جستجوی مقالات مرتبط با کلیدواژه « molecular analysis » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

In order to investigate different lines of durum wheat for water stress tolerance, an experiment was carried out in the greenhouse in the form of a split plot design in a Randomized complete block design with three replications using 20 lines of durum wheat at two levels of water stress (40% and 70% of the amount of available water was completely drained in 0.4 and 0.7 levels of stress, respectively and re-irrigation was performed) and a control level (complete irrigation). Also, water-soluble and salt-soluble proteins were extracted and SDS-PAGE was done. Measured traits were photosynthetic pigments (chlorophyll a, chlorophyll b, chlorophyll a/b, total chlorophyll and carotenoids), soluble sugar and prolin content. The results of the analysis of variance showed that there was a significant difference between the studied lines at different levels of stress. The results of comparing the mean of the studied traits showed that in total, lines 16 and 5 have the highest value in most of the traits and lines 10 and 18 have the lowest value in most of the traits. Also, the results obtained from the cluster analysis indicated that due to the higher mean values compared to the other lines both in stress-free and stress conditions, lines 16 and 5 (Mra-1/4/Aus1/3/Scar/GdoVZ579//BitI, TRN//21563/AA/3/BD2080/4/BD2339/5/RASCON_37/TARRO_2//) have a higher performance stability in the different environmental conditions. Furthermore, the results of molecular analysis of the studied proteins showed that in water and salt soluble proteins based on matrix similarity and genetic distance of straw, the lowest genetic distance was between genotypes 18 and 19 (0.32) and the highest genetic distance was between genotypes 16 and 8 (0.98). Finally, it can be concluded that there is a significant genetic diversity between the studied cultivars and this diversity can be used in plant breeding.

Increasing the world population and subsequently increasing demand for food has led to the use of new biotechnology methods to produce food. Transgenic plants are one of the major biotechnological achievements in agriculture that have gained part of the world food market in recent years and the cultivation area of these plants is constantly increasing. Human needs to increase agricultural production and food security, the use of biotechnology has made it inevitable and on the other hand, the potential dangers of producing these products have raised concerns for human health and consumer rights. One of the concerns expressed by people is transferring unknown genes to plants from other countries and importing these products into the country without using GMO label, that these products may have an impact on human health and other living organisms. Therefore, first step is identifying and labeling transgenic products. In this study, 30 samples of domestic and foreign rice were randomly purchased from Shiraz market. DNA was extracted using the standard CTAB method and the polymerase chain reaction for SPS gene was performed to prove the presence of rice genome. The PCR for EPSPS gene, 35S promoter and NOS terminator were performed to identify transgenic rice. The results of PCR using specific primers showed that none of the rice samples purchased from Shiraz market were transgenic.

In 2017, leaf stunting and yellowing were observed on twigs and branches of stone fruit trees in production regions in Razavi and North Khorasan provinces of Iran. For diagnosis of disease and identification of the causal agent, symptomatic leaf samples were collected in these orchards and were carried to the laboratory. The disease agent was successfully transmitted by grafting from naturally symptomatic samples to peach (Prunus persica) seedlings. Total DNA was extracted from plant samples and indexed by nested PCR using phytoplasma generic primers, P1/P7 and R16F2n/R2. PCR products were sequenced and the nucleotide sequences were compared with those of other phytoplasmas in GenBank and analyzed by virtual restriction fragment length polymorphism (RFLP). The phytoplasmas associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces were identified as members of the 16SrIX group or Pigeon pea witches broom (Candidatus Phytoplasma phoenicium). This is the first report of leaf stunting and yellowing disease and characterization of associated phytoplasma in these regions.

Stability and gene expression are very important in the plant transformation. In this research the transgenic alfalfa plants containing cry3Agen (resistant insect pests) from Bacillus turingiensis var. tenebrionis were analyzed. In This study, transgenic alfalfa that were grown in the regeneration medium MS without hormones, were transferred to pots, and the presence of transgen was confirmed by PCR and Sothern blot hybridization. Expression of the Cry3A protein in transgenic plants was also confirmed by using DAS ELISA kit. The bioassey analysis of transgenic plants for alfalfa weevil larvae showed that percentage of mortality of larvae in control plants were 13-30%, while in transgenic plants it was observed between 33-90%, that was statistically significant (α<0.01). Average of percentage of leaf damage in control plants were 67-85% while amount of leaf damage in transgenic plants significantly reduced to 18-21%. Average live weight of larvae in the control plants was 0.02 mg but in transgenic was 0.001 mg.

Psoralen is one of the important secondary metabolites in Psoralea corylifolia. The important aspect of medicinal value of psoralen can be indicated to anticancer activity against leukemia, lung and breast cancers. In this research due to the pharmaceutical importance of psoralen, pathway of psoralen synthesis was studied. For the first time we isolate and characterized the gene coding psoralen key enzyme from the leaves of the Psoralea corylifolia by the different molecular techniques. Result of molecular analysis revealed that the length of this gene was 1237 bp. Also, the sequence analysis of nucleotides by using available bioinformatics data in NCBI found that psoralen synthase gene has 93% similarity with this gene in Ammi magus.