جستجوی مقالات مرتبط با کلیدواژه "zanjan" در نشریات گروه "گیاهپزشکی"

Apple stem grooving virus (ASGV) is one of the important viruses infecting apple trees. In this research, 87 symptomatic and symptomless leaf and bark samples were collected from the apple orchards in west and south west of Zanjan and east of Kurdistan provinces to detect ASGV. After total RNA extraction from 50 out of 87 samples, cDNAs were synthesized by reverse primer and PCR optimized using a pair of specific primer corresponding to complete coat protein gene. A DNA segment with 728 bp length corresponding to complete coat protein gene was amplified from twelve samples by RT-PCR. Nine PCR-products including four samples cloned into pTG19 and five samples without cloning were sequenced. The sequencing and blast results revealed that the fragments belong to coat protein gene of Apple stem grooving virus. Phylogenetic analysis of new Iranian isolates and those retrieved from GenBank by MEGA 7 and Neighbor-Joining methods in Jukes-Cantor model showed that the new Iranian isolates were placed in a sub-clade with reported Iranian, Indian, South Korean and Chinese isolates. The result revealed that the least dN/dS ratio belonging to Iranian isolates and the most gene flow was calculated between isolates from Iran, China and South Korean. This is the first report of Apple stem grooving virus from the Zanjan region.

Description of Pratylenchus elamini Zeidan & Geraert, 1991 from IranManouchehr Hosseinvand, Shahin Ganjkhanloo and Ali Eskandari Our population identified by Geraert key (2013) as Pratylenchus elamini and agree well for most of the morphometrics and morphological aspects with the original description. This species has been originally described from Sudan by Zeidan & Geraert (1991). Rashno Bone Abbasi et al., (2018) only mentioned to Pratylenchus elamini name in list of identified species associated with citrus orchards in Dezful and Andimesk. In present study this species identified in rhizosphere of grass in Ijroud, Zanjan province and described for the first time from Iran.

Plant diseases caused by geminiviruses are one of the main constraints of legume production in the world. They are responsible for a constraint on production of various crops. Geminiviruses are characterized by their twinned icosahedral particles and their single-stranded DNA genome. The family Geminiviridae is grouped into four genera: Begomovirus, Mastrevirus, Curtovirus and Topocuvirus (Brown and Moriones, 2012). Recent reports for geminiviruses show that viruses such as Spinach curly top Arizona virus (SCTAV) and Beet curly top Iran virus (BCTIV) (Yazdi et al., 2008) can be grouped in a new genus, Becurtovirus (ICTV 2012;http://www.ictvonline.org/virusTaxonomy.asp). Zanjan Province is one of the main regions for growing bean in Iran. Various geminiviruses have been reported from bean included: Bean golden mosaic virus (BGMV), BCTIV, Bean Calico mosaic virus (BCaMV) and Bean dwarf mosaic virus (BDMV). In this study in order to determine the type and distribution of geminivirus/es causing bean diseases, samples were collected from Zanjan province and then tested for the presence of geminiviruses using polymerase chain reaction and rolling circle amplification systems.
Some of the geminiviruses cause disease in the bean. To identify geminiviruses in the bean, sixty samples were collected from Zanjan, Khdabadeh, KhoramDareh and Abhar in summer 2014. These samples were collected according to the symptoms such as golden mosaic, curling, malformation, blistering, yellowing of leaves and plant stunting. After DNA extraction, viral infection was tested by polymerase chain reaction (PCR) using degenerate primers, PAL1V 1978/ PAR1C 496 and Primer B/ Primer V181. Based on the disease symptoms and results of PCR, four samples were selected to confirm the presence of geminiviruses and also to amplify the full-length genome using a rolling cycle amplification (RCA) kit. The amplified DNA products were digested with EcoRI, PstI, and EcoRV enzymes. A 2800 bp DNA fragment was isolated from gel and cloned into EcoRI site of a pBlunt vector and then sequenced (Microgen, Korea). The resulted sequence was compared to other reported geminiviruses from databases such as Genbank. To find the phylogenetic relation of the identified virus with other reported geminiviruses, we made a phylogenetic tree using the aligned sequences in MEGA6 software by applying Neighbour-joining method with 1000 replicates.
Using degenerate primers more than 15 percent of the collected samples showed amplification of DNA fragments with expected sizes. Gel electrophoresis for PCR products using BC and PCR V181 primers for bean samples showed production of a 550bp fragment. The PCR products size was 900 and 1100 bp using PaR1C 496 and PAL1V 1978 primers. The phenotype for theses samples was included geminivirus-like symptoms such as: abnormal and yellowing (S37), cup shape (S60), yellowing and mosaic (S34), blistering and abnormality (S62). The same abnormal shape of leaves was reported from bean plants infected with geminiviruses such as BCTIV and BGMV (Gharouni K. S., 2012). According to the results of the PCR and type of the symptoms, four samples (S26, S37, S61 and S65) selected to confirm the geminiviral infection using Rolling Circle Amplification (RCA) reactions. The RCA reaction was performed using 200 ng of the DNA and followed the instruction of kit. The RCA product was digested with various restriction enzymes. Based on the digestion patterns of the amplified DNA in the presence of three restriction enzymes EcoRI, EcoRV, Pst1, a 2800 bp fragment from sample S37 was selected for cloning into a vector and sequencing. Analyzing of this sequence showed that the amplified DNA has the highest (98%) similarity to a Beet curly top Iran virus (BCTIV) isolated from sugar beet and a lower (89 %) similarity to an isolate of BCTIV from the bean in Khorasan-Razavi Province.
Geminiviruses are limiting factors for crop production in the bean. The most common geminiviruses are Bean golden mosaic virus and Bean calico mosaic virus. In Iran, BCTIV has been recently identified as a dominant and widespread curly top producing agent in important crops (Heydarnejad et al., 2007; Kardani et al., 2013; Soleimani et al., 2013). This diversity and wide occurrence of BCTIV made a big challenge for breeders to produce resistance or tolerant traits (Strausbaugh et al., 2008). Our results also confirmed the widespread occurrence of BCTIV in Iran and also the genetic variation of virus isolates from the same host plant in various geographical regions.