جستجوی مقالات مرتبط با کلیدواژه "microsatellites" در نشریات گروه "علوم دام"

Totally, 3200 and 320 young worker bees were investigated morphologically and molecularly respectively. The four important honey bee microsatellite loci, A24, A28, A29, A113, indicated a considerable level of genetic diversity among the nation’s northwest honey bee populations and, considering average number of alleles per locus (3.25-5.75) and, average expected heterozygosity (0.445-0.701) high level of variability was shown. Moreover, according to molecular analysis of variance (AMOVA), intra-population and inter-population genetic diversity were estimated to be 70% and 30% respectively. In addition, statistical analysis of morphological characteristics using Principle Component Analysis (PCA) revealed that northwestern honey bee population of Iran could be divided into, at least, three groups. Considering the high genetical diversity of honey bee in northwest Iran, establishing queen rearing stations and executing interbreeding programs for honey bee population in northwest Iran seems to be necessary. Furthermore, considering the high level of polymorphism in the studied markers, they are recommended to be applied for Quantitative Trait Loci (QTL) mapping, Marker Assisted Selection (MAS) and, other related investigations.

Assessing genetic biodiversity and population structure of minor populations through the information provided by neutral molecular markers, allows determination of their extinction risk and to design strategies for their management and conservation. Analysis of microsatellite loci is known to be highly informative in the reconstruction of the historical processes underlying the evolution and differentiation of animal populations. Iranian goat populations are recognized as an invaluable component of the world’s goat genetic resources. The aim of this work is to study the genetic status of Raeini goat population through the analysis of molecular data from 13 microsatellites markers. The mean expected heterozygosity across loci within populations ranged from 0.716 to 0.852. Genetic diversity measures revealed a good status of biodiversity in the Raeini goat population. The observed number of alleles ranged from 4 (ILSTS022) to 10 (BM121) with a total of 92 alleles and mean of 7.08 alleles across loci. The overall heterozygosity, PIC and Shannon index values were 0.808, 0.76 and 1.719 indicating high genetic diversity. Only 5 out of 13 loci were in Hardy-Weinberg equilibrium. The mean Fis was -0.016. Only 4 loci had positive Fis values and 9 loci had negative values. Genetic bottleneck hypotheses were also explored. Our data suggest that the Raeini goats have not experienced a genetic bottleneck in the recent past.