جستجوی مقالات مرتبط با کلیدواژه « Heterodera filipjevi » در نشریات گروه « زراعت »

Cereal cyst nematode (CCN) is one of the most deleterious pathogens of cereal crops worldwide including Iran. The species Heterodera filipjevi is widely distributed in the fields of wheat. Growing the resistant cultivars is a suitable environmental friendly and cost-saving procedure for nematode control. In current research, the resistance of 13 indigenous wheat genotypes and seven common cultivars of bread wheat to H. filipjevi were investigated in a completely randomized design with four replicates, under greenhouse conditions. Regarding to identification of the cyst nematode used in this study, the collected specimen was diagnosed through classical and molecular methods. Based on morphological and morphometric characters of cyst and second stage juvenile, amplification of rDNA-ITS regions and restriction fragment length polymorphism (RFLP), the studied cereal cyst nematode was identified as H. filipjevi. According to the analysis of variance, the effect of total numbers of cysts and females on the cultivars was significant at 1% level. The cultivars of Bezostaya and Katae, showed the highest and lowest mean total population in soil and root, respectively. Bezostaya showed susceptible reaction, katae, Lines 84 and 85 were grouped as resistant whilst, the other cultivars and lines showed moderately resistance reactions.

Cereal Cyst nematode is one of the most important damaging nematodes of wheat and barley in the world. Cereal cyst nematode Heterodera filipjevi has wider distribution in cereal fields in the country. In this study, the effect of mentioned nematode was evaluated on 13 wheat rainfed cultivars and root micro-morphological in greenhouse and field condition. H. filipjevi were identified based on morphometric and molecular characters. The number of cysts, second stage juveniles (J2) and eggs, reproductive factors and anatomical aspects of the root were analyzed. The results indicated that at greenhouse conditions, the lowest milky cyst was related to Zagros cultivar with 1.75 number and the lowest number of cereal cyst nematode eggs and larvae and reproductive factors were related to the Kohdasht cultivar with 1.32 and 0.26 number per gram of soil. Obtained results classified cultivars in two ranges of resistant and sensitive to cyst nematode. In the field, the lowest number of cyst was related to Zagros with 185.33 per 200 g of soil. The lowest number of CCN eggs and larvae and reproductive factors were related to Kohdasht with 3.76 and 0.68 per g of soil. Root components studies, the highest thickness of cuticle, periderm and parenchyma was observed for the Rasad, Karim and Rasad cultivars with an average of 12, 33 and 41.33 micrometers and the lowest was recorded for Karim, Rizhav and Aftab with an average of 6.67, 19.33 and 23 μm, respectively. Cluster analysis was also divided the studied cultivars in two different categories, which this results had a remarkable similarities with Duncan's test results.