جستجوی مقالات مرتبط با کلیدواژه "خوکچه هندی" در نشریات گروه "علوم دام"

The current study intends to analyze the effects of methionine on the ovulation of guinea pigs. 60 guinea pigs were given 4 different treatments in a completely randomize design. The treatments contained 0، 2، 4 and 6 percent of methionine per kg as fed. After two weeks as adaptation period، the four different treatments were given to the animals for two estrus cycle. After synchronization which was achieved through the inter muscular injection of PGF2a analog (cloroprostonal). 6ml/kg of cloroprostonal was injected twice in an eleven-day interval. At the end of experiment guinea pigs were slaughtered and the numbers of mature follicles were calculated by loop microscopy. The result shown that، the number of mature follicle increased as the amount of methionine in the diet rased. The Duncan`s test showed a significant relationship between the number of follicle in the right and left ovary with the higher amount of methionine (P<0. 05) and didn’t reflect a significant relationship between weight of two ovary and the amount of methionine. It seems that if the amount of methionine increases in the diet، the ovulation will also increase regardless of the ovary and body weight.

Foot-and-mouth disease (FMD) is a severely contagious viral disease in cloven-hooved animals that it causes considerable economic losses in livestock productivity. Vaccination is one of the most effective procedures for control of FMD. One of vaccines performances is stimulating expression of some immune system genes، which called cytokines. In this study، expression changes of IFN-γ and TGFβ1 genes were evaluated in vaccinated guinea pigs with FMD type O inactivated vaccine. Blood samples were collected from vaccinated and control (no vaccinated) guinea pigs in three distinct times. After blood sampling، RNA was extracted and converted to cDNA. For measuring IFN-γ and TGFβ1 genes expression، relative Real-time PCR procedure was used. The results showed that expression of IFN-γ and TGFβ1 genes in the second and third blood sampling were significantly increased in comparison to the first blood sampling. Because increasing of cytokines expression is an indicative of the immune system response، these genes can be used as indicators for testing effects of the recombinant vaccines.

The pancreas contains both exocrine and endocrine components that secrete digestive enzymes and peptide hormones respectively. These two components are very different structurally and functionally but are intermingled within the gland. In the present study، the pancreases of 15 adult healthy animals (5 per each species) were used. After dissecting them، the pancreas of animals fixed in 10% neutral buffered formalin and then subjected to routine tissue processing for light microscopy. Different structures of the stroma and parenchyma of pancreas in hamster، guinea pig and rabbit were histomorphometrically studied using micrometrical method. All of parameters between three animals were analyzed and compared using ANOVA test. Results showed that the pancreas in three species was lobulated and compound tubulo-acinar gland having exocrine and endocrine portions. In the present study، the secretory acini of pancreas were oval or irregularly round and composed of 5 to 7 pyramidal cells arranged around a small central lumen. The mean percent of the islets of langerhans to the total pancreatic tissue in guinea pig (29. 8 ± 16. 04) was more than rabbit (9. 55 ± 4. 60) and hamster (10. 8 ± 3. 71). In hamster، diameter of islet was more than other animals. There was significant difference in diameter of islet of langerhans between animals (P<0. 001). It was observed 191. 30 µm، 41. 56 µm and 54. 60 µm in hamster، rabbit and guinea pig respectively.