جستجوی مقالات مرتبط با کلیدواژه "فلس" در نشریات گروه "زراعت"

Background and Objectives Lilium ledebourii is one of the wild species of Lilium genus, growing in northern Iran. This species has high potential for export. Plant tissue culture techniques are widely used in plant propagation, and using these methods can effectively provide micro-propagation of this plant in the large scale. High percentage of regeneration is necessary for plant protection, used in the breeding programs.
Materials and Methods The experiment was factorial based on completely randomized design with four replications and was carried out in tissue culture Lab of Mohaghegh Ardabili University in 2015. For this purpose, segments of explant that were treated with ultrasound were cultured on MS medium supplemented with different concentrations of NAA and BA alone and/or in combination with each other. This experiment conducted Concentrations of NAA at four level Zero, 0.01, 0.1 and 1 mgl-1 and Concentrations of BAP at four levels Zero, 0.01, 0.1 and 1 mgl-1 and Ultrasound at four levels zero, 5, 10, 20 and 30 seconds. In order to remove possible contamination from the mediums, all media were autoclaved for 30 minutes at 121 °C. At the end of the experiment, the number of bulblet produced, root length, fresh weight of bulblet and etc were recorded. The cultures were kept at 20°C or 25°C under 16 h photoperiod or in darkness.
Results The results showed the highest number of bulblets produced in 0, 0.01, 0.1 mg L-1 NAA and 5secend ultrasound, 0.1 mg L-1 NAA and 0, 5, 20 and 30 second ultrasound compared with control. The highest frequency of bulblet regeneration was produced in MS medium supplemented with 0, 0.01, 0.1 mg L-1 NAA and 5 second ultrasound. On the other hand, 0.1 mg L-1 NAA and 5 second ultrasound increased number and weight of bulblets. Different concentrations of NAA had also significant effects.
Discussion On the other hand, ultrasound increased the number and weight of bulblets. Mechanical stress and micro streaming by acoustic cavitation might be considered as the most possible cause of the various physiological effects of ultrasound on cells. Low-energy ultrasound can modify cellular metabolisms or facilitate the uptake of nutrient, and make them easily through the cellular walls and membranes. Finally, it seems that ultrasound in combination with growth regulators have the potential to produce the highest average number of bulblets in the scale explant, using plant growth regulators in combination with an abiotic stress in in vitro.

Fritillaria imperialis is a mono-cotyledon plant from the Liliaceae family that grows wild in some parts of Iran. Fritillaria is a medicinal plant، in addition to being a beautiful flower. Due to the uncontrolled harvesting of flower and bulbs، Fritillaria survival is threatened in some areas in Iran. Fritillaria can be propagated by seed. In favorite condition. Its propagation by seed and production of flower-beating bulbs takes about 6 years. In addition، plants grown from seed would not be like their parents due mainly to occurrence of cross-polination. Mass propagation of Fritillaria through conventional methods such as bulb culture is not efficient. Thus، its in vitro propagation as a criteria for preserving this valuable plant in its natural habitats was studied. We investigated direct embryogenesis of sterilized bulbs scale segments in MS medium at the presence of different plant growth regulators including NAA، IAA، TDZ، Kinetin and BA and the combination of kinetin and NAA in different concentrations. The highest number of direct somatic bulblets (8. 33 bulblets)، the biggest bulblets (26 mm) and the greatest number of roots (8 roots) were formed in 2 mg/L NAA. The least direct bulblet somatic generation was observed in TDZ treatments، bulblets formed tended to produce callus instead of growing. In the medium containing BA hormone no bulblet was produced. In conclusion، the results of this study revealed that propagation of Fritillaria imperialis is possible through in vitro culture by using 2 mg/L NAA.

This study was conducted to investigate the effects of in vitro and regeneration of lily (Lilium longiflorum) bulb scale by different plant growth regulators in B5 medium in a completely randomized design with factorial arrangement and 4 replications. Treatments consisted of indole-3-acetic acid (IAA) and 2,4-D in B5 medium in four concentrations (0, 0.5, 1, 2 mg/lit) and these were combined to medium. Evaluation of results indicated that with increasing of IAA concentration, number of bulblets increased significantly but between using of various IAA concentrations and rooting parameters and callus formation, no significant difference was observed. With increasing of 2,4-D concentration to 2 ppm, callus fresh weight was enhanced, whereas with increasing of 2,4-D concentration, organogenesis suppressed completely. According to the results, about 100-150 plantlet were achieved per adult bulb of lily.