جستجوی مقالات مرتبط با کلیدواژه "pgrs" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Improving plant regeneration skills in tissue culture studies is critical not only for the efficient genetic transformation of commercial crops but also for scientific reports. SNP (Sodium nitroprusside) as a Nitric oxide (NO) donor, plays an important role in the growth and development of plants. In this study, regeneration and plantlet development of Lycopersicon esculentum Mill. was improved using optimized concentrations of plant growth regulators supplemented with sodium nitroprusside. According to the results, among 12 different combinations of plant growth regulators, the MS medium complemented with 2 mg L-1 BAP and 0.2 mg L-1 IAA had a maximum percentage of regeneration (84%). The highest stem length (4.6 cm) and leave number (7) were achieved on MS medium supplemented with 0.5 mg L-1 BAP and 0.2 mg L-1 IAA. Adding of 10 μM sodium nitroprusside to the regeneration medium improved shoot regeneration efficiency (93%) and the number of shoots per explants (7.75). Furthermore, the maximum shoot growth mean, including stem length (11.8) and leaf number (11.2) were achieved on MS medium containing BAP (0.5 mg L-1), IAA (0.2 mg L-1), and 10 μM sodium nitroprusside. It was found that fewer adventitious roots and higher lateral roots were significantly developed in the medium containing IAA and SNP. Our findings indicated that adding SNP to the regeneration medium of L. esculentum Mill. improved shoot regeneration and plant development. This may overcome the problems in proliferation of the tomato plant.

The present study has been designed and executed to determine the best growth-regulating compound for callus induction as well as to specify the optimum dose of gamma irradiation in carnation cultivars (Tabasco, Nobless, Cameron, Tabor, Eskimo, and Mariposa). In this experiment, an MS culture medium was used to evaluate the various levels of growth regulator concentrations including NAA in four levels (0, 0.5, 1, and 2 mgl-1), 2,4-D in five levels (0, 0.5, 1, 2, and 3 mgl-1), and BA in two levels (0.5 and 1 mgl-1). Irradiating the callus of leaf explants was carried out three weeks after cultivation at 0, 15, 25, 35, 45, and 55-gray doses-to determine the optimum dose of gamma radiation. The analysis of data and illustration of graphs were carried out via Excel software and according to the obtained results, the radiation level that killed 50% of the calluses was selected as the optimum dose for further experiments. The results have indicated that all main effects and the interaction effects regarding the characteristics of callogenesis percentage and callus volume were significant at a probability level of 1%. Means were grouped using Duncan's multiple range test, revealing that the highest level of callus induction was in Eskimo cultivar with a 73% overall mean. Overall, the results indicate that 2 mgl-1 2,4-D, 0.5 mgl-1 BA is the best regulatory compound for callogenesis in carnation cultivars. Moreover, it was found that on average, the 25-gray dose leads to suitable results in the callus explants of all cultivars.

Callus induction and regeneration of fennel from embryo explants were stabilized in the presence of cefotaxime antibiotic and different plant growth regulators (PGRs). The experiments were conducted under a factorial experiment, based on a completely randomized design (CRD). Genotypes; Fasa, Meshkinshar and Hajiabad were applied under different concentration of cefotaxime (0 and 100 mg l-1), NAA (0 and 0.2 mg l-1), IAA (0 and 0.4 mg l-1) and BAP (0, 0.5 and 1mg l-1). Regeneration, proliferations and root induction were taken placed on studied media, after 35 days without sub-culturing. The highest rate of proliferation with 200 shoots per explant was observed on B5 medium, containing100 mg l-1 cefotaxime and 1.0 mg l-1 BAP.  Callus induction and proliferations were observed in all media containing 100 mg l-1 cefotaxime that can be related to auxin like activity of cefotaxime in fennel tissue culture.