جستجوی مقالات مرتبط با کلیدواژه « tylcv » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Tomato yellow leaf curl disease (TYLCD) is one of the most important diseases of tomato (Solanum lycopersicum L.) plants in tropical, subtropical regions and greenhouses in temperate regions of the world. To produce an antibody against the viral agent of the disease for use in a diagnostic kits, the TYLCV cloned viral movement protein gene was expressed in an expression vector (pET26). The resulting recombinant protein was then injected into a rabbit. The prepared antiserum was used in western blot and ELISA analyses in order to assess its efficiency in virus detection. A 13KD protein band related to viral movement protein was detected in infected plants by using Western blot analysis at 1:1500 antiserum dilutions, while in ELISA tests at 1:1000 dilutions of antiserum, denaturation of the protein could increase the differences between the absorbance values of the infected plants and non-infected plants. However, these differences were not statistically significant. The results showed that the prepared antiserum against Tomato yellow leaf curl virus- movement protein was suitable for western blot analyses, but to be used in ELISA test the antibodies need to be extraction and purification of antibodies.

Tomato yellow leaf curl virus (TYLCV) and a complex of ten virus species and their strains referred to as TYLCV-like viruses cause damage on tomato (Solanum lycopersicum L.) crops in tropical, subtropical and temperate regions of the world. In this study, full-length genome of three TYLCV-IL isolates originating from Datura stramonium L. in Bojnurd region, Iran were cloned, sequenced and subsequently entered the phylogenetic analysis with nine TYLCV-IL isolates from Iran and eight selected begomoviruses of tomato from Iran and other part of the world. The phylogenetic analysis suggested the geographical-dependent evolution of Iranian TYLCV-IL populations so that regardless of host species the monophyletic group of the northeastern and southern isolates was clustered separately into two subgroups. TYLCV-IL [IR: Sh40:07], GU076444 isolated in Shiraz region was not clustered with none of the Iranian TYLCV-IL isolates but rather was grouped with the type strain of TYLCV-IL from Israel in a separate group. Evolutionally, this isolate could be a “genetic bridge” between Mediterranean and Iranian TYLCV-IL isolates. This is the first report of Datura stramonium L. as the host species of TYLCV-IL in Iran and also the first-time occurrence of the viral stain in North Khorasan province, Bojnurd, Iran.

Tomato yellow leaf curl virus (TYLCV) is considered as one of the most important tomato pathogens in Iran. During the years 2007 to 2009, a total number of 284 symptomatic samples were collected from tomato fields and greenhouses from eight provinces of the country. The PCR test showed that 153 tomato samples from six provinces were infected by TYLCV. This is the first report of TYLCV in Rezvan (Hormozgan), Dashtestan (Bushehr),Dezful (Khuzestan), Pakdasht and Islamshahr (Tehran) regions. Based on geographical region and symptoms, nine isolates from six provinces were selected for further studies.Comparison of an amplified DNA fragment of 670 bp, including a part of coat protein and movement protein genes of the virus, with those of previously reported isolates from Iran and closely related isolates available in GenBank, suggested that Iranian isolates examined in this study were grouped into two major groups. Isolates collected from Hormozgan, Khuzestan and Kerman provinces were grouped with other Iranian isolates including TYLCV-Ir2 and TYLCV-Kahnooj and an isolate from Oman. Whereas, isolates collected from Bushehr, Fars, and Tehran were placed close to the Iranian isolate of TYLCV-Abadeh and isolates from occupied Palestine and Egypt. Based on our study, TYLCV has a high genetic diversity in Iran.