جستجوی مقالات مرتبط با کلیدواژه « استخراج پروتئین » در نشریات گروه « شیلات »

The purpose of this research was to investigate different methods of protein extraction and compare and choose the best method for the functional properties of protein from spirulina microalgae. Microalgae are one of the most important sources of protein. Proteins play a very important role in human health and are widely used in various industries. . In this research, different methods (freezing-thawing, homogenization, ultrasonic waves and soaking in ethanol and water solutions) were investigated to extract and isolate protein from spirulina microalgae. The functional properties of extracted proteins (water retention capacity, foam production and stability, and emulsifying capacity of the samples) were investigated. According to the results, the extraction method had an effect on the functional properties of the protein (p<0/05). According to the obtained results, the use of mechanical methods for protein extraction resulted in better preservation of functional properties. The functional properties of protein (Water holding capacity, emulsion stability index, foam stability, foam ability, samples extracted with the help of homogenization, ultrasound, freezing and thawing methods were more than other treatments (p<0.05). The index of emulsification capacity and water holding capacity in samples extracted with 4 freeze-thaw cycles was higher than all treatments. The highest level of emulsion stability and foam production was in the samples extracted with ultrasonic waves. According to the results, the use of ultrasonic waves and freeze-thawing cycle is suggested for protein extraction from spirulina microalgae.

Fish epidermal mucus protein extraction for bioassay-guided screening is challenging and variable. In this study, four different protein extraction methods, including acetone, trichloroacetic acid (TCA)-acetone, methanol, and solid-phase extraction (SPE), were investigated to study their efficiency in ultrafiltration and to obtain the appropriate peaks in HPLC to measure biological activities. The presented data shows that the solid phase extraction method is better than the other three tested methods in quantity (total protein yield) and quality (protein profile observed in SDS-PAGE) of proteins and peptides from the epidermal mucus of Caspian monkey goby. On the other hand, due to the epidermal mucus hydrophobic nature, all three extraction methods including acetone, (TCA)-acetone and methanol led to the irreversible proteins aggregation, makes ultrafiltration, HPLC, and consequently bioactivity screening impossible. In this study, the highest amount of protein (4.676 μg/ml) (P<0.01) and the best protein profile (13 bands) were related to solid phase extraction. Also, this method simultaneously leads to the extraction, concentration, and initial purification of the samples and therefore improves the efficiency ultrafiltration and HPLC. Thus, we introduce this method for the isolation of active proteins and peptides from various complex biological samples, including fish epidermal mucus.