جستجوی مقالات مرتبط با کلیدواژه "homocysteine" در نشریات گروه "علوم دام"

This experiment was performed to compare the absorption kinetics and utilization of different methionine (Met) sources including synthetic-free crystalline DL-Met (DL-Met), L-methionine (L-Met) and dipeptide (Met-Met) and protein-bound methionine (PB-Met) in broilers with single-meal feed consumption. A total of 100 female Ross 308 broiler chickens were fed in a completely randomized design with five experimental diets (basal, DL-Met2.0, L-Met2.0, Met-Met2.0 and PB-Met2.0) in accordance with compliance to the single-meal broiler breeder from 39 to 60 days of age. During experimental period, 90 grams of pellet feed was given per chicken once a day, which was consumed within 17±2.5 minutes. The results showed that dietary supplementation with PB-Met2.0 caused a significant improvement (P<0.01) in growth performance, compared to the consumption of synthetic amino acids. The maximum blood plasma methionine concentration was observed at 1 hour after consumption of diets containing DL-Met2.0 and Met-Met2.0 and 2 hours after consumption of diets containing PB-Met2.0 and L-Met2.0 (P<0.01). The highest carcass protein content and protein utilization efficiency (P<0.01) and also the lowest excreta nitrogen content (P<0.05) were observed in treatment containing PB-Met2.0. The efficacy of essential amino acids, especially methionine was increased in chickens fed diet containig PB-Met (P<0.01). As a result, it seems that DL-Met and Met-Met have less efficiency in broilers with single-meal feed consumption, due to the lack of absorption synchronization with other amino acids. Therefore, the absorption synchronization of methionine using appropriate sources increases its efficiency. In conclusion, PB-Met is recommended as a preferred source of methionine in diet of broilers with single-meal feed consumption.

The present study aimed to investigate the effect of dietary betaine supplementation on circulating homocysteine, antioxidant enzymes and lipid peroxidation in pregnant Sanjabi ewes. Twenty multiparous pregnant ewes (3-5 years old) were fed either a basal diet (Control: 71.2±3.6 kg BW) or the basal diet supplemented with five g per kg dry matter betaine (Betaine: 71.6±3.8 kg BW) during the last five weeks of gestation in a completely random design. Feed intake, body weight (BW) and body condition score (BCS) were weekly recorded. Blood samples were taken from the jugular vein at 28, 14, seven, and one-day pre-partum. The activity of glutathione peroxidase (GPx), catalase (CAT) and blood concentration of homocysteine, and malondialdehyde (MDA) were determined. Dry matter intake, BCS, and BW of ewes were not affected by betaine consumption. In both groups, the activity of GPx increased (P<0.05), while CAT and MDA decreased (P<0.05) as gestation progressed. The activity of GPx and CAT remained unchanged by betaine. Ewes receiving betaine showed greater (P<0.05) blood homocysteine, but lower (P<0.05) MDA as compared to the control ewes. The birth weight of lambs in Betaine ewes was heavier (P=0.06) than those of control ewes. In conclusion, dietary supplementation of betaine was beneficial for alleviating oxidative stress and improving lambs' birth weight in Sanjabi ewes.