جستجوی مقالات مرتبط با کلیدواژه « fatty acid » در نشریات گروه « علوم دام »

The aim of the present study was to investigate effect of additive genetic on egg weight, yolk weight and yolk fatty acid content and estimation of heritability for these traits. In this research, 150 white Japanese quails (50 males and 100 females) were used as the base population (without any pedigree information). In the F2 generation, 100 female quails were randomly selected at the fifth week of age and transferred to laying cages. The traits included egg weight, yolk weight and yolk fatty acid contents. (Co) Variance components and genetic parameters were estimated using multiple animal models by Wombat software. The estimated heritability for egg weight and yolk weight were 0.45 and 0.38, respectively. These estimates for yolk fatty acids ranged from 0.27 (Palmitoleic acid) to 0.45 (Palmetic acid). Genetic correlations between egg weight and yolk fatty acids were low, ranging from 0.01 (between egg weight and Linoleic acid) to 0.10 (between egg weight and Stearic acid). The higher genetic correlations were between yolk weight and yolk fatty acids, varying from 0.10 (between yolk weight and Linoleic acid) to 0.51 (between yolk weight and Stearic acid). The results showed that selection for increasing the yolk weight could lead to producing eggs with more contents of yolk fatty acids.

An experiment was performed to investigate the effects of using saturated and unsaturated fatty acids calcium salts on the production performance and some physiological traits of finishing lambs fattened under heat stress condition. Thirty-two cross bred Lori-BakhtiariÍRomanov lambs were randomly divided into 4 groups. Lambs were fed individually with the following experimental diets for 6 weeks (early July to mid-August): 1) Basal diet without supplemental fat (control), 2) Basal diet supplemented with 2% (on DM basis) of fish oil calcium salt, 3) Basal diet supplemented with 2% (on DM basis) of olive oil calcium salt and 4) Basal diet supplemented with 2% (on DM basis) of saturated fat calcium salt. Daily feed intake was recorded and lambs body weight was measured weekly. Rectal temperature and respiration rate were measured once per week. Using calcium salts of fish oil, olive oil and saturated fats in the diet significantly increased daily weight gain and reduced the lambs feed conversion ratio compared to control group (P≤ 0.05), but had no effect on dry matter intake of the Lambs. Regardless of fatty acid origin, supplementing lambs with calcium salts of fatty acids, reduced rectal temperature and respiration rate of lambs exposed to heat stress, compared to control group (P≤ 0.01). Also, using calcium salts of Fish oil, olive oil and saturated fat had no effect on DM, CP and EE digestibility, however, EE digestibility was higher in lambs consumed fat-supplemented diets than the control group. NDF digestibility was significantly higher in the control group lambs than the other groups. The results of this study showed that diet supplementation with calcium salts of unsaturated fatty acids of fish and olives and saturated fatty acids, improved the production performance of finishing lambs exposed to heat stress. No difference was detected between fat supplements with different fatty acid patterns, in this investigation.

In order to investigate the effect of partial replacement of cereal grains with different levels of calcium saltd of palmitic and stearic saturated fatty acids on the performance of fattening lambs, 30 male Dalagh-Romanov lambs were used for 89 days. Experimental treatments included: 1- control diet (without fatty acid), 2- diet with 3 % palmitic acid  (PA), 3- diet with 3% stearic acid (SA), 4- diet with 3 %(67% PA+ 33% SA), 5- diet with 3 %(67% SA+ 33% PA). Growth performance (dry matter intake, average daily gain and feed convertion ratio), nutrient digestibility and blood parameters (glucose, blood urea nitrogen, cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and liver enzymes) were measured. The results showed that experimental treatments had no significant effect on dry matter intake, feed conversion ratio and daily weight gain of lambs (P>0.05). Addition of fatty acid supplement to experimental diets significantly reduced dry matter digestibility (P<0.05). The concentration of high-density lipoprotein in diets containing fatty acid supplementation increased significantly (P<0.05). In conclusion, the results suggest that adding 3% )on the ration dry matter basis) PA and SA as alone or combine together in the diet for fattening lambs could be done without negative effects.

 Reproductive performance of domestic animals has a great impact on the profitability of the farms. It has been shown that several factors such as breed, age, season and nutritional management affect the quality of the produced semen and consequently fertility of rams. Among various nutritional factors influencing semen quality, fat has a great impact on both quantity and quality of produced spermatozoa so that its value is correlated with cell membrane fluidity, potent intracellular signal transduction molecules, and susceptibility to oxidative damage. It has been postulated that by participating in sperm plasma membrane fatty acids (FA), the ingested fat can change the ratio of polyunsaturated to saturated fatty acids (PUFA: SFA) and thereby improve several aspects of sperm quality. Also, lipids comprise a wide-range class of molecules that not only is served as a source of energy but also play a crucial role in the structure and function of spermatozoa. Dietary n‑3 PUFA supplementation has also shown to improve semen quality parameters in rams. Flaxseed oil contains up to 90% PUFAs, of which about 50% is α-linolenic acid. Several studies conducted during past decades indicate that dietary flaxseed supplementation improves sperm parameters of different species such as bovine, goat and rabbit.

Materials and Methods :

Fifteen mature 3-5 years old Kurdish ram weighing 65±2.5 kg (mean ±SE) were randomly allocated into three groups during 12 weeks of the experimental period. Animals were individually fed a standard basal diet supplemented with different levels of flaxseed. Treatment included FLS-0 (basal diet; control), FLS-5 (basal diet containing 5% flaxseed) and FLS-10 (basal diet containing 10% flaxseed. Blood and fresh semen samples were collected at weeks 1 and 12 of the experiment. The collected samples were examined for sperm concentrations, sperm motility, viability, acrosome integrity, host test and fatty acids profiles. The testis circumference was measured with flexible cloth tape. The largest circumference of the testes and both scrotum was measured after pushing the testes firmly into the scrotum. To measure plasma concentrations of glutathione peroxidase and testosterone, blood samples were collected from the jugular vein of all the rams at the beginning and after 1 and 12 weeks of feeding experimental diet.

Results and Discussion:

 Flaxseed supplementation did not affect testicles circumference, however, supplementation of flaxseed increased plasma concentrations of testosterone in (10% flaxseed) FLS-10 group compared to (5% flaxseed) FLS-0 (P<0.05). Treatment, time and their interaction did not affect glutathione peroxidase (GPX) activity. Semen concentration, proportion of live sperm, total motility and plasma membrane functionality was higher in FLS-10 compared to FLS-5 and FLS-0 groups (P<0.05). Flaxseed supplementation tended to increase percentage of sperm with normal acrosome (P<0. 01); however, percentage of abnormal was decreased in FLS-10 compared to the other corresponding groups (P<0.05). Treatment, time and interaction effect of treatment × time did not affect LIN, STR, ALH and VCL (P>0.05). However, BCF and VSL were improved in FLS-10 group as compared to the FLS-5 and FLS-0 group (P<0.05). A significant interactive effect of treatment × time was noted for VAP, where flaxseed supplementation did not affect VAP at first week of the experiment but higher VAP was recorded in FLS-10 compared to FLS-0 and FLS-0 groups after 12 weeks of flaxseed feeding (P<0.05). Flaxseed supplementation did not affect percentage of myristic, palmitic, stearic, oleic, linoleic, EPA and DPA, but the proportion of DHA, and the ratio of n-3:n-6 PUFA and SFA:PUFA was affected by the treatments (P<0.05). Dietary inclusion of flaxseed increased proportion of DHA and n-3: n-6 PUFA ratio and decreased SFA: PUFA ratio in FLS-10 compared to FLS-5 and FLS-0 (P<0.05). The interactive effect of treatment × time on DHA, n-3: n-6 and SFA: PUFA ratio revealed that there was no significant effect between treatment after first week of the experiment; however, proportion of DHA and n-3: n-6 PUFA ratio was higher and SFA: PUFA ratio was lower in FLS-10 compared to FLS-5 and FLS-0 (P<0.05). the significant correlations between addition of 10% flaxseed and most of the evaluated semen characteristics including live sperm, total motility, plasma membrane functionality, and acrosome status following 12 weeks of treatment feeding was in agreement with previous findings in male goat and rabbit. Other fatty acids concentrations, such as, linoleic acid, and docosahexaenoic acid (DHA) was improved by dietary flaxseed supplementation.

Conclusion:

 It can be concluded that adding 10% flaxseed to the Kurdish ram diet out of the breeding season can improve sperm quality. Sperm fatty acid composition can also be affected by dietary fat. But more research is needed to look at the effects of other flaxseed products, such as oil and powder. It is recommended that similar studies be conducted during the breeding season and with other amounts of flaxseed.

Improvement of the quality of animal products by new scientific and practical methods is one of the important goals of animal nutritionists. Rearing systems based on low access to pasture decrease the useful fatty acids in animal products. Several studies have been shown that feeding strategies are known as the most effective factor on alteration of fatty acid composition of muscle and fatty tissues in ruminants. Therefore, this study aimed to assess the influence of Pomegranate seed oil (PSO) with higher ratio of unsaturated fatty acids, steroidal and polyphenolic compounds on blood metabolites and fatty acid profiles in bicepsfemoris muscles in the fattening lambs.

Twenty one male Sanjabi lambs (BW=27.5±2.6 kg, 3-4 months old) were used to evaluate the effects of PSO supplementation on blood parameters and fatty acid pattern of bicepsfemoris muscle. Lambs were randomly distributed between three treatments (control, and diets containing 2 and 4% of PSO) and were fed adlibitum for 90 days. The experimental diets were formulated (DM basis) to be isonitrogenous (CP=14.7%) and isocaloric (ME=2.6 Mcal/kgDM) and to meet NRC (2001) .Blood samples were taken from each lamb (10 ml) at the end of first, second, and third months and metabolite parameters concentration in blood were measured. Samples of Bicepsfemoris muscle were dissected and homogenized for determination of fatty acid composition to the procedure described by Folch et al. (1957). Fatty acid derivatives were prepared by methylation of the fatty acids (Metcalfe and Schmitz, 1961). The pattern of Fatty acids were determined by using gas chromatography apparatus (17, 37). This study was based on completely randomized design. Data were analyzed statistically by general linear model (GLM) procedure and means comparing were done by Duncan's multi-domain test with SAS (2004) software version 9.1 (51).

Results show that concentration of AST, ALT and ALP, insulin, uric acid and creatinine, were not affected by different levels of PSO in diet. No significant difference was observed in the amount of palmitic, stearic, myristic, linoleic acid (C18: 2) and linolenic acid (C18: 3) in bicepsfemoris muscle. Addition of PSO at level of 4% decreased (P <0.05) the MUFA level compared to control (47.69 vs. 39.08). Mean PUFA and PUFA to SFA ratio in bicepsfemoris muscle of lambs received PSO were higher than those in the control group (2.3 and 2.2 times, respectively). However, these differences were not significantly different. Total amount of omega-3 fatty acids (PUFA n-3) significantly increased due to feeding PSO.

Due to positive trend observed in MUFA, n-3 fatty acids and PUFA: SFA ratio, with increasing the levels of PSO, addition 4% of this oil in the diet of fattening lambs is recommended.

The term “guinea fowl” is the common name of the seven species of gallinaceous birds of the family Numididae, which is originated from Africa and their meat and eggs are very popular. Eggs, one of the versatile foods, inexpensive and high nutritious, supply well balanced nutrients that affect human health. An egg is a rich source of protein and then have high biological value. Eggs contain essential proteins, fats, vitamins except vitamin C, minerals mainly including calcium, phosphorous, iron, magnesium, zinc, selenium, potassium and sodium, and bioactive compounds, and their compositions could be affected by type of bird, heredity, strain, age, hen diet, and environmental conditions such as season and rearing condition. An average weight of egg in commercial strains is usually 55 g and this trait mainly influenced by diet mainly the concentration of methionine and lysine and energy content. Distribution of proteins in egg component is equal between egg yolk and egg white. Other hands, the egg yolk is the major part of lipid, mineral and vitamins. The high consumer preferences of guinea fowl and its products demonstrate that guinea fowl is valuable and have high marketing potential. Compared to native hens and chickens, the guinea fowl’s benefits are: low production and rearing cost, high meat quality, greater capacity to scavenge for insects and grains, better ability to protect itself against predators and low incidence of common poultry diseases than chickens including parasites Newcastle Disease and Fowl Pox. In order to improving household protein supply and income and Because of the great potential for increasing guinea fowl production for smallholder farmers need to evaluate their products and specifically egg. This study was conducted to determine internal and external egg quality traits, chemical composition and fatty acid profile of yolk of East Azarbaijan province guinea fowl and compare their values with table hen eggs values.

A total of 50 clean eggs were obtained from Bonab guinea fowl research station by simple random sampling method. The guinea fowl breeders were at the same age and fed with similar diets. In order to compare of these values with table eggs values, 50 clean table eggs were purchased from a market. After sampling, the eggs were immediately transferred to the lab and internal and external egg quality traits were individually measured. Descriptive statistics of traits were determined using SAS software by univariate procedure and unpaired t-test was used to compare the data of guinea fowl eggs with table eggs data.

The results of this study showed that the mean external egg quality traits of guinea fowl eggs such as egg weight, shell ratio and thickness, egg shape index and egg volume were 40.736 g, 15.604% , 0.718 mm, 78.801 and 42.035 cm3, respectively. The mean of internal egg quality traits of guinea fowl eggs such as yolk and albumin percentage, haugh unit, yolk color, pH of yolk and albumin were 31.418%, 52.977%, 78.030, 8.80, 6.592 and 9.332, respectively. Also, the average external egg quality traits of table eggs such as egg weight, shell percentage and thickness, egg shape index and egg volume were 61.371 g, 9.080 %, 0.665 mm, 76.852 and 63.065 cm3. The mean of internal egg quality traits of table eggs such as yolk and albumen percentage, haugh unit, yolk color, pH of yolk and albumen were 31.334%, 59.585%, 75.075, 4.70, 6.431 and 9.415, respectively. The average of shell thickness of guinea fowl eggs were higher than hen eggs that indicate the shell strength of guinea fowl eggs is higher than table eggs. For determinating of chemical composition of guinea fowl and table eggs, 5 mixed samples (from each 10 eggs) were prepared. The amounts of albumin and yolk dry matter, albumin and yolk crude protein, yolk ether extract, and ash contents of shells were 13.526 and 46.662, 81.696 and 33.652, 31.456, and 89.924%, respectively and these values in table eggs were 12.730 and 45.770, 84.384 and 33.064, 30.406 and 77.564, respectively. The ash content of egg shells in guinea fowl was higher than that of table eggs. This is consistent with shell ratio and shell weight. Cholesterol content of the guinea fowl and table eggs were 1605 and 1391 mg/100 g of yolk, respectively. To determine the fatty acid profile of egg yolk of guinea fowl and table eggs, 5 mixed samples were selected and the fatty acids contents were measured by the gas chromatography method. In guinea fowl, the highest fatty acids content of egg yolks were palmitic acid, oleic acid and linoleic acid, with the amounts of 28.274%, 34.686% and 20.130% of total fatty acids of egg yolk, respectively. The highest and the lowest fatty acid content of table egg yolks were palmitic acid and docosapantaenoic acid with values of 29.176 and 0.026, respectively. The sum of saturated fatty acids (SFAs), monounsaturated fatty (MUFAs) acid and polyunsaturated fatty acids (PUFAs) in the egg yolk of guinea fowl were 38.144, 38.712 and 22.090 % and for table eggs were 40.638, 32.280 and 19.361 %, respectively. The sum of PUFAs of guinea fowl eggs significantly higher than table eggs (p<0.05). Sum of n-3 PUFAs in table eggs was higher than guinea fowl eggs (0.567 vs 0.342) and unlike n-6 fatty acids, the guinea fowl eggs had higher value in comparison of table eggs (21.746 vs 18.694). PUFAs and specially, n-6 PUFAs have a hypo-cholesterolemic effect than n-3 PUFAs and therefore guinea fowl eggs can decrease cholesterol content of serum.

According to the results, the level of cholesterol, n-6 fatty acids and n-6 to n-3 fatty acids ratio of guinea fowl eggs were higher than those of table eggs. Also, the sum of PUFAs and the sum of SFA to sum of MUFA ratio in yolk samples of guinea fowl eggs in comparison with table eggs were higher.

Studies have shown that the addition of oilseeds to ruminants diets can affect the fatty acid composition of livestock products. linseed oil contains more than 70% poly unsaturated fatty acids with alpha-linolenic acid generally contributing over 50% of total fatty acids. Hence, protection against rumen biohydrogenation determine the amount of alpha-linolenic acid reach to the duodenum. The extrusion of linseed may protect unsaturated fatty acids against ruminal biohydrogenation. However, the effects of extrusion on ruminal degradability of linseed are inconsistent in the literature. meta-analysis can combine the results of studies about linseed supplementation to the fattening lamb diets for accurate and reliable estimation.

A literature search was initially conducted using PubMed, Medline, Science Direct, and Google Scholar data bases and investigated references in the papers. It was also based on the following key words: fatty acid, linseed supplementation, linseed oil, growth performance, and fattening lamb. Then 12 studies were included in this meta-analysis; and prepared comparisons for average daily gain, feed intake and hot carcass weight and concentration of n3, n6 and conjugated linoleic acid fatty acids in subcutaneous adipose tissue and longissimus thoracis muscle. Meta-analyses were carried out using the Comprehensive Meta-Analysis package, version 3. The effect sizes of across studies were calculated with fixed and random effect models. The presence of true heterogeneity among studies was identified with Cochran’s Q–tests and quantification of the degree of heterogeneity was done with the I2 index. Possible publication bias was evaluated with funnel plot and statistical tests.

The results of this meta-analysis showed that the addition of linseed in the diet, has a positive effects on average daily body weight gain and hot carcass weight. However, linseed supplementation had no significant effects feed intake. Addition of linseed in the diet increase n3 fatty acids content and decrease n6 fatty acids concentration in subcutaneous adipose tissue and longissimus thoracis muscle, respectively. Subcutaneous adipose tissue and longissimus thoracis muscle conjugated linoleic acid concentration were not influenced by linseed supplementation. Comparison of the effect size of type of supplements to the overall effect size for the amount of n3 and n6 fatty acids in subcutaneous adipose tissue and longissimus thoracis muscle showed that the addition of flaxseed oil had the most effect on the concentration of this fatty acids.

The results indicate that feeding linseed increase growth parameters such as lambs average daily body weight gain, hot carcass weight and n3 fatty acids content in subcutaneous adipose tissue and longissimus thoracis muscle in fattening lambs. Nevertheless, adding linseed to diets reduced n6 fatty acids content in subcutaneous adipose tissue and longissimus thoracis muscle of lamb.

The aim of this study was to evaluate the effects of α- linolenic and linoleic fatty acids, trehalose and sucrose on quality of Markhoz goat frozen-thawed sperm cells. Semen was collected from 4 mature goats using an artificial vagina, evaluated, pooled and divided into 11 aliquots and extended 1:10 with basic tris diluent containing different fatty acids. The motility, progressive motility, viability, acrosome and membrane integrity of frozen spermatozoa have been evaluated after thawing. The results indicated that α- linolenic was significantly the best diluent, while the linoleic acid decreased significantly the quality of spermatozoa, except membrane integrity. Moreover, the use of trehalose and sucrose in diluents decreased significantly the sperm cells characteristics. In addition, the result presented that α- linolenic acid in combination with trehalose improved significantly the rate of acrosome and membrane integrity, but decreased the motility and progressive motility of frozen-thawed spermatozoa. Moreover, the treatments containing all antioxidants and diluents containing linoleic acid and sugars decreased significantly the rate of assessed parameters. In conclusion, the results in this study indicated that using of 20 mg α-linolenic acid in diluent alone and in combination with trehalose can be suitable for cryopreservation of Markhoz goat sperm cells.

The aim of this current study was using of a management and feeding program in order to improving of reproductive factors performance in one hundred head ewes with Iran Black bred. Ewes were placed in two A (75 head) and B (25 head) groups. Ewes of A group were milk-cutted and were introduced to rams 80 and 90 days after parturition respectively. Forty days before introduction to rams, melatonin implant was laid under skins of these ewes. 75 ewes of A group were kept separately in three groups of 25th and were fed by high starch diets complemented with saturated fatty (control treatment), extrude cotton seed (omega 3 treatment) and full fat soybean (omega 6 treatment). B group’s ewes were milk-cutted and introduced to rams 120 and 180 days after parturition and were fed the same as control treatment diet. Estrus, ovulation, lambing and twining rates were counted and recorded in various experimental treatment. The weight of lambs were measured in time of birth, 3th and 4th month. Lambing and twining rates was higher in omega 3 and omega 6 treatment than control treatment and B group. Lamb weight average in their 3 and 4 month old was significant higher in B group than all treatment of A group. Generally, results of this study showed that using of management program of A group along with omega 3 and 6 feeding could improve reproductive factor performance.

Introduction Poultry meat enriched with long-chain polyunsaturated fatty acids n-3 (PUFA Lc n-3) can make a nutritionally meaningful contribution to Western diets in which consumption of PUFA Lc n-3 is low. Enrichment of poultry meat with this fatty acid is usually achieved by inclusion of fish oil in broiler diet (23, 24). However, meat enriched in this way is susceptible to quality deterioration by lipid oxidation during storage or cooking, leading to reduction in nutritive value and accumulation of lipid oxidation products (10). Oxidation is a very general process affecting lipids, pigments, proteins, DNA, carbohydrates, and vitamins (11). The objective of this study was to evaluate the effects of dietary α-tocopherol (α-Toc), pomegranate peel extract (PPE) and pomegranate peel (PP) on fatty acid profile, aoxidation and phenolic compounds in raw thigh and breast meat during refrigeration.
Materials and methods Peels of pomegranate were harvested in October 2011 from pomegranate trees (Ardestani, variety) in Khorasan Razavi province (East, Iran). Dried powders of peels (2.5 g) were extracted with 40 mL of methanol solvent at room temperature for 6 hours. Three hundred and eighty four 1-d-old male broiler chicks (Ross 308) were randomly allotted to 8 groups with 4 replicates of 12 birds. Eight dietary treatments including control diet without feed additives, control diet mixed with 200 mg/kg α-Toc, control diet mixed with PPE (100, 200 and 300 mg/kg), and control diet mixed with PP (1, 2 and 3 g/kg). In all diets 2% fish oil were added to enhance the enrichment of unsaturated n-3 fatty acid in birds. One broiler chick was randomly selected from each pen of 42 d of age. The antioxidative potential and various meat quality characteristics were determined on 0, 7, and 11 days of refrigerated storage. Total phenols content in the aqueous supernatant was estimated by the Folin-Ciocalteu method (33). 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was estimated according to Blois’ (2). Lipid oxidation in sample was determined as the TBARS value by using the method described by Ahn et al. (1). Fatty acid profile of samples was determined by gas chromatography (7). Data were subjected to ANOVA using the GLM procedures of SAS (27).
Results and discussion The FA composition of the diets, particularly PUFA Lc n-3, was modified and increased by the inclusion of fish oil in diet. The concentration of saturated fatty acid in the thigh and breast meat was not influenced in broilers fed PP, PPE, and α-Toc diets as compared to control group. The results indicated that the tissue of broilers have a limited capacity for alteration of their saturated fatty acids (7). Monounsaturated fatty acid (MUFA) content in thigh and breast was decreased in birds fed diet containing PP, PPE and α-Toc compared to control group (P

The aim of this study was to evaluate the effect of use of different levels of Citrus Limon powder on blood metabolites concentrations, rumen fermentation and apparent total tract digestibility of nutrients in male Makuii sheep. This study were conducted using Three male Makuii sheep, each fitted with a permanent rumen cannula (average body weight 55 ± 5 kg) that received experimental diets including control without additives, control with 5, 10 and 15 mg/d Citrus Limon powder and control with 30 mg/d monensin in a change-over complete randomized block design with 5 periods of 17 days. The effect of treatments was not significant on digestibility of dry matter, organic matter, acid detergent fiber, ether extract and crude protein. But neutral detergent fiber digestibility of control treatment were more than the other treatments (P<0.05). Results of this experiment showed that different levels of Citrus Limon powder decreased acetate, ammonia N, acetate to propionate ratio and increased propionate concentration (P<0.05). Also concentration of total volatile fatty acid was different in experimental treatments. There was no significant difference between the treatments in valerate, iso-valerate and butyrate concentration and pH values. Effect of different of experimental treatments on blood metabolites such as glucose, cholesterol, triglyceride were significant. In conclusion, results showed that utilization of Citrus Limon powder in high levels could improve ruminal fermentation.

This experiment was conducted to determine the effect of supplemental Cu on rumen parameters and carcass composition of male lambs. Twenty four 3–4 month old male Lori-Bakhtiary lambs were used in this experiment. The lambs were housed in individual pens and assigned randomly to one of three treatments. Treatments were as follows: 1) without supplement (control), 2) lambs received 5 mg Cu/kg DM, and 3) lambs received 10 mg Cu/kg DM. Additives were fed before AM feeding. Feed offered and refusals were recorded daily prior to the morning feeding. Body weight was measured every two week of experiment. Also rumen fluid was sampled to evaluate the parameters. At the end of fattening period, lambs were slaughtered and carcass characteristics were measured. Results showed that addition 10 mg Cu/kg DM to feed lambs was reduced significantly feed efficiency and average daily gain (p<0.05). Additives Cu had no significant effect on DMI and final body weight (p>0.05). Also, body weight, and dressing percentage were not affected by supplementations (p>0.05). Lambs received 10 mg Cu/kg DM had lower back fat versus others (p=0.04). Levels of copper added did not affect on profile of fatty acids. Supplement Cu had no effect on rumen fluid ammonia nitrogen, but with the addition of high levels of Cu was tend to reduced in the rumen fluid pH (p=0.07). Also, VFA concentration in rumen fluid samples was not affected by the add-Cu (p>0.05). The strong inverse relationship numerical was between the Cu level and total VFA in rumen fluid. Based on the results of this experiment supplement Cu sulfate in rang of 5 to the 10 mg Cu/kg DM could affect rumen microorganism’s activities and fat production of the lambs.