Evaluating of resistance germplasm of sugar beet to P. betae could be recognized using quantitative ELISA tests.