جستجوی مقالات مرتبط با کلیدواژه "کلون ها" در نشریات گروه "زراعت"

In this study, 33 pototao genotypes including 31 advanced potato clones obtained from breeding programs with two commercial cultivars Agria (suitable for French-fries) and Caesar (suitable for fresh eating) as controls were evaluated for agronomic traits, yield stability and quality properties using randomized complete block design with three replications in 2015-2018 in five experimental filed stioans; Karaj, Ardabil, Mashhad, Isfahan and Hamadan in Iran. Clones were propagated at Ardebil agricultural and natural resources research station in 2015 to supply required potato tubers for planting in five locations. During growth and after harvest, tuber plant-1 and tuber weight plant-1, plant height, main stem plant-1, tuber shape, depth of the eyes, tuber skin and flesh colour, duration of growth, deformed tubers, tuber growth cracks, tuber hollow heart, inner ring rot of tuber, change of raw tuber flesh colour and tuber dry matter percentage were measured in Ardabil and Hamadan, and total and marketable tuber yield in five locations; Ardabil, Hamadan, Karaj, Mashhad and Isfahan. Combined analysis of variance showed that genotype, year, location, location × year, year × genotype, location × genotype and location × year × genotype significantly affected total and marketable tuber yield. Clones no. 1 (1675), 5 (1375), 8 (5675) and 26 (1027) had the highest tuber yield, respectively. Based on the results of GGE bi-plot model, high yielding clones; no. 1 (1675) and no. 8 (5675) had high yield stability and medium-early maturity, and clones; no. 5 (1375) and no. 26 (1027) had average yield stability and medium-late maturity. Clones no. 5 (1375), no. 8 (5675) and no. 26 (1027) had high tuber dry matter percentage, light yellow flesh and skin colour, without deformed tubers, without tuber hollow heart, without tuber growth cracks, good coverage, low change in flesh colour of raw tuber, low and shallow eye depth. Finally, clones 1675, 1375, 5675 and 1027 were selected as promising clones with high tuber yield and yield stability. In 2021, clone 1675 released as new cultivar with the name of Takta and clone 1027 released with the name of Rona, and are commercially available to potaoto growers in Iran.

In interspecific and intraspecific crosses, pre-zygotic barriers like pollen-pistil incompatibility may exist, and in the case that a parent is selected as male or female, this incompatibility may disappear. In this investigation seven high yielding commercial cultivars of potato (Agria, Savalan, Picasso, Caesar, Daifla, Luca and Satina) were reciprocally crossed with eleven promising potato clones in Ardebil Agricultural and Natural Resources Research Station during the summer of 2014 to obtain cheep TPS hybrids. The best cultivars and clones were appointed when they were as male and female. Results of two pollen fertility tests showed that cultivars Agria and Luca and clones AS10 and AS72 were sterile and did not produce fruit when participated as mate parents in crosses. Generally, when commercial cultivars were as female, the number of fruit and seed, seed weight, cross efficiency, strength and uniformity of germination and seedling growth were better.When commercial cultivars were considered as female parent and clones as male parent, Savalan, Caesar and Luca cultivars showed better traits than other cultivars. When clones were mate and commercial cultivars female, HS Clone and UT42 had higher percentages of fruit and seed set, crossing efficiency, percentage of seed germination and 100-seed weight. In crossing, when the commercial cultivars were male and clones were female parents, clones AS72 and AS10 had better traits than the other Clones.

In the present study, resistance of 29 potato clones to fusarium dry rot disease was compared with some commercial cultivars and a susceptible check cultivar Lady Roseta. Tubers of the potato clones and cultivars were artificially inocutate with aggressive isolates of Fusarium sambucinum and F. solani and maintained in a growth chamber at 16°C and 95% relative humidity for four weeks. The experiment was in factorial with two factors of Fusarium species and potato clones, using three replications. Disease index, infected area of tubers and sprouting rate were recorded for each treatment after four weeks. Based on the results of experiment, potato clones 397009, 39709713, 39708-10, 397097-2, 397007-16, 397074-3 and cultivar Aria having the lowest disease index (ranging from 168.52 to 228.25) and lowest tuber infected area, were considered as resistant, while clones 396124-97 × 396151-15 and 396151-29 with highest disease index and tuber infected area were susceptible. Fusarium species had an invert effect on tuber sprouting so that susceptible potato clones and cultivars had the least tuber sprouting rate.

Polyhydroxybutyrate (PHB) is a polyhydroxy alkanote (PHA) which produces in ralstonia eutropha by three enzymes, including Beta-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase and PHB synthase. These three enzymes are encoded by phbA, phbB and phbC respectively. There is considerable interest in Polyhidroxybutirate, since it can be used as biodegradable plastic, which under optimal conditions are degraded completely to CO2 and H2O. In this research phb operon was isolated from ralstonia genome using polymerase chain reaction. Specific primers were designed for PHB isolation. The PCR product was purified and cloned. Accuracy of isolation and cloning of three PHB genes were verified by nested PCR and digestion reaction. It is expected that cloning of PHB genes in expression and transformation vectors can be caused to cost effective production of Polyhydroxybutyrate as a bio-degradable plastic in plants.