جستجوی مقالات مرتبط با کلیدواژه « native fowls » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the first step of the gluconeogenesis cycle. There are two isozymes forms of this enzyme, cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) and mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) which present in mitochondria and cytoplasm Current research has been conducted to identify the allelic polymorphism in the PEPCK-M gene in breeder hens of native fowls and commercial broiler and layer chickens. Blood samples were collected randomly from 150 birds of three strains and DNA was extracted using modified salting out method. A fragment of 401 bp in length was amplified from exon 9 of PEPCK M gene. For genotyping of each sample the PCR products were digested by AccI restriction enzyme. The frequency of AccI- and AccI+ allele was estimated at 0.73 and 0.27 in native fowls population, 0.6 and 0.4 in broiler and 0.85 and 0.15 in layer lines, respectively. Two genotypes of AccI -/- and AccI -/+ were observed with the frequency of 0.46 and 0.54 in native fowls, 0.20 and 0.80 in broiler line and 0.70 and 0.30 in commercial layer line, respectively. The comparison of allelic frequency showed no statistical differences between native fowls population with broiler and layer lines, but this results indicated significantly differences between broiler and layer lines (P<0.05).The comparison of genotypic frequency showed that there were significant differences between three populations (P<0.05). No any AccI +/+genotype was detected in the genotyped samples. The difference in genotypic distribution may be as a consequence of different selection strategies used in these populations.

In order to detect polymorphism in TGF-b3 loci and effect of this gene on phenotypic and breeding values of body weight traits, blood samples were collected randomly from 120 hens of Fars native fowls. DNA was extracted using standard kit and a DNA fragment of 294 bp from TGF-b3 loci was amplified. After PCR, the amplified fragment digested using BsrI enzyme and revealed two alleles T and t with the frequency of 0.29 and 0.71, respectively. The frequencies of TT, Tt and tt genotypes were achieved 0.15, 0.275 and 0.575 respectively. Values for Shanon Index, Effective number of alleles, observed heterozygosity and expected heterozygosity for TGFb3 were 0.60, 1.69, 0.28 and 0.41 respectively. Results showed that this population has a good genetic diversity and must try to conserve it to use these valuable gene pools. T allele had significant effect on body weight gain at 1 day and 8 weeks (P 0.05). Our results demonstrated that this population has a good genetic diversity, thus these valuable gene pools should be conserved to be used in the future if needed. TGF-b3 gene can also consider as a candidate gene in breeding of this animal.