جستجوی مقالات مرتبط با کلیدواژه "histochemistry" در نشریات گروه "شیلات"

The current study investigated histological development and histochemistry of digestive accessory glands of Vimba bream Vimba vimba from hatching through 60th day after hatching (DAH). The samples were randomly taken from day 1 (hatching) until the full absorption of the yolk sac at day 7 and the day after that, then with 2, 5, and 10 days intervals until 60 DAH. The development of the digestive accessory glands was followed histologically using light microscopy after Haematoxylin-Eosin and Periodic-Acid Schiff (PAS) staining. A primordial liver was observed at 1 DAH in the ventral region of the yolk sac, pancreas at 2 DAH, gall bladder and bile duct were developed at 4-5 DAH. At the same time when larvae were growing, digestive accessory glands continued differentiation and, at 5 DAH, hepatocytes of liver with eosinophilic cytoplasm and basophilic granules showed regular shape around sinusoids. Coinciding with the liver development, sinusoids developed as well and hepatic cells increased in size. Also, at this stage, eosinophilic zymogen granules of exocrine portion of pancreas were observed. Secretory activity of digestive accessory glands began with the emergence of PAS positive cells followed by secretion of neutral mucopolysaccharides and glycogen compounds in liver cells of larvae at 4 DAH and pancreatic cells of larvae at 4-7 DAH. The results of this study can be effective in identifying growth limiting factors in breeding larvae, reducing casualties during exogenous feeding, and even developing a proper diet for its digestive system.

The histological analysis, disposition and histochemical localization of tryptophan were investigated in the pancreas to compare the cellular organization and histochemical characterization in the pancreas of Labeo rohita (Hamilton, 1822), Mystus vittatus (Bloch, 1790) and Notopterus notopterus (Pallas, 1769) having different feeding habits. Histological analysis demonstrated that the exocrine pancreatic tissues were dispersed within the hepatic parenchyma and spleen in L. rohita. Thin septa of connective tissue separated parenchyma of liver and also the spleen from exocrine pancreatic cells. However, in M. vittatus, the discrete pancreatic tissue formed distinct oval or elongated acini interspersed with small area of islet of Langerhans and blood vessels. In N. notopterus, the rhomboidal acinar cells of discrete pancreatic tissue intercalated with comparatively clear and large islet of Langerhans. The exocrine acinar cells in all the three species were provided with prominent nuclei and densely packed zymogen granules. Histochemical localization revealed that the zymogen granules of exocrine acinar cells of all species exhibited varied intensities of tryptophan reaction, the precursor of various pancreatic enzymes which may be related to the food and feeding habits of the fishes under study.