جستجوی مقالات مرتبط با کلیدواژه « Complement » در نشریات گروه « شیلات »

Little is known about complement system as a component of innate immunity of the ectothermic vertebrates such as crocodiles. Serum complement is valuable tool in determining the health status of turtles. In this research effect of concentration, volume and temperature on alternative complement pathway activity of Mugger crocodiles (Crocodylus palustris) using standard haemolytic assays was studied. For this purpose, Crocodylus palustris serum complement hemolysis in different concentrations, volumes and temperatures was measured. Results indicated significant relation between relation measured hemolysis and concentration, volume and temperature (p<0.05). The measure of hemolysis in various concentrations (25%, 50% and 100%), volumes (10, 20, 30 µL) and temperatures (5-35°C) were significantly increased (p<0.05). The results show that alternative complement pathway activity of crocodiles might affect concentration, volume and temperature that it caused increased non-specific immunity and the resistance to the outbreak of diseases.

The present study was designed to evaluate the effect of acetaminophen on hematologic and blood biochemistry of rainbow trout in a 4-day period. To that end, total 300 fingerlings with an average body weight of 12.5±2.5 g were randomly allocated into 15 polyethylene tanks (with 100L volume). Fingerlings were exposed to five concentrations of acetaminophen, namely, 0, 0.01, 0.1, 1. 10 mg/L for 4 days. Hematologic and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) exposed to Acetaminophen were measured. According to the results, the number of white blood cells was significantly different compared to the control group and other treatments at concentration of 10 mg/l. The number of red blood cells, hemoglobin, mean hemoglobin volume (MCV) and hematocrit were higher in the control group and And the lowest amount was observed at a concentration of 10 mg/l and There was a significant difference between these parameters in different treatments. There wasn´t significant difference in mean corpuscular hemoglobin concentration (MCH) between experimental treatments. There was a significant difference in serum lysozyme enzyme and complement (ACH50) activity in fingerling exposed to acetaminophen. There was a significant difference in cortisol and glucose levels in different treatments so that the most amount of cortisol and glucose showed in the control group and the less amount was observed in treatment 5.

In the present study, the effect of the intraperitoneal injection of Carthamus tinctorius extract on non-specific immune system and some serological of Oncorhynchus mykiss has been investigated. To this end, the fish with average weight of 100±10 gr were intraperitoneally injected by different levels of safflower extract. In this regard, a negative control group (no injection), a positive control group (injection with 0.2 ml physiologic serum) and three treatment groups received injections of C. tinctorius extract with the concentration of 50, 100 and 200 (mg/kg weight in the volume of 0.2 ml) respectively. Blood sampling were taken On the third, seventh and tenth days after injection, and total serum protein, total immunoglobulin, lysozyme activity, complement and blood glucose levels determined. The results showed that safflower extract injection led to a significant decrease in the number of white blood cells on the third, seventh and tenth days after injection (sig< 0/05). Moreover, blood glucose, lysozyme activity, total immunoglobulin and total protein levels significantly decreased. There was no significant change in complement activity among all groups. Considering this results, the use of safflower extract in rainbow trout can reduce and non-specific immune system activities.