جستجوی مقالات مرتبط با کلیدواژه « Tween 20 » در نشریات گروه « زراعت »

Microbial contamination (fungal and bacterial) of culture medium is one of the most common problems in plants in vitro micropropagation. In the present research, achievement of a reliable sterilization method, optimization of callus proliferation and plant regeneration medium in three Iranian commercial cultivars of sugarcane, CP48-103, CP69-1062 and CP57-164 were studied. For sterilization, 12 treatments were separately examined for each cultivar in a completely randomized design with 12 replications. In order to select the best callus culture and plant regeneration media, five treatments including MS medium supplemented with a combination of 2,4-D (2 mg l-1) and different levels of BAP and fifteen treatments using MS mediumcontaining different levels of auxin and cytokinin were studied, respectively in a completely randomized design with 10 replications.  Based on the results, disinfection treatments containing carbendazim 2 parts per thousand (15 min), 70% ethanol (50 seconds), 25% sodium hypochlorite (5% active chlorine) plus Tween 20 (15 min), and culture medium containing cefotaxime (200 ppm) and gentamicin (5 ppm) showed to be the best sterilization method without any contamination in CP69-1062 and CP57-164 cultivars and the lowest percentage of contamination in CP48-103. For callus culture, MS media containing 2,4-D (2 mg l-1) + BAP (0.5 mg l-1) exhibited the highest callus growth rate and proliferation. The highest percentages of plant regeneration were observed in treatments (Kinetin = 0.5 mg l-1 and BAP = 0.5 mg l-1), (Kinetin = 3 mg l-1 and NAA = 0.5 mg l-1) and (Kinetin = 3 mg l-1 and NAA = 0.1 mg l-1) with the means of 70%, 60% and 50% in CP48-103, CP57-164 and CP69-1062 cultivars, respectively.