جستجوی مقالات مرتبط با کلیدواژه "تعیین جنسیت" در نشریات گروه "علوم دام"

The sex of chickens considerably impacts production performance and economic benefits in poultry farming. Male birds cannot lay eggs and usually have a lower ratio of meat to feed than broilers. Male chicks are typically killed immediately after hatching since they are redundant in the industry and male chicks will neither be suitable for egg production nor meat production. Day-old male chicks in the laying hen industry are usually culled immediately after hatching. As a result, this issue has caused moral concerns in societies. Efforts are underway to develop technology for automatically determining the sex of chick embryos, aimed at establishing a stable and efficient poultry farming system. In large commercial hatcheries, the sexing of newly born chicks is generally accomplished by three different methods according to new hatching lines' vent, color, or feathers. However, these methods are still time- and labor-consuming. If sex can be identified at an early embryonic stage or even before incubation, male eggs could be used as feed components. Moreover, fewer eggs would need to be incubated, which would reduce feed space requirements, CO2 emissions, and energy consumption, which are all economically beneficial to farmers and the environment. In recent decades, researchers have used various in ovo sexing strategies in chicken eggs before hatching or incubation. Some invasive and noninvasive studies that have been conducted for in ovo sexing of chicken eggs can be divided into five major categories: (i) molecular-based techniques, (ii) spectral-based techniques (Raman spectroscopy, fluorescent, 3D X-ray), (iii) acoustic-based techniques, (iv) morphology-based techniques, and (v) volatile organic compound (VOC)-based techniques. Commercially applicable methods must be noninvasive, rapid enough for real-time applications, economically feasible, and ethically acceptable. An alternative method, to prevent the removal of day-old chicks, is a non-invasive method to determine the sex of the egg in the early stages of hatching before the development of the nervous system. Recently, Raman spectroscopy was reported to determine the sex of eggs at the incubation stage. Raman spectroscopy is based on the Raman effect, whereby when incident light (wavelength 750–850 nm) excites molecules in a tissue, the molecules reflect light at a different wavelength. The reflected light's wavelength is characteristic of various chemical components and allows the detection of the atheromatous plaque chemical synthesis. Raman spectroscopy is a powerful tool expected to revolutionize chick sex determination because it can provide information about biological molecules. Thus, Raman spectroscopy is suitable for analyzing living organisms, leading to its widespread adoption across various biological and medical applications. Therefore, resonance Raman spectroscopies have found application in blood analysis, with some studies exploring its utility in chick sexing. For this purpose, the present study was carried out to investigate the feasibility of determining the sex of Iranian native chicken embryos using the Raman spectroscopy. To carry out this research, 100 fertilized eggs of Iranian native chickens were used. The sex of the embryos was determined using Raman spectroscopy with a wavelength of 785 nm during the fourth day of incubation. Validation of this method was investigated using the polymerase chain reaction (PCR) technique. Sequence alignment of CHD-Z and CHD-W allele sequences amplified by PCR technique. The amplified DNA fragments were single and double DNA bands in the size of 461 bp for the CHD-Z and 322 bp for the CHD-W genes. The PCR was carried out using a PCR master kit with specific primers (the forward primer: 5′- TATCGTCAGTTTCCTTTTCAGGT -3′, the reverse primer: 5′- CCTTTTATTGATCCATCAAGCCT -3′). Thermal cycling conditions for DNA amplification were: 1 cycle of initial denaturation at 94°C for 5 minutes; 35 cycles comprising 30s at 94°C for the denaturation, 30s at 59°C for annealing, 30s at 72°C for the elongation; and a final extension cycle at 72°C for 5 minutes. The PCR products were analyzed by electrophoresis on 2.5% agarose gel against a DNA Ladder 100bp, and visualized using the safe staining on UV transilluminator. The data obtained from the Raman spectrometer was analyzed using the Origin software. The main indices used to study the data were the intensity of the Raman peaks and the ratio of the dominant peak intensity. Additionally, principal component analysis (PCA) was employed to identify any patterns in the data. To calculate PCA1 and PC2, the ratios of I769/I838 and I1141/I1251 peaks were considered, respectively. PCA analysis can choose features that have a greater impact on the final result, depending on the data and the scope of their changes. The result of PCR showed that one fragment with a length of 461 bp was amplified for male embryos (ZZ) and two fragments with lengths of 461 and 322 bp were amplified for female embryos (ZW(. The study found that there are differences in the intensity of Raman bands between genders. Males have higher intensity while females have lower intensity. Therefore, changes in Raman spectra intensity can be used to identify gender. Additionally, the candlestick chart of the data showed that median and average values for males were larger than for females. Furthermore, the results obtained from PCA analysis showed that the variance percentages for PC1 and PC2 were 53.69% and 46.31%, respectively. PC2 is more reliable as it has less deviation. Based on the results obtained from the study, it can be concluded that Raman spectroscopy is a reliable method for determining the gender of chicken embryos during incubation. This test is quick, accurate, and can be easily incorporated into the industry to determine the gender of embryos without resorting to the practice of killing day-old chicks. Not only is this method more ethical, but it also offers a high level of accuracy, making it an attractive alternative for the industry. In general, these results demonstrate the potential application of hematological traits in developing an automatic in ovo embryo sexing method through spectroscopic analysis.

To date, sex determination technique in domestic animal has economic benefits. Recently, circulating free DNA (cfDNA) highlighted as suitable candidate for non-invasive sex determination in livestock and endangered wild life animals. Based on this motivation, the objective of this report was the potential usefulness of free fetal DNA in maternal blood for prenatal fetal gender determination in pregnant goats.

 In this respect, a total of 21 animals, including 17 pregnant females with a gestation period of six weeks, unborn goats and 2 males (negative controls) were selected. During the running experiment, estrous synchronization  was performed by intravaginal CIDR. The animals not returned to estrus was considered as an indicator of success in pregnancy. Also in the last months, ultrasound method was used to confirm pregnancy. In the next phase, cffDNA was extracted from the blood plasma of female goats using the available common experimental protocol. The primers in this experiment were designed based on AME gene located on X and Y chromosomes. The polymerase chain reaction was optimized based on available standard methods.

The observation addressed existence of two fragment in the male embryo (171 bp AMELX gene band and 111 bp AMELY gene band) and one fragment in the female embryo (171 bp AMELX gene band). Here, most of findings was in line of similar pervious literatures and sex determination based on AME gene in case of sensitivity, specificity and accuracy indices indicated 0.83, 0.93 and 0.93 respectively.

On this basis, the results opened a new horizon of the potential usefulness of free fetal DNA in maternal blood for prenatal fetal gender determination in pregnant goats.

The aim of this research was to utilize a simple, rapid, cheap and safe method for sex determination of newly born canaries through feather to help accurately breeding this bird. The external morphology of males and females of many birds such as canary are fairly similar to each other, Therefore, determining their sexes is very difficult. The different length of CHD (chromo-helicase-DNA-binding) gene in Z and W chromosomes can recignized by using molecular technique based and PCR method, for the sex determination of young birds. In this study, blood and feathers samples of 140 canaries from 10 different breeds were used and their DNA were extracted. It should be mentioned that for PCR, at first 5 primers designed and finally 1 specific primer were confirmed and used. The results showed that the amplification of a fragment with the length of 252 bp for males (ZZ) and two fragments with the lengths of 252 and 309 bp for female (ZW). The bands had a length difference of 57 bp. The DNA sequencing of these two fragments was done for the first time, the sequence of these two fragments of the CHD gene in canary species was submitted and accepted in the NCBI site under the names of CHDZ-1 and CHDW-1 with the accession numbers of MG679825.1 and MG679826.1.

Discovery of accurate, easy and economic embryo sex determination in the dairy cattle industry assumed highly particular importance. CffDNA is novel potential material to determine the sex of the fetus in early gestation period has not imposed negative effect on embryo health. In this regard, in overall, 16 Holstein cows experiencing eight weeks of gestation and 4 non-pregnant Holstein cows, two bulls (negative control) were chosen as candidates for this present experiment and subsequently that plasma was separated to extract ccfDNA. Qiagene commercial kit was used to extract. DEAD box protein gene-based primers for this study based on both chromosomes X and Y and past articles were selected and were synthesized reported. Polymerase reaction and the required conditions were optimized using proposed standard. The results of electrophoresis PCR patterns male fetus’s two bands and one band of female fetuses showed. Monitoring of birth of each gender embryos under the test after the birth of calves showed compelet match the molecular results re-confirmed