جستجوی مقالات مرتبط با کلیدواژه « بیوشیمی خون » در نشریات گروه « شیلات »

To investigate the effect of oral administration of Moringa leaf powder, 360 rainbow trout (35.5 ± 0.5 g) were randomly divided into 12 tanks. The fish were fed with Moringa leaf powder levels of 0(control), 1, 2.5 and 5% for eight weeks. After the challenge test with Aeromonas hydrophila bacteria, the activity of alanine transaminase and aspartate aminotransferase enzymes in the 2.5% treatment significantly decreased compared to the control treatment (P<0.05). The optimal amount of Moringa leaf powder inclusion in the diet for aspartate aminotransferase enzyme based on polynomial regression analysis was 1.97% and 3.33% respectively for the periods before and after the challenge with bacteria. There was no significant change in the amount of alkaline phosphatase enzyme, the number of red blood cells, hemoglobin and hematocrit between all treatments compared to the control treatment in both periods before and after the challenge with bacteria (P<0.05). The amount of glucose showed a significant decrease in all treatments, especially in the 2.5% treatment compared to the control treatment in both stages (P<0.05). Regression analysis showed the optimal value of Moringa for this factor, 3.40% and 2.77%, respectively, before and after the challenge with bacteria. According to the results, moringa leaf powder at 2.5% can help reduce stress and possibly improve the general health of this fish. The appropriate dose was obtained from 1.97% to 3.4% based on polynomial regression model for serum biochemical parameters such as glucose and aspartate aminotransferase enzyme.

Heavy metals can enter to the aquatic animal's body through dietary or water sources and exert their harmful effects. This study was conducted to compare the toxicity of lead in common carp via diet and water borne conditions. Total number of 135 fish (average weight of 50±10 g) was divided into three groups (each with triplicate) including control, water borne (1 mg/L) and diet borne (1 mg/kg dry diet) lead. Experiment was executed during 21 days and sampling was done at 7, 14 and 21 days after trial initiation. Blood biochemical factors including total protein, albumin, globulin, creatinine, glucose, cholesterol, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured in mentioned sampling times. Results showed that lead exposure led to negative effects on blood biochemistry in both groups, but in the case of glucose, LDH and ALP, significant changes were observed between diet and waterborne lead. It may conclud that the diet borne had more toxic effects than waterborne lead and therefore, lead had more potential toxicity through diet in common carp.

This study aimed to determine the fat requirement and effect of dietary fat levels on growth and body composition indices of hybrid Asp (Leuciscus aspius ♀) × Caspian Kutum (Rutilus frisii ♂). For this purpose, 252 fish with an average weight of 29.3 ± 5.8 g were introduced in a completely randomized design with six treatments each with triplicate groups. After two weeks of adaptation, fish were fed by 6 isonitrogenic diets (40% crude protein) with different fat levels including 7, 10, 13, 16, 19 and 22%, 3 times daily according to satiation for 56 days. Some growth indices including final weight, body weight  increase, specific growth  rate, condition factor, feed conversion ratio, protein efficiency ratio, hepatosomatic index and visceral index were considered for this study. The results showed significant differences in various levels of dietary fat in feed conversion ratio and protein efficiency ratio (P<0.05). There was no significant difference on body composition, total protein, total lipid, cholesterol and triglyceride (P>0.05). From the results of the present study, it can be suggest that increasing dietary fat levels up to 16% for better growth performance of this fish.

8-week feeding trial was conducted for juvenile rainbow trout (Oncorhynchus mykiss) to determine the optimal amount of Tenebrio molitor meal (TM) that could replace fishmeal in feeds without reducing growth performance, hematological indices and non-specific immune responses. Five isonitrogenous and isolipidic diets were formulated with TM replacing 0% (Control), 25% (TM25), 50% (TM50), 75% (TM75) and 100% (TM100) fishmeal respectively. Each diet was randomly divided into triplicate groups with 20 juveniles (initial weight 10±0.5 g). At the end of the experiment, the mean of the final weight of the fish fed diets with control (41.7±0.7) and diet containing 25% TM powder (43.1±2.1) was significantly higher than other treatments (p<0.05). The lowest feed conversion ratio was observed in control (0.76±0.1) which had a significant difference compared to TM75 (0.93±0.06) (p<0.05). Weight gain (WG) was affected by dietary TM inclusion and decreased with increasing TM. Red blood cells was not affected by treatment, while hemoglobin content and hematocrit decreased with increasing TM inclusion levels (p<0.05). Plasma complement activity, the IgM level and cortisol were not affected by dietary TM. Dietary treatments had no significant effects on plasma glucose and triglyceride of fish compared to the control (p<0.05). Plasma total protein, albumin and globulin were significantly affected by dietary TM (p<0.05). Plasma cortisol levels and alternative complement (ACH50) pathway activity did not show any significant difference among treatments (p>0.05). According to a broken-line model, the recommended substituted ratio of TM for fish meal was estimated to be 25% for rainbow trout obtaining maximum weight gain.