جستجوی مقالات مرتبط با کلیدواژه « همولنف » در نشریات گروه « شیلات »

In this study, the effects of powder and leaf extract of Moringa oleifera on hemolymph factors of Litopenaeus vannamei in response to salinity stress were evaluated. After 8 weeks of feeding with powder (25, 50 and 100g / kg) and extract (0.25, 0.5 and 0.1 g/kg) leaves of Moringa and control treatment without Moringa, all treatments were exposed to short-term salinity stress (5 and 55 ppt). There was a statistically significant difference in total and differential count of homocytes in different dietary treatments in all three normal conditions and under salinity stress of 5 and 55 pp (p<0.05). Under normal conditions and high salinity, shrimps fed with Moringa leaf extract showed higher number of homocytes than other treatments. In stress-free conditions, the number of semi-granular cells in powder and extract treatments was higher than the control group. At 5 ppt, Moringa powder treatments had higher semi-granular and at 55 ppt salinity, the leaf powder group (100 g/kg) and the leaf extract (0.25 g/kg) showed the highest values, respectively. Overall, the results of this study showed the positive effects of using Moringa leaf powder and extract on innate immune system function and exposure to salinity stress in shrimp

In this study, the effect of different levels of microplastics and copper, alone and in combination, on the number of total hemocytes and different hemocytes in freshwater crayfish (Astacus leptodactylus) was conducted. For this purpose, 378 crayfish with an average length of 10.64±0.76 cm and a weight of 41.12±5.15 grams were divided into 27 aquaria. The exposure period was 28 days, the number of total hemocytes and different hemocytes were checked at the end of the experiment, and hemolymph samples were taken from the crayfish. The results showed that the number of hyalinocytes in the samples exposed to 1 mg/L polyethylene microplastic and 1 mg/L copper sulfate treatment was less than in the control group. The number of granulocytes increased in the treatment of 1 mg/L of polyethylene microplastic and 1 mg/L of microplastic combined with 0.5 mg/L of copper sulfate compared to the control group, and for semi-granulocyte cells, 1 mg/L of fine plastic combined with 0.5 mg/L of copper sulfate significantly decreased and the treatment with 1 mg/L of copper sulfate also had a significant increase compared to the control group (P<0.05). Also, the number of total hemocytes in the treatments of 0.5 mg/L of microplastic combined with 1 mg/L of copper sulfate and the treatment of 1 mg/L of microplastic combined with 1 mg/L of copper sulfate decreased significantly compared to the control treatment (P<0.05), finally it can be concluded that polyethylene microplastic and copper metal alone and together can be among the polluting and damaging factors to the immune system in freshwater crayfish.

This study was designed to evaluate the haemolymph immunological and antioxidant parameters in Penaeus vannamei fed on Sargassum ilicifolium hot-water extract (SHWE) supplemented diets. Three experimental groups including 0.25, 0.5, and 1% SHWE, and one control (without SHWE inclusion), each with three replicates, were applied. A totsl of 120 shrimps (4.67±0.08 g) were randomly distributed into 12 experimental tanks. At the end of a 66-day feeding trial, haemolymph immunological and antioxidant parameters were measured. The results indicated that alkaline phosphatase increased significantly in 0.25% SHWE fed treatment (p < /em><0.05). Glutathione peroxidase significantly increased in all the treatments fed the SHWE supplemented diets (p < /em><0.05). However, the levels of glutathione significantly increased in the groups fed by 0.25 and 0.5% SHWE supplemented diets (p < /em><0.05). The levels of glutathione s-transferase, superoxide dismutase, and catalase significantly increased in the treatment fed 0.5% SHWE supplemented diet (p < /em><0.05), whereas the total antioxidant capacity levels remained unchanged among the treatments (p < /em>>0.05). Both acid phosphatase and phenoloxidase did not influenced by dietary treatments (p < /em>>0.05). The levels of lysozyme were significantly lower in the treatment fed 0.25% SHWE supplementary (p < /em><0.05), whereas this experimental group did not show any difference with the others (p < /em>>0.05). The numbers of THC, GC, SGC, and HC significantly increased in the treatment fed 0.5% SHWE supplemented diet (p < /em><0.05). However, the fish fed 0.25% SHWE supplemented diet exhibited significantly higher THC and SGC levels, compared to the control group (P>0.05). The present results indicate that SHWE can be applied practically via a dietary route to boost the haemolymph antioxidant status as well as the cellular immunity of Pacific white shrimp.

In this study, the effect of experimental diets containing different levels of carbohydrate and niacin on growth indices, carcass composition and heamolymph biochemical parameters of juvenile whiteleg shrimp (Litopenaeus vannamei) were investigated for 45 days. Shrimp with mean initial weight of 5±0.33 g were reared in 300 liter tanks with 8 treatments. Feeding was performed 4 times a day using experimental diets containing two levels of carbohydrate 15 and 30% and three levels of dietary niacin 100, 150 and 200 mg kg-1with two controls. At the end of the period, growth indices, carcass composition and biochemical indices of heamolymph of shrimps were assessed. The best growth performance consisted of the highest final weight (14.05±0.5 g), final length (13.38 ± 0.05 cm) and specific growth rate (2.23±0.07) were in treatment contained 15% carbohydrate and 100 mg niacin kg-1 diet (p<0.05). Also, the highest glucose (53.66±0.88 mg dl-1), HDL (27±0.57 mg dl-1) and total protein values (10.9 ± 0.17 mg dl-1) were in  treatment contained 15% carbohydrate and 100 mg niacin kg-1 diet (p<0.05). On the other hand, the highest protein content of carcass was observed in treatments contained 15% carbohydrate and 100-150 mg niacin kg-1 diet (p<0.05). Also, fat of carcass was reduced by decreasing dietary carbohydrate (p<0.05). Therefore, the results showed that low carbohydrate level with 100 mg niacin per kg of diet had beneficial effects on growth, carcass composition and heamolymph of juvenile whiteleg shrimp.

The purpose of this study was to find the role of 17 beta-estradiol on metabolic parameters and steroid hormones of Litopenaeus vannamei hemolymph . Shrimp with an average weight of 40.18±2.46 g received 0, 20, 40 and 60 mg/kg levels (E0, E20, E40 and E60) in the diet for 25 days. After adaptation to the rearing conditions, 80 shrimps were placed in 4 treatments (each with 2 replications). The results showed no significant changes in the majority of the parameters analyzed, except for the Haemocyanin and protein in the plasma (P>0.05). The highest protein, cholesterol and glucose levels in E40 treatment were 7.22±0.30, 97.65±8.70 and 80.78±4.35 mg/dL, respectively. There was no significant difference in the amount of steroid hormones between treatments with other treatments (except E40 treatment) (P>0.05). Based on the results, it can be reported that adding 40 mg of hormone to broodstock commercial feed improves hormonal activities of L. vannamei.