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تکرار جستجوی کلیدواژه «b» در نشریات گروه «کشاورزی»
  • راهله رهباریان*، الهام عزیزی، آسیه بهداد، آتنا میربلوک

    به منظور بررسی اثرات سرب و جاسمونات بر شاخص های فتوسنتزی بادرشبو، آزمایشی در شرایط گلخانه با پنج سطح سرب (صفر، 100، 200، 300 و 400 میلی گرم بر کیلوگرم خاک) و چهار سطح جاسمونات (صفر، 50، 100 و 150 میلی مول بر لیتر) در خاک غنی شده با ورمی کمپوست و بدون آن انجام شد. آزمایش به صورت فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار انجام شد و میزان اسیمیلاسیون دی اکسید کربن، تعرق و کارایی مصرف آب، هدایت روزنه ای، نرخ انتقال الکترون فتوسنتزی (ETR)، عملکرد کوانتومی فتوسنتز (Y) و عملکرد فتوسیستم ΙΙ (Fv/Fm) گیاهان در دو مرحله رشد رویشی و زایشی موردسنجش قرار گرفت. نتایج حاصل نشان داد که افزایش غلظت سرب اثر کاهشی بر میزان اسیمیلاسیون، کارایی مصرف آب، عملکرد فتوسیستم ΙΙ و هدایت روزنه ای داشت. جاسمونات میزان اسیمیلاسیون، کارایی مصرف آب، هدایت روزنه ای و عملکرد فتوسیستم ΙΙ را به طور معنی داری افزایش داد. به طوری که گیاهان در تیمار 400 میلی گرم بر کیلوگرم خاک سرب و صفر میلی مول بر لیتر جاسمونات، کم ترین میزان اسیمیلاسیون، کارایی مصرف آب و عملکرد فتوسیستم ΙΙ و در تیمار با غلظت صفر سرب و 150 میلی مول بر لیتر جاسمونات بیش ترین میزان اسیمیلاسیون، کارایی مصرف آب و عملکرد فتوسیستم ΙΙ را به خود اختصاص دادند (P≤0.05). میزان تعرق نیز با افزایش سرب افزایش یافت به طوری که گیاهان با سرب 400 بیش ترین تعرق را در هر دو مرحله رشد نشان دادند (P≤0.05). جاسمونات روند تغییرات تعرق ناشی از سرب را کاهشی نمود. اعمال کود سبب افزایش معنی دار اسیمیلاسیون، نرخ انتقال الکترون فتوسنتزی و کارایی مصرف آب و کاهش معنی دار تعرق در شرایط تنش سرب گردید. بر این اساس می توان گفت که سرب احتمالا با تاثیر بر مسیر نوری فتوسنتزی و اختلال در مسیر انتقال الکترون فتوسنتزی سبب کاهش اسیمیلاسیون دی اکسید کربن و کارایی مصرف آب گشته است و همچنین تیمار جاسمونات و ورمی کمپوست اثرات تخریبی سرب را جبران نموده است.

    کلید واژگان: اسیمیلاسیون دی اکسید کربن, بادرشبو, فلز سنگین, کود, Fv, Fm}
    Raheleh Rahbarian *, Elham Azizi, Asieh Behdad, Atena Mirblook
    Introduction

    Dracocephalum moldavica L. is a herbaceous, annual plant from the Lamiaceae family that is native to Central Asia and domesticated in Central and Eastern Europe. Essential oil of this plant has antimicrobial and bacterial properties and has many uses in the pharmaceutical, cosmetic, food and perfumery industries. Lead stress in plants causes disturbances in mitosis, leaf chlorosis, decreasing of the vegetative and productive growth stages and reduces photosynthesis and enzyme activities. One of the effects of lead toxicity is due to the similarity of the structure of calcium ions and lead, and for this reason, lead ions disrupt many mechanisms related to calcium ions and prevent the activity of key enzymes.Photosynthesis is one of the most sensitive metabolic processes to lead toxicity, and several studies have reported the inhibition of photosynthesis under lead stress in various plants. Lead prevents the absorption of elements such as magnesium and iron. These elements play a role in the structure of chlorophyll and the oxygen-releasing complex in photosystem II. Heavy metals such as lead inhibit chlorophyll biosynthesis by inhibiting the enzymes gamma-aminolevalonic acid dehydrogenase and protochlorophyll reductase. Also the availability of different nutrients in the soil changes significantly under the influence of environmental stress so that using of vermicompost can be useful in stress condition as well as Jasmonate. Jasmonate is the final oxidation product of unsaturated fatty acids such as linolenic acid, that is effective in increasing the activity of plant defense systems under environmental stress conditions such as lead stress. In order to study the effect of pb (0, 100, 200, 300, 400 mg kg-1 soil) and jasmonate (0, 50, 100, 150 mmol l-1) on Dracocephalum moldavica L. under controlled conditions in soil enriched with vermicompost and without vermicompost an experiment designed and it was done under greenhouse conditions.

    Materials and methods

    This test was done in a random factorial design with 4 repeats and indices including CO2 assimilation rate, transpiration rate, stomatal conductance, water use efficiency(WUE), PSɪɪ photochemical efficiency (Fv/Fm), photosynthesis quantum performance, electron transfer rate (ETR), were measured in vegetative and reproductive growth stages.

    Results and discussion

    In the conducted study, it was found that CO2 assimilation rate, water use efficienty, stomatal conductance and Fv/Fm were significantly decreased as lead concentration was increased. Also jasmonate treatment significantly increased CO2 assimilation rate, water use efficienty, stomatal conductance and Fv/Fm in lead stress condition. So that plant treated with 400 mg kg-1 soil pb and 0 mmol l-1 jasmonate showed the lowest CO2 assimilation rate, water use efficienty, stomatal conductance and Fv/Fm while plant treated with 0 mg kg-1 soil pb and 150 mmol l-1 jasmonate showed the highest CO2 assimilation rate, water use efficienty, stomatal conductance and Fv/Fm. transpiration rate was significantly increased as well as increasing pb concentration so that plant treated with 400 mg kg-1 soil pb showed the highest transpiration rate. Also jasmonate treatment significantly decreased transpiration rate in lead stress condition. Vermicompost increased CO2 assimilation rate, water use efficienty, electron transfer rate (ETR) and significantly decreased transpiration rate in lead stress condition.Jasmonate reduces the destructive effects caused by stress on photosynthetic indicators such as the amount of chlorophyll and carotenoids and also increases the performance of photosystem II and consequently increases plant photosynthesis under stress conditions. It has been reported that methyl jasmonate can maintain the concentration of chlorophyll in the reaction center, thereby improving the speed of electron transfer and increasing the efficiency of photosystem II. In addition, jasmonate can prevent the severe reduction of stomatal conductance under stress conditions and increase the quantum efficiency of photosynthesis.In the response of plants to stress, jasmonates act as genes encoding inhibitory proteins such as theonine, hydroxyproline and proline, and in general, by activating defense mechanisms, they help the plant in reducing the absorption and accumulation of heavy metals. Organic fertilizers can also improve plant performance under environmental stress conditions. Vermicompost fertilizer increases porosity, increases absorption and retention of nutrients, improves ventilation, drainage and microbial activity in the soil.

    Conclusion

    Having nutritious mineral elements and plant growth hormones can improve plant growth in the presence of environmental pollutants such as heavy metals by influencing the physiological characteristics. It can be said based on the results obtained lead stress decreased photosynthesis index through effect on electron transport chain and photosynthetic pigments while jasmonate treatment and Soil enriched with vermicompost can reduce the destructive effects of lead stress. So that using of jasmonate and vermicompost in lead stress condition Recommended.

    Keywords: CO2 Assimilation Rate, Dracocephalum Moldavica L, Fertilizer, Fv, Fm, Heavy Metal Stress}
  • فاطمه آقایی*، رئوف سیدشریفی، سلیم فرزانه

    به منظور بررسی اثرات برخی تعدیل کننده های تنش (باکتری های محرک رشد و نانواکسید آهن و سیلیکون) بر عملکرد و برخی صفات فیزیولوژیکی و بیوشیمیایی تریتیکاله در شرایط محدودیت آبی، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوک کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 1400 اجرا شد. فاکتورهای مورد بررسی شامل آبیاری در سه سطح (آبیاری کامل به عنوان شاهد، قطع آبیاری در 50 درصد مراحل چکمه ای شدن (آبستنی) و سنبله دهی به ترتیب به عنوان محدودیت شدید و ملایم آبی به ترتیب کد 43 و 55 مقیاس BBCH)، کاربرد کودهای زیستی در چهار سطح (عدم کاربرد به عنوان شاهد، کاربرد آزوسپریلیوم، سودوموناس، کاربرد توام آزوسپریلیوم و سودوموناس) و محلول پاشی نانوذرات در چهار سطح (محلول پاشی با آب به عنوان شاهد، محلول پاشی یک گرم در لیتر نانو اکسید آهن، محلول پاشی50 میلی گرم در لیتر نانوسیلیکون، محلول پاشی توام نانواکسید آهن و نانوسیلیکون) می شدند. نتایج نشان داد کاربرد باکتری های محرک رشد و نانو اکسید آهن وسیلیکون در شرایط آبیاری کامل محتوای کلروفیل کل (48.16%)، عملکرد کوانتومی (36.05%)، محتوای نسبی آب (35.83%) و عملکرد دانه (43.28%) را نسبت به عدم کاربرد باکتری های محرک رشد و نانوذرات تحت شرایط قطع آبیاری در مرحله آبستنی افزایش داد. همچنین کاربرد باکتری های محرک رشد و نانوذرات تحت شرایط قطع آبیاری در مرحله آبستنی فعالیت آنزیم های کاتالاز، پراکسیداز و پلی فنل اکسیداز را (به ترتیب، 47.06، 55.69 و 36.53%) و محتوای پرولین، قندهای محلول (به ترتیب 45.41 و 46.93%) نسبت به عدم کاربرد باکتری های محرک و نانوذرات تحت شرایط آبیاری کامل افزایش داد. بر اساس نتایج این بررسی به نظرمی رسد باکتری های محرک رشد و نانوذرات می تواند بواسطه بهبود صفات فیزیولوژیکی و بیوشیمیایی، عملکرد دانه را در شرایط محدودیت شدید آبی افزایش دهد.

    کلید واژگان: آنزیم های آنتی کسیدانی, اسمولیت های سازگار, سودوموناس, محتوای نسبی آب, نانواکسید آهن}
    Fatemeh Aghaei *, Raouf Seyed Sharifi, Salim Farzaneh
    Introduction

    Water limitation is one of the most important abiotic factors that can limit plant growth and yield due to production of reactive oxygen species (ROS) like H2O2 and the reduction of chlorophyll content. To protect against oxidative stress, plant cells produce both antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), and non-enzymatic antioxidants such as low weight molecules like proline, sugars and ascorbate. Also water limitation disturbs the mineral-nutrient relations in plants through their effects on nutrient availability and numerous of physiological and biochemical destruction in the vegetative and reproductive periods of plant development. Several strategies have been suggested in order to improve yield under abiotic and biotic stresses in plants, among them application of Plant Growth Promoting Rhizobacteria (PGPR) and nano particles such as nano iron-silicon oxide play a key role in yield improvement. A better understanding of physiological responses under water limitation may help in programs which the objective is to improve the drouht resistance of crop. During the course of these stresses, active solute accumulation of compatible solutes such as proline and the activities CAT, POD and PPO enzymes are claimed to be an effective stress tolerance mechanism. Therefore, the aim of this study was to evaluate the effects of bio-fertilizers and nano iron oxide and nano oxide on some physiological and biochemical (i.e., antioxidant enzyme activity, chlorophyll, protein, soluble sugars and proline) responses of triticale under water limitation conditions.

    Materials and methods

    An experiment was conducted as factorial based on randomized complete block design with three replicates at the research farm of faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2021. The experimental factors were included of irrigation in three levels (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe and moderate water limitation respectively (BBCH 43 and 55 respectively), application of bio fertilizers in four levels (no application as control, application of Azospirilum, Pseudomonas, both application Azospirilum and Pseudomonas) and nanoparticles foliar application at four levels (foliar application with water as control, nano iron oxide foliar application (1 g.L-1), nano silicon oxide (50 mg.L-1), both application nano iron-silicon oxide). Psedomunas and Azospirilum were isolated from the rhizospheres of wheat by Research Institute of Soil and Water, Tehran, Iran. For inoculation seeds were coated with gum Arabic as an adhesive and rolled into the suspension of bacteria until uniformly coated. The strains and cell densities of microorganisms used as PGPR in this experiment were 1×108 colony forming units (CFU). In each plot there were 5 rows with 2 m long. In each experimental plot, two marginal rows and 0.5 m from beginning and ending of planting lines were removed data were measured from the middle lines. The used nano silicon-iron oxide had the average particle size less than 30 nm and special surface of particles was more than 30 m2.g-1. They were product of Nanomaterial US Research which was provided by Pishgaman Nanomaterials Company of Iran. Nano iron oxide and nano silicon powder added to deionized water and was placed on ultra sonic equipment (100 W and 40 kHz) on a shaker for better solution. Foliar application of nano silicon oxide and putrecine were done in two stages of period growth BBCH 21 and 30.

    Results and discussion

    The results showed that total chlorophyll content (48.16%), quantum yield (36.05%), relative water content (35.83%) and grain yield (43.28%) increased in dual application of bio fertilizers and nano particles foliar application under full irrigation conditions compared to no application of bio fertilizers and nano particles under irrigation cut off at booting stage. But under such conditions, electrical conductivity, hydrogen peroxide and malondialdehyde content decreased 35.67, 53.16 and 56.32% respectively compared to no application of PGPR and nanoparticles under irrigation withholding in booting stage. Also, the application of PGPR and nanoparticles under irrigation cut off in booting stage increased the activity of catalase, peroxidase and polyphenol oxidase enzymes (47.06, 55.69 and 36.53% respectively), proline and soluble sugars content (45.41 and 46.93% respectively) compared to no application of PGPR and nanoparticles under full irrigation conditions.

    Conclusion

    Based on the results of this study, the application of plant growth promoting rhizobacteria and nonoparticle can increase grain yield of triticale under water limitation conditions due to improving biochemical and physiological traits.

    Keywords: Antioxidant Enzymes, Compatible Osmolytes, Nano Iron Oxide, Pseudomonas, Relative Water Content}
  • زینب تمسکی*، بابک عندلیبی، سجاد نصیری

    کمبود آب به مشکلی جهانی تبدیل شده و باعث مشکلات بسیاری در بخش کشاورزی و تامین غذا برای جمعیت رو به رشد جهان شده است به منظور بررسی اثر رژیم های مختلف آبیاری و محلول پاشی هورمون متیل جاسمونات بر خصوصیات رشدی، فیزیولوژیکی و بیوشیمیایی ارقام جو، آزمایشی به صورت طرح اسپلیت اسپلیت پلات در قالب طرح بلوک های کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه زنجان در سال زراعی 1399-1400 انجام شد. در این آزمایش، رژیم های آبیاری به عنوان اثر اصلی شامل آبیاری کامل (شاهد)، قطع آبیاری در مرحله گلدهی، قطع آبیاری در مرحله پر شدن دانه و دیم کامل، ارقام به عنوان اثر فرعی شامل رقم بهمن، سهند، جلگه، آبیدر و انصار و محلول پاشی متیل جاسمونات شامل بدون محلول پاشی (شاهد) و محلول پاشی 50 میکرومول بر لیتر متیل جاسمونات موردبررسی قرار گرفتند. نتایج مقایسات میانگین اثر متقابل سه گانه بین آبیاری و رقم و متیل جاسمونات در صفات کلروفیل a و کلروفیل کل ارقام جو قرارگرفته در معرض دیم در این صفات کاهش و در صفت پرولین در شرایط قطع آبیاری در مرحله گلدهی و پر شدن دانه و دیم افزایش مقدار پرولین را در مقایسه با آبیاری نرمال و نیز در شرایط دیم کاهش ارتفاع را در مقایسه با آبیاری نرمال نشان دادند. همچنین نتایج مقایسه میانگین اثر متقابل رقم و متیل جاسمونات در صفت عملکرد دانه نشان دهنده این است که در ارقام بهمن، سهند، جلگه و آبیدر محلول پاشی شده با متیل جاسمونات عملکرد دانه را به ترتیب 17.7، 24.6، 8.3 و 22.3 درصد در مقایسه با تیمار بدون متیل جاسمونات به طور معنی داری افزایش داد ولی در رقم انصار یک افزایش غیر معنی دار (5.4 درصد) در عملکرد دانه حاصل شد. بیشترین عملکرد دانه (4762 کیلوگرم در هکتار) زمانی مشاهده شد که رقم جلگه تحت تیمار آبیاری کامل با 50 میکرومول بر لیتر متیل جاسمونات محلول پاشی شد و کمترین عملکرد دانه (432 کیلوگرم در هکتار) در رقم بهمن در شرایط دیم و بدون محلول پاشی ایجاد شد. به نظر می رسد کاربرد متیل جاسمونات و آبیاری کامل به عنوان یک ابزار مناسب برای افزایش عملکرد دانه استفاده شود.

    کلید واژگان: ارتفاع, پرولین, تنش خشکی, رنگدانه های فتوسنتزی, مالون دی آلدهید}
    Zeynab Tamasoki *, Babak Andalibi, Sajjad Nasiri
    Introduction

    Water shortage has become a global problem and has caused many problems in agriculture and food supply for the growing world. Barley (Hordeum vulgare L.) as the fourth mostly-cultivated cereal, is one of the most strategic crop plants which is produced almost all over the world as a source of and important staple food and animal feed (Thabet et al., 2020). Food uncertainty is a comprehensive obstacle becomes more serious hazard all over the world in particular in developing countries for the sake of overpopulation and dwindling accessibility of croplands, water and other resources related to agricultural scopes. Water scarcity, results in plenty of disturbances in plant functions like cell division and elongation, water and nutrients relations, photosynthesis, enzymes activity, stomata movement, assimilate partitioning, respiration, oxidative damage, growth, and productivity, as several types of researches show that water shortage in the soil cause many disorders in plant tissues, which in turn leads to a punctual diminish in the photosynthesis rate (Todorova et al., 2022). In such a trouble circumstances, most of the plants are not capable to absorb abundant water, which is required for optimized growth (Danish et al., 2020).

    Materials and methods

    To investigate the impact of different irrigation regimes as well as foliar application of methyl jasmonate on growh, physiological and biochemical characteristics of barley varieties, an experimental research using factorial split plot design in 3 replications was carried out in experimental farm of the faculty of agriculture at the University of Zanjan in 2021-2022 cultivation season. In this experiment, irrigation regimes as the main factor, including complete irrigation as the control, withholding water in flowering stage, withholding water in grain filling period and complete dry farming, varieties as the secondary factor including Bahman, Sahand, Jolgeh, Abidar as well as Ansar, and foliar application of methyl jasmonate including without spraying (control) and spraying 50 μmol of methyl jasmonate were investigated. Results showed that the effect of irrigation regime had significant impact on almost all of the characteristics except chlorophyll a/b.

    Results and discussion

    Varieties showed considerable difference from the aspect of height, concentration of soluble sugar and grain yield. Effect of Methyl jasmonate on the relative water content (RWC), photosynthetic pigments, soluble sugar content, proline content, Malondialdehyde (MDA) and grain yield was significant. The highest grain yield (4762 kg ha-1) was detected when variety of Jolgeh was irrigated normally and was sprayed by 50 μmol of Methyl jasmonate and the lowest grain yield (432 kg ha-1) was seen when variety of Bahman was dry-farmed without foliar application of methyl jasmonate.

    Conclusion

    The current study illustrated that barley can be grown in drought stress conditions if the right management is set on its cultivation. Methyl jasmonate showed a significant impact on the growth characteristics, biochemical and physiological attributes of the barley, despite the fact that drought had substantial adverse effects on the studied parameters of barley. However, drought resulted in different properties in barley varieties.

    Keywords: Barley, Drought Stress, Height, Malondialdehyde, Photosynthetic Pigment, Proline}
  • شکیبا شاهمرادی*، آزیتا نخعی، سید علی طباطبایی

    به منظور ارزیابی و شناسایی ژنوتیپ های متحمل به شوری، تعداد 50 ژنوتیپ منتخب از آزمایشات شوری انجام شده در سال های گذشته، به منظور ارزیابی تکمیلی در شرایط تنش شوری، انتخاب شدند. ژنوتیپ ها در طی دو سال زراعی در قالب طرح لاتیس مستطیل با سه تکرار در ایستگاه مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان جنوبی، شهرستان بیرجند، مورد ارزیابی قرار گرفتند .آزمایش در شرایط نرمال و تنش شوری به طور جداگانه اجرا شد. به منظور مقایسه پتانسیل عملکرد این ژنوتیپ ها با ارقام اصلاح شده، شش رقم جو زراعی از جمله دو شاهد متحمل به شوری شامل ارقام جو مهر و خاتم نیز در آزمایشات گنجانده شد، لذا آزمایش در قالب طرح لاتیس مستطیل 8×7 اجرا شد (50 ژنوتیپ و شش رقم شاهد). صفات فنولوژیکی، میزان عملکرد دانه و وزن هزار دانه در ژنوتیپ ها اندازه گیری شد .به منظور گزینش ژنوتیپ های متحمل به شوری و با عملکرد بالا در هر دو محیط تنش و محیط بدون تنش، نمودار بای پلات براساس عملکرد در شرایط نرمال و شرایط تنش و رسم گردید و همچنین تجزیه و تحلیل روابط میان صفات و تحمل به تنش شوری در ژنوتیپ های برتر با استفاده از نرم افزارهای Stat Graphics و SPSS انجام شد. تجزیه مرکب صفات نشان داد، اثر متقابل سال و تنش شوری و ژنوتیپ بر صفات معنی-دار بود. در مجموع این نتایج نشان داد که تغییرات اقلیمی در سال های مختلف تاثیر زیادی بر واکنش ژنوتیپ ها نسبت به تنش شوری دارد. ژنوتیپ 32 (TN4104)، در هر دو سال در بین ژنوتیپ های برتر بود.

    کلید واژگان: اقلیم, تنش, تحمل, تنوع}
    Shakiba Shahmoradi *, Azita Nakhei, Seyed Ali Tabatabaie
    Introduction

    The combination of the effects of drought stress and soil salinity causes a severe limitation in the production of agricultural crops. Irrigation with saline water has caused the expansion of more saline lands. Salt tolerant genotypes are very important in the development of agricultural systems suitable for saline lands. Soil salinity through soil amendment and or the cultivation of tolerant crops can be adjusted. Of course, soil amendment is a costly process and the cultivation of tolerant species and varieties is the most practical solution in conditions where soil salinity is low. It is clear that the genotypes show a significant difference in response to salinity stress. One of the ways to deal with salinity is to select and find salinity-tolerant cultivars through the use of breeding methods. It is also possible to select and modify salinity-tolerant species in some fodder plants of temperate regions. This research was modified, with the aim of evaluation of selected barley genotypes from past years' experiments, under salinity stress conditions and identification and introduction of tolerant genotypes and direct and indirect use of them in breeding programs.

    Materials and methods

    In this experiment, the genotypes were evaluated during two crop years in the form of a rectangular lattice design with three replications at the research station of South Khorasan Agriculture and Natural Resources Research Center, Birjand. The experiment was conducted under normal conditions and salt stress separately. In order to compare these genotypes with modified cultivars, six cultivated barley cultivars, including two salinity-tolerant controls, including Mehr and Khatam cultivars and the semi-sensitive Yusuf control, were also included in the experiments (Table 1). In addition to evaluating the phenological traits of days to flowering and days to maturity, the traits of plant height, seed yield, and 1,000-seed weight were recorded. Stress indices including stress tolerance index (STI) were calculated based on grain yield in barley genotypes.

    Results and discussion

    The combined analysis of phenological, morphological and agronomic traits in the evaluated genotypes in two cropping years and two normal conditions and salinity stress showed that the interaction effect of year, salinity stress and genotype on the traits is significant. This showed that the reaction of genotypes was different in different years and different salinity conditions, so the results in different years were analyzed separately. In the salt research station in the first year of the experiment, genotypes number 31 (TN4006), 30 (TN3947), 50 (TN5008) and 28 (TN3646) along with Yusuf, Nusrat and Gohran cultivars, in terms of agronomic traits and stress indices were superior. While in the second year, the top genotypes were genotypes No. 44 (TN4904), 25 (TN3477), 23 (TN3470) and 32 (TN4104) along with Nimroz and Mehr cultivars.

    Conclusion

    The information related to the trend of temperature changes and rainfall in different months in Birjand showed that the amount of rainfall was significantly higher in the first year of the experiment. In addition to reducing the salinity of the soil by adjusting the temperature, it reduces the amount of evaporation and transpiration and the intensity of the salinity stress. Therefore, it seems that the level of salinity stress was milder in the first year and more intense in the second year, and this was also observed in the stress intensity index. Based on this, the difference in the results in the two years of the experiment can be justified, in other words, in the first year, tolerant genotypes were introduced in mild stress and in the second year, tolerant genotypes were introduced in severe stress. Overall, these results showed that climate changes in different years have a great impact on the response of genotypes to salinity stress.

    Keywords: Climate, Diversity, Stress, Tolerance}
  • رامین لطفی*

    گیاهان در محیط تحت تاثیر تنشهای مختلف زیستی و غیرزیستی قرار دارند که این تنش ها بسته به مدت، شدت و مرحله رشدی گیاه می توانند فرایند فتوسنتز را کاهش و رشد و نمو و عملکرد آن ها را تحت تاثیر قرار دهند. مطالعه فتوسنتز با روش هایی همچون آنالیز تبادلات گازی شامل دی اکسیدکربن، بخار آب و اکسیژن زمان بر بوده و اطلاعات کاملی از تمام ساختار دستگاه فتوسنتزی در اختیار قرار نمی دهند. بااین وجود، اندازه گیری و کاربرد تکنیک فلورسانس کلروفیل روشی بسیار ساده، غیرتخریبی و سریع برای ارزیابی واکنش های فتوسنتزی است. مطالعه فلورسانس کلروفیل امکان تحلیل وضعیت مراکز واکنشی فتوسیستم II و کمپلکس های دریافت دریافت کننده نور را فراهم می آورد. این شاخص همبستگی بالایی با سایر پارامترهایی فیزیولوژیکی تحت تنش های مختلف محیطی دارد. شاخص کارایی فتوسنتز به عنوان شاخصی حساس برای ارزیابی تنش خشکی است به طوری که سطح مراکز واکنشی فعال در کلروفیل، واکنش های فتوشیمیایی اولیه و انتقال الکترون تحت تاثیر تنش خشکی قرار می گیرد. تحت تنش شوری میزان فلورسانس متغیر، فلورسانس حداکثر، انرژی لازم برای بسته شدن مراکز واکنشی و شاخص کارایی فتوسنتزی کاهش و در مقابل زمان لازم برای رسیدن به فلورسانس حداکثر افزایش می یابد. تحت تنش سرما بیشترین میزان جریان انتقال الکترون به ازای مراکز واکنشی، عملکرد کوانتومی فتوسیستم II، و کارایی کمپلکس تجزیه آب در فتوسیستم II کاهش می یابد. عناصر غذایی به ویژه پتاسیم مراحل وابسته به نور همچون اندازه آنتن های دریافت کننده و ارتباط الکترونی مراکز واکنشی فتوسیستم II را تحت تاثیر قرار می دهد. بخش گیرنده الکترون فتوسیستم II محل اصلی مهار انتقال الکترون فتوسنتزی تحت کاربرد علف کش ها است. ازاین رو، فلورسانس کلروفیل یک شاخص معتبر برای ارزیابی واکنش فتوسیستم II در شرایط تنش های محیطی است.

    کلید واژگان: تنش های محیطی, فتوسنتز, فتوسیستم II, فلورسانس}
    Ramin Lotfi *
    Introduction

    Plants in the environment are affected by various stresses, depending on the duration, intensity and growth stage of the plant, these stresses can reduce the process of photosynthesis and affect their growth and performance. However, traditional methods, even technically advanced ones such as the measurements of photosynthetic rates through the gas exchange (CO2, H2O, and O2), are time-consuming and provide incomplete information on overall photosynthetic function. The development of knowledge in the field of chlorophyll fluorescence shows that this indicator has a high ability to study the photochemical efficiency of plant photosynthesis.

    Methods

    For measuring the chlorophyll fluorescence in plants, leaves were dark-adapted for 30 minutes using leaf clips provided by the producer of handy-PEA. Measurements were performed on the middle of plant leaves following the standard protocol with illumination with continuous red light (peak in 650 nm wavelength; the spectral line half-width of 22 nm) provided by an array of three light-emitting diodes. The light pulse intensity used was 3500 μmol(photon).m–2s–1 and the duration of the light pulse was 1 s. The measured data were used for the calculation of the photosynthetic parameters using Biolyzer v. 3.06 HP software (a software provided with handy-PEA). Some of the parameters we discussed in this article due to their significance are FO = minimum fluorescence, FM = maximum fluorescence, FO/FM = The maximum quantum yield of basal non-photochemical energy losses, FV/FM = the maximum quantum efficiency of PSII, VJ = the relative variable fluorescence in step J after 2 ms, VI = the relative variable fluorescence in step I after 30 ms, N = the number of QA redox turnovers until FM, SM = the pool size of the electron acceptors on the reducing side of PSII, PIABS = performance index.

    Main Findings

    The study of chlorophyll fluorescence can analyze with high detail the function and state of PSII reaction centres, and light-harvesting complexes. This index has a high correlation with other physiological parameters under different environmental stresses. In this article, an overview of the results of chlorophyll fluorescence analysis of crops underenvironmental stressesis given, and the key steps to stresses are presented. Under drought stress the ratio of active reaction centers in chlorophyll, primary photochemical reactions, and electron transfer are affected. By salinity stress in crops, the values of variable and, maximum fluorescence, the energy required to close the reaction centers, and the photosynthetic efficiency index decrease, while the time required to reach the maximum fluorescence increases. Under cold stress conditions, electron transfer flow per reactive centers, the quantum performance of photosystem II, and the efficiency of the water splitting complex in photosystem II decrease. Potassium affects light-dependent steps such as the size of receiving antennae and the electron connection of photosystem II reaction centers. The electron acceptor part of photosystem II is the main site of inhibition of photosynthetic electron transfer under the application of herbicides.

    Conclusion and Implications:

    This article has provided an overview of the information about the wide opportunities of using the chlorophyll fluorescence technique in plant science, agriculture and ecological research. The measured parameters of chlorophyll fluorescence are called the JIP-test and its analysis can be used to evaluate the effects of environmental stresses on plants. This technique requires more practical studies in biotic and even non-biotic stress conditions to provide reliable information to investigate the growth and development of plants, and this leads to an increase in our knowledge of the physiological basis of crop photosynthesis under stress conditions.

    Keywords: Environmental Stresses, Fluorescence, Photosynthesis, Photosystem II}
  • سمیه میری، یاسر علیزاده*، حمزه علی علیزاده، اخلاص امینی

    به منظور بررسی اثر الگوهای کاشت بر خصوصیات فیزیولوژیکی ماش و ذرت در شرایط تنش خشکی، آزمایشی به صورت کرت های خرد شده در قالب طرح پایه بلوک های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشگاه ایلام طی سال زراعی 1401-1400 انجام گردید. کرت های اصلی شامل آبیاری در چهار سطح (40، 60، 80 و 100 درصد نیاز آبی گیاه) و کرت های فرعی شامل الگوی کاشت در چهار سطح (کشت مخلوط افزایشی 100 درصد ذرت+ 50 درصد ماش، کشت مخلوط جایگزینی 50 درصد ذرت+ 50 درصد ماش و کشت خالص ماش و ذرت) بودند. نتایج نشان داد تنش کم آبی (40 درصد نیاز آبی) باعث کاهش کلروفیل، سرعت فتوسنتز، مقدار رطوبت نسبی و سرعت تعرق در هر دو گیاه ماش و ذرت گردید. سرعت فتوسنتز ذرت در الگوی کاشت جایگزینی در مقایسه با کشت خالص ذرت 18.3 درصد بیشتر بود. غلظت کلروفیل ذرت در الگوهای کشت مخلوط جایگزینی و افزایشی به میزان 7.7 درصد بیشتر از کشت خالص ذرت بود. بیشترین غلظت دی اکسید کربن زیر روزنه، دمای برگ و مقدار پرولین برگ ذرت و ماش در شرایط 40 درصد نیاز آبی حاصل گردید. عملکرد دانه ذرت تحت شرایط 40 درصد نیاز آبی در مقایسه با 100درصد نیاز آبی به میزان 52.76 درصد کاهش یافت. بیشترین عملکرد دانه ذرت در کشت خالص ذرت و مخلوط افزایشی به دست آمد و کشت مخلوط جایگزینی کمترین عملکرد دانه ذرت را به خود اختصاص داد. در سطوح مختلف آبیاری بیشترین عملکرد دانه ماش از کشت خالص به دست آمد و عملکرد ماش در کشت مخلوط افزایشی و جایگزینی کاهش یافت. نسبت برابری زمین در همه الگوهای کاشت ذرت و ماش تحت شرایط آبیاری مختلف بیشتر از یک بود و نشان دهنده برتری کشت مخلوط ذرت و ماش بر کشت خالص بود. به طور کلی نتیجه گیری می شود که کشت مخلوط ماش و ذرت رهیافت مهمی برای افزایش تولید محصول در شرایط تنش کم آبی است.

    کلید واژگان: رژیمهای آبیاری, رنگیزه های فتوسنتز, سرعت فتوسنتز, کشت مخلوط, نسبت برابری زمین}
    Somayeh Miri, Yaser Alizade *, Hamzeali Alizadeh, Ekhlas Amini
    Introduction

    Under water stress conditions, intercropping can be used as a strategy for maximum use of sunlight and limited water resources. Due to the difference in morphological and physiological traits, the two plants corn and mung bean may be suitable for intercropping under drought stress; Therefore, this research was conducted with the aim of investigating the effects of drought stress on the physiological traits and yield of corn and mung beans in monocultures and intercropping in order to study the possibility of reducing water consumption in this production system.

    Materials and methods

    An experiment was carried out as a split plot based on randomized complete block design with three replications at Ilam University during 2019-2020 growing season. The main plot were four water regimes including (40, 60, 80, and 100% of the plant's water requirement) and the subplots were include the planting pattern at four levels (additive intercropping series 100% corn + 50% mung bean, replacement intercropping series 50% corn + 50% mung bean and monocultures of mung bean and corn). The application of drought stress started after the establishment stage of the plant and continued until the harvest. At the end of the growth period, some physiological traits of both plants were measured, including photosynthetic pigments, photosynthesis rate, transpiration rate, intercellular CO2 concentration, proline amount, leaf relative water content and grain yield. Finally, analysis of variance (ANOVA) was performed using Minitab 14 and SAS 9.1 softwares and the means compared by HSD test at 5% probability level (p≤0.05).

    Results and discussion

    The results showed the highest rate of photosynthesis (25.4 µmol CO2.m-2.s-1) was obtained in the treatment of 100% water requirement and the lowest rate of photosynthesis was obtained in 40% water requirement, which showed a decrease of 68.5% compared to the well-watered conditions. The rate of photosynthesis of corn in the replacement intercropping of 50% corn + 50% mung bean was 18.3% higher compared to monocultures corn. The highest rate of mung bean photosynthesis was observed in replacement intercropping under conditions of 100% water requirement and additive intercropping series under 80% water requirement, and the lowest rate of mung bean photosynthesis was observed in intercropping under conditions of 60 and 40% water requirement. Dehydration stress decreased the amount of total chlorophyll in mung beans and corn. The highest intercellular CO2 concentration (475.5 mmol of CO2.m-2.s-1) and the temperature of the corn leaf (36.7 °C) belonged to the 40% water requirement treatment. The rate of transpiration of corn and mung bean decreased by 55.56 and 61.43% in the condition of 40% water requirement compared to well-watered conditions. Water stress reduced the relative water content of corn and mung bean. The highest proline in corn and mung leaves (46.3 and 45.23 µmol.g-1.FW-1, respectively) was obtained in the treatment of 40% water requirement, which had no significant difference with 60% water requirement. Corn grain yield in the treatment of 40% water requirement showed a decrease of 52.76% compared to 100% water requirement. Monocultures (3945.8 kg.ha-1) and intercropping (3875.1 kg.ha-1) had the highest corn grain yield, and the lowest corn grain yield in replacement intercropping was 2700 kg ha-1. At all irrigation levels, the highest grain yield of mung bean was obtained in monocultures, and the yield decreased in additive and replacement intercropping, and additive intercropping had the lowest grain yield. The values of land equality ratio of all intercropping patterns were greater than one, which indicates the advantage of corn and mung bean intercropping patterns to use land and increase yield.

    Conclusion

    Considering the occurrence of recent droughts in arid and semi-arid regions of the country and the need for forage in these regions, the cultivation of forage plants, especially corn, is inevitable. Therefore, one of the appropriate strategies to protection in management and water consumption is the intercropping of this plant with plants of the legume such as mung bean. Intercropping can partially moderate the effects of drought stress on plant water conditions. In general, it seems that the patterns of intercropping and substitution, due to having higher grain yield potential, is a suitable strategy for producing higher yield and stability of this plant compared to monocultural under well-watered and stress water.

    Keywords: Intercropping, Irrigation, Land Equality Ratio, Photosynthesis Pigments, Photosynthesis Rate}
  • Sh. Sarikhani Khorami *, M. Dastfal, P. Salehi

    To evaluate and identify high yielding triticale lines syuiable to cold, temperate and warm agro-climatic conditions of Fars province, a field experiments were carried-out in Eqlid, Zarghan, and Darab agricultural research stations in 2012-2013 growing season. Twenty-nine triticale advanced lines (received from CIMMYT) were cultivated along with Juanilo 92 variety (check) in randomized complete block design with three replications. Each plot was four meters long with 1.2 meter in width. Fertilizers were applied based on soil analysis. The results showed that the highest grain yield (7450 kg ha-1) obtained from Darab and the lowest (2800 kg ha-1) from Eqlid. In Zarghan, Juanilo 92 variety had the highest grain yield (7300 kg ha-1). In Darab, the highest grain yield belonged to lines no. 2 and 21 with 8966 and 8900 kg ha-1, respectively. The results showed that the average grain yield of lines no. 8, 7 and 21 in all three locations were 1.8, 3.5 and 12.9 percent higher than Juanilio 92, respectively.

    Keywords: Triticale, Warm Climate, Temperate Climate, Cold Climate, 1000 Grain Weight}
  • احمد موسی پور گرجی*، داود حسن پناه، خسرو پرویزی، امیرهوشنگ جلالی، رحیم احمدوند

    به منظور بررسی سازگاری و پایداری عملکرد غده کلون های جدید سیب زمینی، 19کلون برتر انتخابی به همراه چهار رقم آگریا، مارفونا، ساوالان و خاوران در قالب طرح بلوک های کامل تصادفی با سه تکرار در دو سال 1394 و 1395 در پنج منطقه کرج، اردبیل، همدان، مشهد و اصفهان ارزیابی قرار شدند. تجزیه AMMI نشان داد، ژنوتیپ ها در محیط های مختلف واکنش متفاوت داشتند و مجموع مربعات اثر متقابل ژنوتیپ× محیط 4/8برابر بزرگتر از مجموع مربعات ژنوتیپ ها بود. کلون های,KSG81  ,KSG64  KSG300وKSG107  به همراه شاهد آگریا دارای پایداری عملکرد غده قابل قبولی بودند. تجزیه GGE Bi-plot مبتنی بر محیط نشان داد، مناطق اصفهان، اردبیل و همدان به ترتیب به منطقه ایده ال نزدیکتر بودند و کلون های ,KSG23 KSG82 ، KSG31 ،KSG64 و KSG300  به ترتیب با میانگین عملکرد 30/71، 29/76، 29/38، 27 و 26/68کیلوگرم در کرت (9 متر مربع) برای کشت در مناطق هدف مناسب تر بودند. ضریب برتری نسبی کلون های ,KSG23 ,KSG302 KSG81 و KSG107 به ترتیب 2/87، 6/98، 7/81و 12/49بود و از پایداری عملکرد غده خوبی برخوردار بودند. ضریب برتری نسبی کلون های KSG31 ,KSG82 و KSG64  به ترتیب 18/07، 15/76و 13/67و تقریبا مشابه شاهد آگریا (17/22) بود و از لحاظ عملکرد غده نسبت به شاهد آگریا برتری داشتند. براساس سازگاری و پایداری عملکرد غده، کلون های KSG302، KSG82 و KSG31 برای کاشت بهاره در مناطق مختلف کشور، کلون های KSG23 وKSG57  جهت کشت در خراسان، کلونKSG81 جهت کشت در اصفهان و اردبیل و کلون های KSG64 وKSG48  به ترتیب جهت کشت در همدان و اردبیل مناسب شناسایی شدند.

    کلید واژگان: سیب زمینی, تعداد غده در بوته, عملکرد غده کل, عملکرد قابل فروش, انبارمانی}
    A. Mousapour Gorji *, D. Hasanpanah, K. Parvizi, A. H. Jalali, R. Ahamadvand

    To study the adaptability and yield stability of 19 selected potato clones along with four cultivars, Agria, Marfona, Savalan and Khavaran a field experiment was carried out in randomized complete block design with three replications in five locations; Karaj, Ardabil, Hamadan, Esfahan and Mashhad, in 2015 and 2016. GGE Biplot based on environment scaling showed that Isfahan, Ardabil and Hamadan, respectively, were closer to the ideal location and KSG23, KSG82, KSG31, KSG64 and KSG300 clones with mean tuber yield of 30.71, 29.76, 29.38, 27 and 26.68 kg plot-1 (nine square meters), respectively, were more suitable for being grown in ideal areas. The relative superiority coefficient of KSG23, KSG302, KSG81 and KSG107 clones were 2.87, 6.98, 7.81 and 12.49, respectively, and they had relatively good tuber yield stability. The relative superiority coefficient of KSG31, KSG82 and KSG64 clones were 18.07, 15.76 and 13.67, respectively, and almost similar to the Agria control (17.22) and they were superior to the Agria (control) for tuber yield. AMMI analysis revealed that the genotypes reacted differently in different environments and the sum of squares of the genotype × environment interaction was 4.8 times larger than that of the genotypes. KSG81, KSG64, KSG300 and KSG107 clones, and cv. Agria (control) had reasonable tuber yield stability. Based on the adaptability and tuber yield stability, KSG302, KSG82 and KSG31 clones were identified suitable for spring planting in different regions of Iran, KSG23 and KSG57 for Khorasan, KSG64 and KSG48 for Hamadan and Ardabil, respectively, KSG81 for Isfahan and Ardabil.

    Keywords: Potato Clone, Quantitative, Qualitative Traits, Stability, Adaptability}
  • اسماعیل صفوی بختیاری، سید اصغر موسوی*، مهراب یادگاری، بیژن حقیقتی

    با توجه به گسترش کم آبی و لزوم شناسایی و معرفی ارقام متحمل به تنش خشکی، این پژوهش با هدف بررسی واکنش ارقام تجاری بادام به تنش خشکی به صورت کرت های خرد شده در قالب طرح بلوک های کامل تصادفی با سه تکرار در ایستگاه چهارتخته مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهار محال و بختیاری دو سال 1399 و 1400 اجرا شد. دور های آبیاری بر اساس درصد رطوبت قابل استفاده خاک بین ظرفیت زراعی تا نقطه پژمردگی شامل 70 درصد، 50 درصد، 30 درصد و 10 درصد رطوبت ظرفیت زراعی به عنوان کرت اصلی و پایه رویشی GN بدون پیوند و 13 رقم تجاری مامائی، ربیع، صبا، آراز، اسکندر، آیدین، شاهرود 6، 7، 8، 10، 12، 13، 21 که همگی بر روی پایه رویشی GN پیوند شده بودند به صورت تصادفی در کرت های فرعی قرار گرفتند. در هر دو سال مطالعه گیاهان به مدت چهار ماه از اول خرداد تا پایان شهریور تحت تنش آبی قرار داشتند و ارزیابی و اندازه گیری صفات مورد مطالعه در دو مرحله، اواسط دوره تنش (اواخر تیر) و اواخر دوره تنش (اواخر شهریور) دوره انجام شد. نتایج نشان داد که تنش خشکی موجب کاهش معنی دار وزن تر و خشک بخش های هوایی همه ارقام تجاری بادام شد و کمترین اثر تنش خشکی در رقم شاهرود 8 و پایه GN ثبت گردید. در هر دو مرحله مورد مطالعه اعمال تنش خشکی همراه با کاهش قابل توجه در شاخص کلروفیل بود و بین ارقام بادام نیز تفاوت معنی داری وجود داشت. در مقابل مقدار مالون دی آلدئید و نشت الکترولیت در همه ارقام بادام با افزایش شدت خشکی افزایش معنی داری داشتند. در هر دو مرحله اواسط و اواخر دوره تنش، ارقام شاهرود 8، شاهرود 12 و پایه GN بیشترین شاخص کلروفیل و کمترین مقدار مالون دی آلدئید و نشت الکترولیت را تحت تنش شدید خشکی (10 درصد رطوبت ظرفیت زراعی) را نشان دادند. فعالیت آنزیم های آنتی اکسیدانی کاتالاز، سوپراکسید دیسموتاز، گلوتاتیون پراکسیداز و پراکسیداز در طول دوره تنش در ارقام بادام روند صعودی داشتند. در شرایط تنش شدید خشکی بیشترین ظرفیت آنتی اکسیدانی از نظر فعالیت آنزیم های مورد مطالعه در ارقام شاهرود 12، شاهرود 8 و پایه  GN ثبت شد. براساس نتایج این پژوهش، در سطوح مختلف تنش خشکی، ارقام شاهرود 8، شاهرود 12 و پایه  GNبیشترین وزن تر و خشک، شاخص کلروفیل و فعالیت آنزیم های آنتی اکسیدانی را داشتند و در مقابل کمترین مقدار پراکسیداسیون در این ارقام مشاهده شد که بیانگر تحمل بالای این ارقام به تنش کم آبی بود.

    کلید واژگان: بادام, شاخص کلروفیل, دوره تنش, تنش اکسیداتیو, رقم متحمل}
    E. Safavi Bakhtiari, S. A. Mousavi *, M. Yadegari, B. Haghighati

    The present research was conducted to evaluate the response of different almond cultivars to drought stress. The experiment was carried out as split plot arrangement is in randomized complete block design with three replications in the Agricultural and Natural Resources Research Center of Chaharmahal and Bakhtiari, ShahreKord, Iran, in 2020 and 2021. Different irrigation periods based on the available soil moisture; 70%, 50%, 30% and 10% of field capacity were assigned to the main plots and 13 commercial almond cultivars; Mamaei, Rabie, Saba, Araz, Eskandar, Aidin, Shahrood 6, 7, 8, 10, 12, 13, 21 and GN clonal rootstock, were randomized in the subplots. All commercial almond cultivars were grafted on GN clonal rootstock. The plants were under water stress for four months and antioxidant enzymes activity was measured at the middle (two months after application of stress) and the end (four months after application of stress) of drought stress period. The results showed that drought stress significantly decreased fresh and dry weight of the above-ground parts of all cultivars. The lowest effect of drought stress was recorded in cv. Shahrood 8 and GN clonal rootstock. Malondialdehyde content and electrolyte leakage in all almond cultivars significantly increased with increasing drought intensity. In both the middle and end of drought stress period, cv. Shahrood 8, cv. Shahrood 12 and GN clonal rootstock showed the highest chlorophyll index and the lowest malondialdehyde content and electrolyte leakage under severe drought stress (10% FC). Under severe drought stress, the highest antioxidant capacity of enzymes was recorded in cv. Shahrood 12, cv. Shahrood 8 and GN clonal rootstock. Considering the results of this research, under different levels of drought stress, cv. Shahrood 8, cv. Shahrood 12 and GN clonal rootstock had the highest above-ground fresh and dry weight, chlorophyll index and antioxidant enzymes activity, and the lowest peroxidation level that showed the high level of tolerance to water deficit stress.

    Keywords: Almond, Chlorophyll Index, Stress Period, Oxidative Stress, Tolerant Cultivar}
  • شکوفه ساریخانی خرمی*، سیروس طهماسبی

    به منظور بررسی اثر تاریخ کاشت و ارتباط عوامل آب و هوایی در مراحل مختلف رشد با مراحل فنولوژیکی و عملکرد دانه برخی تجاری ارقام گندم نان، آزمایشی به صورت کرت های خرد شده در قالب طرح بلوک های کامل تصادفی با سه تکرار در سال های 99-1397 در ایستگاه تحقیقات کشاورزی داراب اجرا شد. کرت های اصلی شامل پنج تاریخ کاشت (پنجم و بیستم آبان، پنجم و بیستم آذر و پنجم دی) و کرت های فرعی شامل پنج رقم گندم (مهرگان، برات، خلیل، سارنگ و ستاره) بودند. نتایج نشان داد که ارقام دیررس تر خلیل و برات در تاریخ کاشت های زودهنگام عملکرد دانه بالاتری داشتند. ارقام زودرس ستاره و مهرگان در تاریخ های کاشت دیرهنگام، کاهش عملکرد دانه کمتری داشتند. بیشترین و کمترین میانگین عملکرد دانه ارقام به ترتیب در تاریخ های کاشت های 20 آبان (6934 کیلوگرم در هکتار) و پنجم دی (5775 کیلوگرم در هکتار) به دست آمد. تعداد روز و درجه-روز رشد (GDD) برای مراحل مختلف رشدی، با تاخیر در کاشت، کاهش یافت. این کاهش برای مراحل طویل شدن ساقه، گرده افشانی و رسیدگی فیزیولوژیکی از تاریخ کاشت اول به پنجم به ترتیب 181، 282 و 528 درجه-روز رشد بود. بررسی همزمان ارتباط عوامل آب و هوایی و اثر تاریخ کاشت بر خصوصیات ارقام، دو مولفه اول در مجموع حدود 40 درصد تغییرات را توجیه کردند. نتایج نشان داد که دما مهمترین عامل کنترل کننده در طول دوره های مختلف رشدی بود. با تغییر در تاریخ کاشت، درجه-روز رشد لازم برای مراحل مختلف مقدار ثابتی نبود که می تواند به دلیل اثر سایر عوامل محیطی باشد. نتایج این پژوهش می تواند به درک بهتر ارتباط عوامل آب و هوایی بر عملکرد دانه گندم نان و انتخاب ارقام سازگار برای منطقه داراب در شرایط تغییر اقلیم کمک کند.

    کلید واژگان: گندم نان, درجه-روز رشد, طویل شدن ساقه, گرده افشانی, رسیدگی فیزیولوژیکی}
    Sh. Sarikhani Khorami *, S. Tahmasebi

    This study aimed to investigate the effect of sowing date and enviromental factors during different growth stages on some phenological characterisitcs and grain yield of commercial bread wheat cultivars. The experiment was carried out as split-plot arrangements in randomized complete block design with three replications at the Darab reaesrch field station in 2018-19 and 2019-20 cropping cycles. Five sowing dates: October 27, 11, and November 26, December 11, and 26, were assigned to the main plots. Five commercial wheat cultivars: Mehregan, Barat, Khalil, Sarang, and Setareh, were randomized in the subplots. Results showed that the late maturity cultivars, Khalil and Brat, had higher grain yield in earlier sowing dates. Early maturity cultivars, Setareh and Mehregan, had less yield reduction in later sowing dates. The highest and lowest mean grain yield obtained from November 11 (6934 Kg ha-1) and December 26 (5775 kg ha-1), respectively. Number of days and required growing degree-days (GDD) decreased with delaying sowing date. The decrease of required GDD to reach commencement of stem elongation, anthesis and maturity stages from the first to fifth sowing date was 181, 282 and 528 GDD, respectively. Simultaneous effect of weather variables and sowing date on some characteristics revealed that the first two components explained approximately 41% of total variation. Results showed that temperature was the most important factor controlling the phenological stages duration. Despite the importance of temperature, changes in sowing date can alter the required GDD for phenological stages due to other environmental factors. In general, the results of this study can provide an insight into bread wheat cultivars responses to different climatic conditions and development of new bread wheat cultivars adapted to target environments.

    Keywords: Bread Wheat, Growing Degree-Days, Stem Elongation, Anthesis, Physiological Maturity}
  • مریم تاتاری*

    به منظور بررسی اثر سایه بان بر خصوصیات کیفیت میوه به رقم اصفهان در انبار، آزمایشی به صورت فاکتوریل در قالب طرح بلوک های کامل تصادفی در سه تکرار در باغ به شرکت کشاورزی فجر واقع در شهرستان نطنز استان اصفهان در سال1400-1399 به اجرا درآمد. هزار متر مربع از باغ مجهز به سامانه سایه بان و بخشی دیگر بدون سایه بان بررسی شد. میوه ها 193 روز پس از تمام گل برداشت شدند و به سردخانه با دمای 1±0 درجه سانتی گراد و رطوبت نسبی 95 درصد منتقل شدند. در زمان برداشت و نیز پنج ماه پس از برداشت و نگهداری در انبار، ویژگی های کیفیت میوه ها مورد بررسی قرار گرفت. محتوای پکتین و فنل کل پس از دوره انبارمانی پس از پنج ماه تفاوت معنی داری را بین تیمارهای زیر سایه بان و بیرون سایه بان نشان نداد، اما میانگین میزان مواد جامد محلول کل (TSS) (18/35درصد) و شاخص طعم (6/44) پس از پنج ماه انبارمانی در میوه های زیر سایه بان بیشتر بود. میزان کاهش وزن (6/41درصد) و قهوه ایی شدن (1/83درصد) در میوه های زیر سایه بان پس از انبارمانی کمتر بود. در بین عوامل و هوایی بررسی شده، شدت تابش در محیط زیر سایه بان (میانگین 11302/77لوکس) و بیرون سایه بان (میانگین 28881/10 لوکس) تفاوت مشهودی وجود داشت، به طوری که در بیرون سایه بان بیش از دو و نیم برابر زیر سایه بان بود. شاخص طعم بیشتر و نیز کاهش وزن و قهوه ایی شدن کمتر در انبارمانی میوه های تولید شده در زیر سایه بان از مزیت های کاربرد سایه بان در باغ های به است که کاربرد آن به باغداران پیشنهاد می شود.

    کلید واژگان: به, کاهش وزن, شاخص طعم, فنل کل, پکتین, تابش خورشیدی}
    M. Tatari *

    In this research, the role of shading net on some fruit quality characteristics of quince cv. Isfahan was investigated. The experiment was carried out on quince cv. Isfahan grafted on the Hawthorn rootstock in the quince orchard of Fajr Agricultural Company located in Natanz city in Isfahan province, Iran. Trees were about 20 years old. Fruits were harvested 193 days after flowering and were transferred to a cold store with temperature of 0±1°C and relative humidity of 95%. At the harvesting time and five months after harvest, the fruit quality characteristics were examined. The pectin content and total phenol content after five months of storage did not show significant difference between inside and outside of the shading net. However, the TSS (18.35%) and the fruit taste index (6.44) swere higher in the fruits under the shading net after five months of storage. Lower weight loss (6.41%) and browning (1.83%) were observed in the fruits under the shading net after five month storage.

    Keywords: Quince, Weight Loss, Taste Index, Phenol, Pectin, Solar Radiation}
  • Alireza Zebarjadi *, Lale Borjian, Hamidreza Ghasempour, Afsane Noori, Danial Kahrizi, Zeinab Chaghakaboodi
    As the demand for edible oil continues to soar, rapeseed cultivation remains pivotal for sustainable and efficient oil production, catering to the nutritional needs of a burgeoning population. Given the substantial impact of environmental stress on plant productivity, this study aimed to explore the in vitro responses of calli-derived hypocotyls from five Brassica napus genotypes under varying concentrations of polyethylene glycol (PEG) 6000 (0%, 10%, 20%, 30%, and 40%). The objective was to assess various growth and physiological parameters, including Callus Growth Rate (CGR), Relative Water Content (RWC), Index of Tolerance (INTOL), Relative Growth Rate (RGR), and Proline Content (PC). Results unveiled a significant surge in proline content with increasing levels of PEG, with the highest accumulation observed at 40% PEG concentration. These findings suggest that heightened osmotic stress induced by PEG led to a notable rise in proline accumulation in calli-derived hypocotyls. Furthermore, elevated levels of PEG-induced osmotic stress adversely impacted growth parameters such as RWC, RGR, and CGR. Notably, genotypes Geronimo and Arc5 exhibited enhanced drought resistance in in vitro environments compared to other genotypes. These particular genotypes showcased greater resilience and adaptability to PEG-induced osmotic stress, as evidenced by significantly higher measurements of relative water content (RWC), relative growth rate (RGR), and Callus Growth Rate (CGR). Physiological indices like Relative Water Content (RWC), Relative Growth Rate (RGR), and Callus Growth Rate (CGR) offer valuable insights into a plant's response to environmental stresses like water scarcity. This selection process is crucial for developing more resistant plant varieties that thrive even in challenging environmental conditions.
    Keywords: INTOL, PEG, Rapeseed, RWC}
  • Borzou Yousefi *, Roya Karamian
    Creeping savory is a wild plant that is used for comestible consumption, preparation of beverages, and production of sanitary ware and herbal drugs. To investigate the effects of salinity stress and salicylic acid on antioxidant enzymes, photosynthetic pigments, relative water content, proline, and soluble protein content in S. spicigera a factorial experiment was conducted based on a Completely Randomized Design (CRD) and three replications. The experiment was implemented at the greenhouse of Agriculture and Natural Resources Research and Education Center of Kermanshah, Iran (2019). Experimental treatments were four levels of salinity (0-50-100-150 mM NaCl) and two levels of salicylic acid (0 and 2 mM). Results showed that increasing salinity levels caused a significant reduction in relative water content, leaf fresh weight, leaf dry weight, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content. Salinity drastically enhanced the antioxidant activities (SOD, POD, and CAT), and cell proline content. Salicylic acid considerably decreased proline content under salt stress conditions, but improved antioxidant activities of SOD, POD, and CAT, and enhanced chlorophyll a, chlorophyll b, total chlorophyll, carotenoid content, protein content, relative water content, and leaf fresh weight under salt stress. Salicylic acid reduced the destructive effect of salinity on some morphological, physiological, and biochemical characteristics in creeping savory.
    Keywords: Antioxidant Activity, Chlorophyll, Proline, Salt Stress, Savory}
  • Lida Fereidooni, Zahra Tahmasebi *, Danial Kahrizi, Hooshmand Safari, Ali Arminian
    Water stress is one of the most important limiting abiotic factors for crop plants. The drought resistance was investigated for 35 camelina doubled haploid lines along with a check cultivar (Sohail), under two irrigation conditions (supplementary irrigation and rainfed), during the crop year 2020-2021. The study was conducted in the randomized complete block design by three replications in three regions of Eslamabad-e Gharb, Mahidasht and Mehrgan. The indices of stress sensitivity (SSI), tolerance (Tol), drought resistance (DTI), mean productivity (MP), geometric mean productivity (GMP), harmonic mean (MH), yield stability (YSI) and drought resistance (DI) were calculated using seed yield in two environments of rainfed (Ys) and supplementary irrigation (Yp). There was a significant difference among regions for YS, Yp, MP, GMP, MH, and DTI indices. MP, GMP, MH, DTI and DI indices had significant correlations with two environments. According to MH, MP and GMP, the highest and the lowest drought resistance were observed in the Mehrgan region and Islamabad region, respectively. Mahidasht showed the highest value of drought resistance and Mehrgan showed the lowest value of drought resistance among the lines based on DTI. In addition, the results of the mean comparison, cluster analysis, principal components analysis and the three-dimensional scatter diagram of the lines based on the yield of two environments and the MP index showed that lines 1, 2, 3, 5, 6, 10, 7 and the check cultivar had more drought resistant. These lines had high seed yield in two contrast environments and showed more drought resistance, which is recommendable for breeding programs in the climatic conditions of Kermanshah province.
    Keywords: Genetic Diversity, Oilseed, Seed Yield, Water Stress}
  • Ahmad Farid Rahmani, Mohammad Nazim Sakandari, Alireza Mirzaei *, Esra Uçar
    Stevia plant is one of the most important medicinal plants used to control diabetes due to its sweetening properties and low calories. Stevia is cultivated in many parts of the world, and to increase its sweetening properties, the effects of many different factors have been tested on this plant. In this research, we investigated the effect of elements related to metal oxidants on the induction of molecular levels and transcription. Thus, the activity of 3 key genes named CPPS, HDS, and GGDPS in response to six different metal oxidants named Cro3, Pbo, Fe2O3, Ag2O, Bao, and Tio2 was carried out in this research. The results showed that the increased concentration of metal oxides, especially Fe2O3 and TiO2, escalates gene expression in the biosynthesis of sweeteners extracted from stevia leaves. Also, related to all treatments, the higher the concentration, the higher the gene expression. Among all metal oxide treatments, Pbo and Bao resulted in low gene expression for CPPS, HDS, and GGDPS genes. On the other side, control showed the lowest expression regarding all three surveyed genes, indicating that using metal oxides can achieve higher production of sweeteners in stevia plants. The results of this research determined that physiological characteristics are affected by metal oxide treatments. Also, the expression of genes effective in the production of steviol glycosides, which is one of the important sweetening factors of this plant in the leaves, increases under the influence of these treatments. As a result, it can be said that the use of these treatments can have an increasing effect on the amount of sweetening of the plant.
    Keywords: CPPS, Gene Expression, GGDPS, HDS, Medicinal Plants, Metal Oxidants}
  • Saeedreza Vessal *, Narjes Amirchakhmaghi, Mehdi Parsa
    Water deficit stress is one of the key determinants causing crop yield losses globally. The present study was conducted to effectively screen Desi chickpea genotypes based on early dehydration tolerance-related traits as a tool for further evaluation in field experiments. Sixty-four genotypes of Desi chickpea were assessed under progressive water deficit stress, resulting in high variability in early growth characteristics and traits. The clustering analysis with UPGMA, separated the genotypes into three major groups, in accordance with biplot analysis grouping. The highest root length density was observed in the MCC438 genotype with an 18.6-fold increase compared to MCC884 which showed the lowest root length density among all analyzed chickpea genotypes. The genotypes MCC32 and MCC539 produced the higher shoot and root dry weight, while MCC884 showed the lowest value (with 12- and 32.5-fold differences, respectively). Ten genotypes showed differences in terms of their overall response to the water deficiency stress, including eight tolerant genotypes (MCC320, MCC418, MCC425, MCC438, MCC539, MCC540, MCC560, MCC576) and two susceptible ones (MCC433 and MCC897), were selected for further investigation of various growth and physio-biochemical traits based on drought response indices. A clear distinction was observed among ten analyzed genotypes for some physio-biochemical traits, indicating their tolerant responses to drought stress. Drought-tolerant candidate genotypes showed higher indices of seedling growth parameters, proline content, RWC, membrane stability, and root-to-shoot ratio in comparison to drought-susceptible candidate genotypes. The genotypes MCC425, MCC438, MCC418, and MCC539 were found more drought tolerant in the seedling stages, whereas genotype MCC433 was more sensitive. These results were consistent with what was obtained in our preliminary study. However, these results should be addressed further in the field conditions.
    Keywords: Chickpea, Drought Tolerance Index, Morpho-Physiological, Progressive Water Deficit}
  • معصومه نصیری، بابک عندلیبی*، سعید خماری، اسماعیل گلی کلانپا، سجاد نصیری

    گیاه لوبیا در بسیاری از کشورها از نظر رژیم غذایی در جایگاه ویژه ایی قرار دارد چرا که آن حاوی پروتئین و فیبر بالا بوده و منبع غنی از مواد معدنی ضروری بدن است. پژوهشی گلخانه ای با هدف بررسی اثرات بیوچار و اسید سالیسیلیک بر خصوصیات فیزیولوژیک گیاه لوبیا تحت شوری خاک به صورت آزمایش فاکتوریل در قالب طرح بلوک های کامل تصادفی در چهار تکرار در سال های 1401-1400 و 1402-1401 در دانشکده کشاورزی دانشگاه محقق اردبیلی اجرا شد. فاکتورها شامل بیوچار در چهار سطح (شاهد، بیوچار ساده و بیوچار تغییر یافته با اسید فسفریک و اسید سولفوریک)، محلول پاشی اسید سالیسیلیک در سه سطح (شاهد، 0/5 و 1 میلی مولار) و شوری در سه سطح 0، 4 و 8 دسی زیمنس بر متر) بودند. نتایج نشان داد که اثرمتقابل سه جانبه فاکتورهای آزمایش تاثیر معنی دار بر تمامی صفات مورد بررسی داشتند. بالاترین غلظت کلروفیل a و کاروتنوئید از ترکیب تیماری 1 میلی مولار اسید سالیسیلیک بدون کاربرد بیوچار در شرایط عدم تنش شوری خاک به دست آمد. با این حال کاربرد بیوچار تغییر یافته با اسید فسفریک بدون مصرف اسید سالیسیلیک در شرایط شوری خاک متوسط (4 دسی زیمنس بر متر) بالاترین میزان آنتوسیانین را در برگ لوبیا موجب شد. کاربرد یک میلی مولار اسید سالیسیلیک تواما با بیوچار ساده در شرایط عدم تنش شوری خاک، بالاترین میزان فتوسنتز جاری را در لوبیا موجب شد. در سطح شوری 8 دسی زیمنس بر متر خاک با وجود کاربرد اسید سالیسیلیک و بیوچار کمترین میزان فتوسنتز جاری به دست آمد. نتیجه گیری شد که تحت تنش شوری خاک، ویژگی های مختلف فیزیولوژیکی در گیاه لوبیا دچار تغییرات معنی دار می شود، اما کاربرد سالیسیلیک اسید و بیوچار می تواند به کاهش اثرات منفی تنش شوری خاک کمک کند. براساس نتایج کاربرد 0/5 میلی مولار سالیسیلیک به مراه بیوچار ساده در تنش شوری متوسط و کاربرد 1 میلی مولار سالیسیلیک اسید توام با بیوچار تغییر یافته با اسید فسفریک نتیجه بهتری نسبت به سایر تیمارها در اکثر صفات نشان دادند.

    کلید واژگان: آنتوسیانین, کلروفیل, فتوسنتز}
    M. Nasiri, B. Andalibi*, S. Khomari, E. Goli Kalanpa, S. Nasiri
    Introduction

    Phaseolus vulgaris L., commonly known as the common bean, is an important legume crop, responsible for 85% of global bean production. Nevertheless, soil salinity represents a significant challenge that severely impairs its productivity. Soil salinity represents one of the most detrimental constraints on crop growth and production. Conversely, beans are regarded as one of the most susceptible crops to salinity stress. However, the detrimental effects of salinity on crops can be significantly mitigated by the use of certain substances. Salinity exerts a significant negative influence on the primary plant processes, including photosynthesis and the production of photoassimilates, which ultimately results in a reduction in plant production. The application of an appropriate quantity of salicylic acid (SA) enhances the plant's tolerance to abiotic stresses, thereby reducing the destructive effects of stress and increasing tolerance to abiotic stresses. It has been demonstrated that the external application of salicylic acid can induce the tolerance of crop plants to a range of abiotic stresses, including salt, drought, heat, cold, and heavy metals. Furthermore, SA can be an effective substance against plant abiotic stresses, as it can regulate a variety of phytohormones, and it may play a key role in free radical scavenging and nutrient uptake. Furthermore, the utilization of biochar represents an efficacious approach to mitigating the consequences of abiotic stresses, such as salinity. Biochar, a carbon-rich material, is employed to enhance soil carbon sequestration, reduce CO₂ emissions, and augment soil microbial diversity and activity. It has been postulated that biochar has the capacity to adsorb some of the toxic ions, such as Na and Cl, thereby reducing the toxic effects of salinity.

    Materials and Methods

    A two-year glasshouse study was conducted using a randomized complete block design, with four replicates in each treatment. The study was conducted at the Faculty of Agriculture, University of Mohaghegh Ardabili, Iran over two consecutive years (2022-2023). The experiment included three levels of salicylic acid (SA): SA0 (0 mM), SA0.5 (0.5 mM) and SA1 (1 mM), four levels of biochar, including no biochar application as the control treatment (B0), recommended biochar (Rb) by application of 2.5% biochar. The treatments included 5% (w/w) of modified biochar with phosphoric acid (PA), 1.25% of modified biochar with sulfuric acid (Sb), and salt stress (SS) using NaCl in three levels: SS0 (distilled water), SS4 (4 dS m-1), and SS8 (8 dS m-1). In this experiment, the salinity treatment was applied in two stages: at the planting time and when the seedlings had fully established themselves in the soil. Salicylic acid was applied foliarly at two stages: early and late flowering, according to the predetermined levels.

    Results and Discussion

    Salinity had a detrimental impact on the biochemical attributes of bean plants, yet this effect was alleviated by the application of the SA treatment. Furthermore, salt stress resulted in a reduction in relative water content, membrane stability index, leaf area and photosynthetic pigments, which in turn led to a reduction in current photosynthesis and remobilisation of photoassimilates. Nevertheless, the application of biochar and the use of SA were found to alleviate the negative effects, resulting in a higher grain yield compared with the control plants, i.e. at the absence of biochar and SA application at the end of the experiment. The combined application of biochar and SA resulted in a higher relative water content, photosynthetic pigments and an enhanced rate of photosynthesis. The greatest accumulation of chlorophyll-a and carotenoids was observed in response to the application of SA1 without biochar in non-stress conditions. Furthermore, the lowest photosynthetic rate was observed in response to SS8. Moreover, the greatest anthocyanin accumulation was observed in response to the addition of biochar to the soil in the absence of SA under salt stress conditions.
    Our research has demonstrated that the combined application of biochar and SA can significantly mitigate the adverse effects of salinity stress on common beans. Furthermore, the results demonstrated that SA significantly enhanced grain yield of plants under both saline and non-saline soils. The results indicate that the excessive use of biochar and SA may lower the mentioned effectiveness, as the biochar increases soil porosity, which negatively impacts water availability. Furthermore, application of high doses of SA has been shown to have a negative impact on the biochemical and enzymatic attributes of the plant under salinity conditions.

    Conclusions

    Although the study on the effects of biochar and SA on common beans under salinity stress provides valuable insights, it is important to consider the limitations of the study. Consequently, the findings may not be directly extrapolated to the natural agricultural settings, where multiple environmental factors interact.

    Keywords: Anthocyanin, Chlorophyll, Photosynthesis, Relative Leaf Water Content}
  • ابوالمحسن حاجی امیری، عبدالرحمان محمدخانی*، رحمت الله غلامی

    این پژوهش به منظور بررسی روند تغییرات عناصر و مواد غذایی در سال های "آور" و "ناآور" زیتون طی سال های 1398و 1399 در ایستگاه تحقیقات زیتون دالاهو، در استان کرمانشاه انجام شد. آزمایش در قالب طرح بلوک های کامل تصادفی با سه تکرار و سه رقم زیتون محلی شامل دیره، زاگرس و مشکات انجام شد. اندازه گیری عناصر نیتروژن، پتاسیم، فسفر، روی، بور و مواد غذایی نشاسته و پروتئین از برگ، میوه جوان، برگ مسن و ریشه از زمان شروع سخت شدن درون بر، در اوایل خرداد تا زمان برداشت میوه در اواسط آبان، با فاصله زمانی هر40 روز یک بار انجام شد.بررسی نتایج نشان داد اختلاف بین تغییر محتوای عناصر غذایی در برگ و میوه جوان، نشاسته و پروتئین در برگ مسن و ریشه در سال های "آور" و "ناآور" معنی دار بود. به طوری که تغییرغلظت عناصر با الگوی منحنی رشد میوه (دابل سیگموئید) مطابقت داشت. میزان نیتروژن، پتاسیم، بور و روی در برگ جوان از اوایل خرداد تا اواسط تیرماه (زمان سخت شدن درون بر) کاهش و سپس جزئی افزایش یافت. میزان نشاسته در برگ مسن و ریشه در سال "آور" تا خرداد افزایش و سپس کاهش یافت. میزان نشاسته ریشه در سال "ناآور" از خرداد تا آبان با شیب کندی کاهش یافت. میزان پروتئین در برگ مسن و ریشه از یک منحنی دابل سیگموئید پیروی می کرد. به طوری که تا اواسط تیرماه با یک شیب تندی کاهش یافت. با توجه به تغییر غلظت عناصر در برگ و میوه مطابق با الگوی رشد میوه، مخصوصا در سال آور، کاربرد خاکی نیتروژن، فسفر و پتاسیم در دی ماه (قبل از گل دهی)، محلول پاشی برگی نیتروژن، پتاسیم، روی و بور در خردادماه (بعد از تشکیل میوه) و تغذیه نیتروژن و پتاسیم در مردادماه (پس از سخت شدن درون بر)  قابل توصیه است.

    کلید واژگان: الگوی رشد, تغذیه گیاهی, سال آوری, میوه}
    A. Hajiamiri, A. Mohammadkhani*, R. Gholami
    Introduction

    Olive (Olea europaea L.) is the most famous species of Oleaceae family that has edible fruits. The olive tree has a tendency to alternate bearing, leading often to the production of massive fruit yield in one year (on) and a very low fruit yield in the next year (off). Knowing the changes of nutrients in different stages of olive fruit growth and development in the “on” and “off” years provides the possibility of timely and correct application of fertilizers. This research was conducted in order to investigate the changes in the concentration of mineral elements, starch, and protein of olive fruit in the on and off-years.

    Materials and Methods

    This research was carried out in the years 2018-2019 at Olive Dalaho Research Station in 2018 and 2019 at Dalahu Olive Research Station in Kermanshah (Lat. 32° 30´ N, Long. 45° 51´, Alt. 560 m.), west of Iran. The experiment was carried out in the form of a randomized complete block design with three replications and three local olive varieties, namely Deira, Zagros and Meshkat. All tested 11-year-old trees were under similar conditions in terms of pruning, drip irrigation, fertilization and pest and disease control. Measurement were carried out on N, P, K, Zn, B, starch, and protein in young leaves, fruits, old leaves, and roots from the time of kernel hardening (in early June) until fruit ripening (in mid-November) every 40 days using both "on" and "off" year trees. The samples included 20 broad leaves from the middle part of one-year-old and newly-formed vegetative branches, the growing fruits of the current year, and the feeding roots of 30 cm depth under the canopy of the tree crown. Measurements on potassium concentration were made by a flame photometer (model PFP7 made by JENWAY, England), phosphorus by colorimetric method using spectrophotometer, nitrogen and protein by Kjeldahl method, boron and zinc elements using an atomic absorption device (Perking Elmer model 31/40). Starch concentration of the dry matter was measured by Morley's method.

    Results and Discussion

    Monthly changes of mineral elements in leaves and young fruits, starch, and protein concentration in old leaves and roots were significant in the on and off years. The monthly changes in the concentration of the mineral elements corresponded to the pattern of the fruit growth curve (double sigmoid). The concentration of N, K, B, and Zn in young leaves decreased from the beginning of June to the middle of July (the time of endocarp hardening) and then increased slightly. The monthly changes in concentration of mineral elements in leaves and young fruits, starch, and protein in old leaves and roots were significant in the on and off years. The monthly changes of the mineral elements corresponded to the pattern of the fruit growth curve (double sigmoid). The concentration of N, K, B and Zn in young leaves decreased from the beginning of June to the middle of July (the time of endocarp hardening) and then increased slightly. Mashkat cultivar had the highest and Deira and Zagros cultivars had the lowest concentration of nitrogen in young leaves. The concentration of nitrogen has a direct effect on vegetative growth, fruit formation, fruit yield, and shoot growth. There was a positive and significant relationship between fruit nitrogen and phosphorus and zinc concentration of the fruit, but young leaf nitrogen had a negative and significant correlation with phosphorus and zincof the fruit. Deira cultivar had the highest and Meshkat the lowest zinc concentration of the young leaves. Zinc plays a significant role in the function of enzymatic systems, synthesis of nucleic acids, protein and auxin metabolism. There was a positive correlation between phosphorus concentration in young leaves with phosphorus concentration in fruit and leaf starch, but there was a negative correlation with leaf and root protein concentration. The concentration of starch in the old leaves and roots increased and then decreased in the on-year until June. The concentration of root starch decreased slowly from June to November in off-year trees. The concentration of protein in old leaves and roots followed a double sigmoid curve, it decreased sharply until mid-July. From the mid-July to the end of August, the protein concentration of the old leaves slowly and strongly in old leaves and roots, respectively.

    Conclusions

    Considering the observed changes in the concentration of mineral elements in the leaves and fruits according to the fruit growth pattern, especially in the spring, soil application of nitrogen, phosphorus, and potassium in January (before flowering), foliar spraying of nitrogen, potassium, zinc, and boron in June (after fruit set) and nitrogen and potassium amendments in August (after endocarp hardening) is recommended for the olive orchards of the studied region.

    Keywords: Alternative Bearing, Fruit, Growth Pattern, Plant Nutrition}
  • هادی کشایی آرانی، محمد میرزاخانی*، کریم نوزاد نمین

    به منظور بررسی اثر کاربرد کودهای زیستی و آلی بر کارایی فیزیولوژیک نیتروژن و خصوصیات زراعی یونجه در منطقه کاشان آزمایشی در سال زراعی 93-1392 در شهر آران و بیدگل از توابع استان اصفهان، به صورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی با سه تکرار اجرا شد. فاکتورهای آزمایشی شامل سه نوع کود آلی (کود گاوی، کود گوسفندی و کود مرغی) به همراه شاهد هر کدام به میزان 10 تن در هکتار) و سه نوع حاصلخیزکننده زیستی (کود نیتروکسین، کود فسفات بارور-2 و ترکیب نیتروکسین و فسفات بارور-2) به همراه شاهد بودند. صفاتی مانند تعداد ساقه در مترمربع، تعداد دانه در مترمربع، تعداد روز تا ده درصد گلدهی، وزن هزار دانه، عملکرد علوفه تر، درصد نیتروژن، درصد پروتئین، عملکرد پروتئین، کارایی فیزیولوژیک و کارایی مصرف فسفر مورد ارزیابی قرار گرفتند. نتایج نشان داد که تیمار کاربرد تلفیقی کود زیستی نیتروکسین + فسفات بارور-2 با میانگین 68/4 تن بر هکتار نسبت به سایر سطوح کود زیستی از برتری 15/6 درصدی برخوردار بود. بنابراین می توان گفت که کاربرد توام نیتروکسین و فسفر بارور-2 توانسته است شرایط تغذیه ای بهینه تری را در چین برداری های متوالی نسبت به کاربرد منفرد هر یک از آنها ایجاد نماید. در بین سطوح کود آلی نیز مصرف 10 تن بر هکتار کود گاوی با میانگین 67/1 و عدم مصرف کود آلی با میانگین 62/3 تن بر هکتار به ترتیب بیشترین و کمترین میزان عملکرد علوفه تر یونجه را تولید نمودند. همچنین اثر متقابل کودهای زیستی و آلی بر اکثر صفات معنی دار شد و در بین اثرات متقابل، بیشترین میزان کارایی فیزیولوژیک نیتروژن با میانگین 3/40 کیلوگرم دانه تولید شده بر کیلوگرم نیتروژن مصرف شده مربوط به تیمار عدم تلقیح با کود زیستی + مصرف 10 تن بر هکتار کود گاوی بود.

    کلید واژگان: بارور-2, درصد پروتئین, عملکرد علوفه, فسفر, نیتروکسین}
    H. Keshaee Arani, M. Mirzakhani*, K. Nozad Namin
    Introduction

    Alfalfa is one of the most important fodder plants, being able to produce a high quantity of forage dry mass with high concentration of protein and to symbiotically fix atmospheric nitrogen. Having a deep and extensive root system, it reduces soil erosion and improves water penetration in agricultural soils (11). The results of the previous researches showed that the use of Nitroxin and Phosphate Barvar-2 bi-fertilizers had a significant effect on plant protein concentration and the highest protein (22.3%) was obtained when the plants were inoculated with Phosphate Barvar-2 and received organic amendment in the form of mushroom compost. Also, the use of Nitroxin and Barvar-2 bi-fertilizer increased the efficiency of nitrogen consumption, and inoculation with Nitroxin had the highest (22.8%) nitrogen use efficiency (5). Our study aimed to shed light on the nitrogen physiological efficiency and some agronomic traits of alfalfa in the presence of bio-fertilizers and organic manures in Kashan, central Iran.

    Materials and Methods

    This study was carried out in Aran and Bidgol, central Iran, in 2014. The experiment was conducted with a factorial arrangement in a randomized complete block design with three replications. Treatments were 4 levels of bio-fertilizers (control, Nitroxin, Phosphate Barvar-2, and Nitroxin+Phosphat Barvar-2) and animal manures (control, cattle manure, sheep manure, and poultry manure in 10 tons/ha). Stems/m2, grains/m2, 1000-grains weight, days to 10 percent flowering, accumulative fresh weight, nitrogen content, protein content, protein yield, physiological efficiency of nitrogen and nitrogen use efficiency were evaluated.

    Results and Discussion

    Application of bio-fertilizers enhanced all examined attributes, compared to control, the extent of enhancement in a majority of attributes being greater for Nitroxin + Phosphate Barvar-2. The highest and lowest grains/m2 and 1000-grains weight belonged to application of Nitroxin+ Phosphate Barvar-2 + 10 tons/ha cattle manure and control treatments, respectively. The highest nitrogen content, protein content, and protein yield were obtained by the application of Nitroxin and poultry manures. The highest physiological efficiency of nitrogen was recorded under the conditions of control and cattle manure (3.19 Kg/Kg). The highest nitrogen use efficiency was observed in the presence of cattle manure and Nitroxin+ Phosphate Barvar-2. The application of Nitroxin + Phosphate Barvar-2 with an average of 68.4 tons/ha led to 15.63% increase compared to other levels of bio-fertilizer. Therefore, the combined application of Nitroxin and Phosphate Barvar-2 has been able to produce a high-quality animal feed in successive harvests than the sole application of each of these bio-fertilizers. Among the different levels of organic fertilizers, application of cow manure with an average of 67.08 tons/ha and no use of organic fertilizer with an average of 62.37 tons/ha produced the highest and lowest cumulative fresh weight of alfalfa, respectively. Also, the highest physiological efficiency of nitrogen (with an average of 3.40 kg of grains produced per kg of nitrogen applied) was achieved when 10 tons/ha of cow manure was applied.

    Conclusions

    The combined application of Nitroxin and Phosphat Barvar-2 bio-fertilizers was more effective than the sole application of each of these biofertilizers. Among the levels of organic manure (10 tons per hectare), cow manure was found to be more effective in enhancing the examined traits. Application of poultry manure had a more favorable effect on nitrogen and protein concentrations and protein yield of alfalfa. Therefore, combined application of Nitroxin and Phosphat Barvar-2 bio-fertilizers is recommended for producing alfalfa in the climatic conditions of Kashan.

    Keywords: Barvar-2, Forage Yield, Nitroxin, Organic Manure, Phosphorus, Protein Percent}
  • جواد سرحدی، صابر حیدری*، مهری شریف

    عارضه زوال مرکبات تاکنون منجر به وارد آمدن صدمات شدید به باغات به ویژه در استان های جنوب کشور شده است. پژوهش حاضر در قالب بلوک های کامل تصادفی به مدت دو سال در جیرفت اجرا شد. تیمارها شامل 1. تیمار شاهد، 2. مدیریت بهینه (آبیاری، کود و به باغی)، 3. مدیریت بهینه به علاوه سیلیکات پتاسیم، 4 .مدیریت بهینه به همراه سایه بان و مالچ، 5. مدیریت بهینه به همراه قارچ میکوریزا و تریکودرما و سموم قارچ کش و نماتدکش، 6. تیمار 5 به همراه تزریق نیترات کلسیم و 7. تیمار 5 به همراه کود آبیاری نیترات کلسیم و عناصر میکرو به صورت محلول پاشی بود. تعداد شاخ و برگ جدید، مساحت برگ، عملکرد، طول و قطر میوه و غلظت برخی عناصر ماکرو و میکرو در برگ اندازه گیری شد. نتایج نشان داد تیمار شاهد دارای کمترین عملکرد و تیمار چهار (مدیریت بهینه به همراه سایه بان و مالچ) بیشترین عملکرد (50/2 کیلوگرم برای هر درخت) را به خود اختصاص داد. تعداد شاخ و برگ جدید (بهاره و تابستانه) در تیمار چهار، 191 عدد و در تیمار شاهد 76  عدد بود. در تیمار چهار میانگین سطح برگ 41/8 و در تیمار شاهد 29/4 سانتی متر مربع بود که 42/2 % نسبت به تیمار شاهد افزایش نشان داد. مقایسه آماری میانگین سایر پاسخ ها نیز نشان داد که تیمار چهار نسبت به سایر تیمارها افزایش معنی داری داشت. به نظر می رسد عارضه زوال در این منطقه ناشی از مجموعه ای از عوامل متعدد تنش های زنده و غیرزنده است. بنابراین اعمال مدیریت بهینه آبیاری و تغذیه تلفیقی همراه با استفاده از مالچ گیاهی در منطقه سایه انداز درختان و بکارگیری سایه بان می تواند به عنوان راهکار مناسب در بهبود عارضه زوال مرکبات در منطقه جنوب استان کرمان مورد توجه قرار گیرد.

    کلید واژگان: آبیاری بهینه, تغذیه تلفیقی, سایبان, نیترات کلسیم, مالچ گیاهی}
    J Sarhadi, S. Heidari*, M. Sharif
    Introduction

    Citrus decline is a significant deteriorating factor in citrus cultivation, imposing costly damages to many orchards worldwide, including in Iran. Key factors contributing to citrus decline include environmental stresses such as the physical and chemical conditions of the soil, soil nutritional status, soil moisture condition, and biotic stresses. Environmental stresses, particularly mineral deficiencies and water stress, appear to play a crucial role in citrus deterioration. The Jiroft region is one of the major agricultural centers in south of Iran. Despite its pivotal role in citrus production of the country, the region's orchards experience widespread deterioration, causing considerable annual losses. However, no research has yet explored the potential benefits of simultaneous use of management and nutritional treatments in these orchards.  Therefore, this study aims to investigate the effect of management and nutritional treatments on alleviating both biotic and abiotic stresses and reducing citrus fruit deterioration.

    Materials and Methods

    The current study employed a randomized complete block design over two years in Jiroft, south of Iran, incorporating the following treatments: 1. Control: No intervention. 2. Optimal Management: This included timely irrigation, fertilization (chemical and organic amendments based on soil and leaf tests), and gardening practices (pruning, fruit thinning, and weed removal). 3. Optimal Management + Potassium Silicate: This treatment received optimal management plus potassium silicate fertilizer applied through irrigation water three times at a rate of 30 L ha-1 (as determined by soil test). 4. Optimal Management + Shading: This treatment consisted of optimal management with shading (integrated or individual) at approximately 25% during the hot season, along with plant mulch. 5. Optimal Management + Biocontrol & Fungicides/Nematicides: This treatment combined optimal management with Mycorrhizal and Trichoderma fungi, along with fungicides and nematicides. 6. Optimal Management + Calcium Nitrate Injection: Trees received calcium nitrate injection (30%) directly into the trunk twice a year. 7. Optimal Management + Calcium Nitrate Fertilizer & Foliar Spray: This treatment involved optimal management with calcium nitrate fertilizer applied three times (500 grams per tree, one-month intervals) through irrigation water supplemented by foliar sprays containing calcium chelate and micronutrients (zinc, manganese, iron, and copper) applied three times. Then, leaf samples (both 5–6-month-old leaves and new leaves) and fruits were collected and transported to the laboratory for analysis. This analysis included measurements of number of new leaves, leaf area (average surface area per tree leaf), yield, fruit length and diameter, and the concentration of specific macro- and microelements in the leaves.

    Results and Discussion

    The results revealed an inverse relationship between the average number of new leaves per tree and the severity of decay. Treatment-4 displayed a 1.5-fold increase in new leaves compared to the control (treatment-1). Additionally, the number of final shoots (spring and summer regrowth) was 191 in the treatment-4 and 76 in the control treatment. In the treatment-4, the mean leaf area was 41.8 cm2, compared to 29.4 cm2 in the control treatment, demonstrating an increase of 42.20%.  Statistical comparisons of other responses also indicated a significant increase in treatment-4 compared to the others. Treatment-4 also exhibited the greatest improvements in fruit size. Fruit diameter and length increased by 35.5% and 33.9%, respectively, compared to the control. Treatment-4 resulted in the largest average leaf area (41.8 cm²) compared to the control (29.4 cm²). Fruit yield followed a similar trend, the lowest fruit yield (22.9 kg/tree) being observed in the control treatment. Conversely, treatment-4 (optimal irrigation management with shading and mulch) achieved the highest fruit yield (50.2 kg/tree), representing a significant increase of 119.2%, 45.9%, 52.5%, 2,100.0%, 95.3%, and 77.4% compared to control, treatment-2, treatment-3, treatment-5, treatment-6, and treatment-7, respectively. Treatment-4 also demonstrated the highest concentrations of zinc, manganese, and iron in the fruits. These elements increased by 1.50, 1.52, and 1.65 times, respectively, compared to the control. The results overwhelmingly favored treatment-4 compared to the control and other treatments. This superiority likely stems from the combined effects of shade mesh and mulch, which are unique to treatment-4. Shade mesh not only provides shade but also reduces radiation intensity, ultimately minimizing heat stress, sunburn, and water loss through evaporation and transpiration from the tree's foliage. Beneficial effects of Treatment-4 go beyond shade and mulch by ensuring optimal plant nutrition based on soil tests, addressing a crucial factor in citrus health. Inadequate nutrition can contribute to tree decline. By providing the necessary macro and micronutrients and adjusting environmental conditions like temperature and humidity, particularly around the roots, we can minimize disruptions in nutrient uptake and promote overall tree health.

    Conclusions

    Given the warm climatic conditions of Jiroft at Kerman's southern region, citrus decline arises from a combination of biotic and abiotic stresses. Consequently, solutions targeting either biotic or abiotic constraints are unlikely to provide a sustainable solution to the citrus decline question. An integrated approach, incorporating optimal irrigation and combined nutrient management, was found to be more effective. Therefore, implementing optimal irrigation and combined nutrition management, using plant mulch in the shaded area of the trees, and employing netting as a shade was proven to be a suitable solution to the citrus decline in the Jiroft at southern region of Kerman province, Iran.

    Keywords: Optimum Irrigation, Integrated Nutrient, Shade, Calcium Nitrate, Plant Mulch}
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