جستجوی مقالات مرتبط با کلیدواژه « Antioxidant Activity » در نشریات گروه « صنایع غذایی »

In this study, anthocyanin and phenolic compounds were extracted from pomegranate peel powder with supercritical CO2 and Lepidium perfoliatumseed gum and maltodextrin were used as the nanoencapsulation wall. The nanoencapsulation extract was dried with a freeze dryer. Color stability and antioxidant activity of nanoencapsulation extract were evaluated. The levels of anthocyanin and phenolic compounds were evaluated by differential pH and Folin-Ciocalteu method, respectively. Particle size, nanoencapsulation efficiency of anthocyanin,nanoencapsulation phenol, color stabilityat different temperatures and pH as well as antioxidant activity were evaluated by DPPH. The amount of anthocyanin and phenol extracted in this method was 3.943±0.133 mg/g of pomegranate peel powder and 504.521±2.537 mg/g of gallic acid per 100 g, respectively. Nanoencapsulation efficiency was higher for anthocyanin than phenols. The release of anthocyanin at different pHs was different, so that anthocyanin behaved differently at different pHs. Polymerization and color increased with increasing temperature. The antioxidant activity of the fine-grained extract by DPPH method increased with increasing concentration and its IC50 was equivalent to 1.092± 0.024 m /ml.

This study aimed to investigate the chemical composition, antioxidant properties, and antimicrobial activity of Tanacetum balsamita L. essential oil. The essential oil was extracted using the water distillation method, and then the total phenolic content (using the Folin-Ciocalteu method), total flavonoid content (using the aluminium chloride colorimetric method), antioxidant activity (using DPPH and ABTS free radical scavenging methods), and antimicrobial activity (using disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration methods) were evaluated. The total phenolic and flavonoid contents of the essential oil were found to be 29.50 mg gallic acid equivalent per gram and 14.90 mg quercetin equivalent per gram, respectively. The essential oil exhibited significant antioxidant activity, with the ability to scavenge 50.40% and 53.55% of free radicals DPPH and ABTS, respectively. According to the results of disc diffusion agar method, Bacillus subtilis and Salmonella typhi were the most sensitive and resistant bacterial strains, respectively, with zone of inhibition diameters of 16 mm and 10.50 mm. The zone of inhibition diameters for these bacteria in the well diffusion agar method were obtained as 16.30 mm and 10.80 mm, respectively. The minimum inhibitory concentration and minimum bactericidal concentration for B. subtilis were determined as 4 mg/mL and 32 mg/mL, respectively, and for S. typhi, they were 64 mg/mL and greater than 512 mg/mL, respectively. According to the results, T. balsamita essential oil can be used as a natural antioxidant and antimicrobial agent in food products.

Kefir is a dairy that contains lactic acid, carbon dioxide, and other volatile aromatics traditionally produced by the activity of kefir dunkers (Gaware et al., 2011). One of the constituent components of kefir grain are mucoid substances that are produced by the microbial flora of this grain, and exopolysaccharide called kefiran is one of them. Radhouani et al. (2018) showed that kefir is a suitable material for use in the field of biomaterials production, film and tissue formation. The most prominent biodegradable biopolymers used in food packaging from the group of biopolymers produced by microorganisms are bacterial cellulose and kefir (Rhim et al., 2013 and Sabatino et al., 2020). Kefiran is able to produce transparent films with relatively good mechanical resistance. Recently, the antimicrobial effect of postbiotics has attracted the attention of researchers. Postbiotics are actually a byproduct of the fermentation process done by probiotics (Moradi et al., 2019). Silver nanoparticle is one of the nanomaterials that has been mentioned in many studies for its antimicrobial effect as well as the effect of improving the mechanical and barrier properties of biodegradable films (Ajitha et al., 2021; Blosi et al., 2021; Li et al., 2023). One of the new methods in the production of biopolymer films that has recently attracted the attention of researchers is the electrospinning method. Electrospinning is a process in which continuous polymer fibers with diameters in the sub-micrometer range are produced through the action of a high-voltage electric field applied to a solution.

To cultivate kefir seeds, kefir grains were first cultured in fresh cow's milk for activation and growth. In order to increase the activation speed, greenhouse was used at a temperature of 27°C. To extract kefir from kefir grains, kefir grains were mixed with distilled water at a ratio of 1:10 at a temperature of 81°C and then the resulting mixture was centrifuged at a temperature of 20°C for 20 minutes. Then, kefirs using 96% ethanol were centrifuged at a temperature of 4°C for 20 minutes, and the obtained precipitates were mixed with distilled water at a temperature of 80°C in a ratio of 1:5. This washing step was repeated twice until a white deposit of kefir was obtained and then, was dried.In order to extract the postbiotic extract, first, 5 g of kefir seeds were inoculated in the milk culture medium and placed in a greenhouse at 35°C for 48 hours. Then the culture medium was centrifuged and the supernatant was filtered with a needle filter. In this study, a concentration of 4% kefir was used to produce electrospun nanofibers. In this way, 0.04 g of kefir was added to 10 ml of postbiotic extract and electrospuned (Ethnaashri et al. 2024). To prepare the next treatments, three levels of silver nanoparticles (1, 2.5, and 4% by weight of kefir) were added to the previous solution and treated with an ultrasonic probe for 5 minutes (40 kHz, 100W).Also, in order to study the properties of the nanofibers, FTIR, XRD, DSC and SEM analysis were performed and the contact angle, antioxidant activity and antimicrobial activity of them were studied.

This research was designed and implemented with the aim of developing an active electrospun nanofiber substrate for use in food preservation. Kefiran nanofibers containing kefir postbiotics in constant concentration and silver nanoparticles in different concentrations were prepared and their structural, morphological and functional properties were evaluated. Comparison of FTIR spectra of nanofibers containing nanosilver shows that the addition of nanoparticles had no effect on the chemical structure of nanofibers. Due to the small amount of nanosilver in the formulation of nanofibers, the peak related to the nanoparticles itself was not observed. The lack of change in the spectrum of kefir chemical groups due to the addition of nanosilver means that the nanoparticles are physically bound in the polymer network of kefir nanofibers. SEM images show tha nanofibers had a uniform cylindrical shape with a smooth surface and no knots. The average diameter of kefiran nanofibers without nanosilver was about 245 nm. Also, by increasing the concentration of silver nanoparticles to 2.5%, the size of nanoparticle clusters on the surface of nanofibers increased and the average diameter of kefir nanofibers decreased to 205 nm. XRD analisys of kefiran nanofibers showed a sharp peak at the angle of 2θ=44.5°. The results indicate the semi-crystalline nature of kefir nanofibers. With the increase of silver nanoparticles, there is no change in the intensity and location of the main peak at the angle of 44.5°, but a new peak is created at the angle of 2θ = 1.38° and the highest intensity is observed in the sample containing 4% nanosilver. The control nanofibers of kefir containing postbiotics showed three ranges of changes in the DSC test. The main melting peak of this nanofiber was observed at 73°C. By adding silver nanoparticles, the thermal properties of kefir nanofibers were weakened. The results show that silver nanoparticles affected the thermal properties of kefir and reduced its resistance to temperature. The contact angle of nanofibers by adding of silver nanoparticles at a concentration of 1%, shwed no significant change (p>0.05). But when the amount of silver nanoparticles reached 2.5% and 4%, the contact angle decreased significantly (p<0.05) and in fact the hydrophilic property of nanofibers increased. The antibacterial activity of electrospun kefir nanofibers containing postbiotics and nanosilver was investigated against E. coli and S. aureus. By increasing the amount of silver nanoparticles, the antimicrobial property increased and the sample containing 4% nanosilver showed the highest antibacterial activity against both bacteria. In this research, kefir nanofibers containing postbiotic showed an antioxidant activity of 5.65%, which is due to the low amount of postbiotic in the nanofiber formulation. By increasing the concentration of silver nanoparticles, the antioxidant activity increased significantly, and the highest inhibitory activity was observed at a concentration of 4% with a power of 59.21%.

The results of the FTIR test proved the physical connection and the lack of chemical bonding between silver nanoparticles and nanofibers. Good and uniform surface morphology was observed in kefir nanofibers in SEM test, but silver nanoparticles were observed in aggregated form. The XRD test showed no effect of nanosilver on the structure of kefiran nanofibers, but in the DSC test, the weakening of the thermal properties of kefiran nanofibers was reported with an increase in the concentration of nanosilver. The control nanofibers of kefirs that had only postbiotics also had antioxidant and antimicrobial properties, but these functional properties were enhanced by increasing the concentration of silver nanoparticles, and nanofibers containing 4% nanosilver showed the best antimicrobial and antioxidant properties. In general, the results of this study showed that kefir nanofibers containing postbiotic kefir and silver nanoparticles have favorable physical and functional characteristics and can be used as active food packaging and can be used for the purposes of increasing the shelf life of food and preventing microbial and oxidative spoilage.

Seeds and nuts have received increasing attention due to their nutritional value and the high therapeutic properties of their bioactive compounds. Most of the seeds are used as nuts, and some of them are considered agricultural waste. Pumpkin seeds have a high content of protein (30–40% in terms of dry matter). Proteins are among the vital health-giving components that provide nitrogen, essential amino acids and energy necessary for normal cells. Pumpkin seeds are a good source of amino acids such as valine, histidine, isoleucine, leucine, threonine and methionine. Protein hydrolysate is a mixture of peptides and amino acids that can show antioxidant, antimicrobial, anticancer, antidiabetic and antihypertensive properties. During hydrolysis, proteins are broken into small peptides and amino acids. Since enzymatic hydrolysis is performed in relatively mild conditions and no amino acid damage occurs, this type of hydrolysis is preferred over acid and alkaline hydrolysis. Hydrolysates obtained from pumpkin seed protein have bioactive properties, especially antioxidant activity. Pretreatment of proteins before enzymatic hydrolysis acts to improve the release of bioactive peptides from different proteins. Pretreatment can facilitate the unfolding the structure of proteins and thus increase the access of enzymes to peptide bonds. The main properties of microwaves usually show three characteristics: penetration, reflection and absorption. Microwave assisted enzymatic hydrolysis can shorten the time  and improve the speed of the reaction. The purpose of this research was to investigate the antioxidant activity of pumpkin seed protein hydrolysates (Cucurbita maxima L.) by alcalase enzyme in two conditions: without pretreatment and using microwave pretreatment.

In this study, Pumpkin (Cucurbita maxima L.) was purchased from the local market of Astane Ashrafieh in Gilan province. The seeds were scooped manuallyand then dried in an oven at 50°C for 72 hours. After the production of protein concentrate from pumpkin seeds, the chemical properties of the concentrate, such as the amount of fat, protein, ash and moisture, were measured. The isolated pumpkin seed solution was exposed to microwave energy with a power of 450-900 watts for 30–90 seconds and was used as a substrate solution in enzymatic hydrolysis experiments. It should be noted that after measuring the total antioxidantactivityr for different powers and times of microwave pretreatment, the power of 600 watts for 60 seconds was selected  and applied before enzymatic hydrolysis. Enzymatic hydrolysis was done by alcalase enzyme with a concentration of 0.5 to 2.5% compared to the protein substrate during 20 to 190 minutes, and the optimum temperature and pH of alcalase were determined in order to produce hydrolysates with antioxidant activity. Antioxidant activity was measured by using DPPH free radical inhibition, total antioxidant activity and iron chelation activity methods.

Bioactive peptides produced by the enzymatic hydrolysis of proteins have significant antioxidant properties. Pumpkin seeds can be used as a rich source of nutrients and bioactive compounds in various food industries. The results showed that the maximum amount of antioxidant activity without pre-treatment was achieved in 165 minutes with a 2.2% ratio of E/S by using DPPH free radical scavenging activity (40.5%), total antioxidant power (0.79), and iron chelation activity (96.2%) methods. By using microwave pre-treatment, the maximum amount of antioxidant activity was achieved in a shorter time and with less enzyme (105 minutes and E/S ratio 1.5%) using DPPH free radical scavenging (52%), total antioxidant power (0.711), and iron chelation activity (93%). Therefore, it can be concluded that using microwave assisted enzymatic hydrolysis , in addition to achieving hydrolysates with proper antioxidant activity, is a suitable method to save time and reduce enzyme concentrations used in enzymatic hydrolysis.

Herbal bioactive compounds are secondary metabolites of plants that are produced in response to environmental stress and to protect the plant against harsh conditions. These compounds have both health effects on humans and also have preservative effects in food products. The conditions for extracting active compounds have a significant effect on their functional activities, and conventional and new methods have been used to extract bioactive compounds from plants. The aim of this study was to compare the extraction methods of bioactive compounds of Persian Golnar extract (PGE). In this research three methods of ohmic (temperatures of 45 °C and 60 °C for 40 and 60 min), ultrasonic (temperatures of 40 °C, 50 °C and 60 °C for 20 and 40 min) and perculation (for 24, 48 and 72 h) were used to prepare PGE, and the best extract was selected based on the extraction yield, total phenol content (TPC), total anthocyanin content (TAC) and antioxidant activity (DPPH radical scavenging). The results showed that there was no significant difference between the extraction yields of all three methods (p>0.05). The ohmic method had the same TPC as the ultrasonic method, but showed a higher TAC and antioxidant activity and was chosen as the best method. The optimal treatment in this stage included ohmic method, 60 °C and 40 min, and the extract obtained under these conditions contained 109.74 mg GAE/g of TPC, 373.26 mg/g of TAC and 81.11% antioxidant activity.

The use of safe ingredients to preserve food is steadily increasing. The high time and cost of production and approval of synthetic food additives and the reduction of public acceptance of these compounds have caused serious problems in their utilization. Excessive use of synthetic preservatives, which some of them are suspected to be toxic, has completely eliminated these additives and led to the use of natural alternatives to preserve or extend the shelf life of food products. Many plant-based bioactive compounds are good alternatives to synthetic antimicrobial and antioxidant supplements. Plant extracts have significant biological activity including antioxidant, antibacterial, and antifungal properties, which has increased their use in food products. In addition, plant-derived antimicrobial compounds have been considered in the pharmaceutical industry to control microbial pathogens. Natural antioxidant and antimicrobial compounds are receiving a lot of research and industrial attention in food preservation technologies. In the last 2 decades, the use of herbal medicines rich in bioactive molecules (including polyphenols, carotenoids and flavonoids) with medicinal and health effects such as delaying the onset of some diseases such as cardiovascular disorders, diabetes, and cancer have increased.The plant Prosopis farcta grown in arid and semi-arid regions. In Iran, it is found in the southern regions of the country. In traditional medicine, this plant is used to prevent hyperlipidemia and hyperglycemia, to treat hemorrhoids, intestinal diseases and diarrhea, and leprosy, and to reduce abortion. In addition, antimicrobial and antioxidant properties of various species of Prosopis have been reported. Accordingly, in this study, after examining the of total phenols and flavonoids concentrations, the antioxidant and antimicrobial properties of ethanolic extract of Prosopis farcta were determined. 

The ethanolic extract of P. farcta was obtained maceration method. Total phenol content (by Folin-Ciocalteu reagent method), total flavonoid content (by aluminum chloride method), antioxidant activity (by DPPH and ABTS free radical scavenging and beta-carotene bleaching methods), and antimicrobial effect against Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis (by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration) of the extract were evaluated. 

farcta ethanolic extract showed high phenol content (145.58 ± 1.30 mg GAE/g), while its total flavonoid content was 72.37 ± 1.48 mg QE/g. Antioxidant activity of ethanolic extract of melon root using different methods of DPPH and ABTS free radical scavenging and beta-carotene bleaching inhibition were 62.60, 71.82 and 54.50%, respectively. Antibacterial activity of P. farcta ethanolic extract against Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis according to disk diffusion agar and well diffusion agar methods showed that the antimicrobial activity of the extract was concentration dependent and Shigella dysentery and Staphylococcus aureus were the most resistant and sensitive bacterial strains to the extract respectively. The minimum inhibitory concentrations of ethanolic extract of P. farcta root for Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis were 8, 8, 4 and 4 mg/ml, respectively; while the minimum bactericidal concentrations for these bacteria were 128, 256, 32 and 64 mg/ml, respectively. 

In the present study, ethanolic extract obtained from the roots of P. farcta was identified as a rich source of phenolic and flavonoid compounds. The ethanolic extract showed effective antimicrobial and antioxidant properties. The results greatly indicated the promising effect of P. farcta root extract against Gram-positive and Gram-negative bacterial species. As the microbial resistance is constantly increasing, ethanolic extract of P. farcta root can be considered as a suitable complementary option to tackle this problem. In addition, the identification of individual components of P. farcta ethanolic extract and their biological functions or their combination with common antioxidant and antimicrobial agents could be the subject of future research.

Yogurt, a widely consumed dairy producton a global scale, is highly regarded for its outstanding nutritional composition, gastrointestinal advantages, prolonged storage capability, rapid digestibility and assimilation rates, economic importance, and therapeutic properties.  And It is shown in this research illustrates that yogurt with an increased quantity of alfalfa extract elevates the levels of phenolic compounds and antioxidant capabilities. In this study, alfalfa extract was incorporated into yogurt formulation at varying concentrations of 2, 4, 6, and 8 percent by weight. Following a storage period of 20 days, the assessment of antioxidant activity was conducted by measuring DPPH radical inhibition. Moreover, an analysis of phenolic compounds and an evaluation of yogurt quality attributes including pH, acidity, water content, and viscosity were carried out. Subsequently, a factorial experiment design was employed, utilizing a completely randomized design with three replications for statistical purposes. Statistical analysis involved the comparison of means at a significance level of 1% using SAS software version 9. The results showed that the presence of alfalfa extract led to an augmentation in the levels of water-soluble phenolic compounds as well as the antioxidant capacity of yogurt. So that, at the conclusion of a 10-day period, the highest antioxidant activity was associated with yogurt formulations incorporating 6% and 8% alfalfa extract. Furthermore, the yogurt sample containing 8% extract exhibited the lowest water content, a statistically significant variance compared to the remaining samples (p≤0.05). It should be noted that Considering the impact of dairy products on health and the need to increase per capita consumption of dairy products, more attention should be paid to the production of products that have more health benefits and high rheological properties. Hence, within this study, it was demonstrated that the inclusion of alfalfa extract resulted in an augmentation of the samples' antioxidant characteristics.

Nowadays, with the increase in diseases and consumers’ preference for organic foods, the use of natural plant compounds to improve and increase their shelf life is on the rise. In addition, the use of medicinal plants, which can have therapeutic properties, has attracted attention. Black seed oil has attracted a lot of attention in the past due to its unique therapeutic properties. The aim of this study was to investigate the antioxidant activity and evaluate the antimicrobial activity of black seed oil against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The amount of phenolic compounds in black seed oil was measured by Folin-Ciocalteu. The antioxidant activity was determined by DPPH and ABTS radical scavenging methods. The antimicrobial effect of Nigella sativa oil was investigated according to well diffusion  agar, disk diffusion agar, interaction, minimum inhibitory concentration and minimum bactericidal concentration methods.The Nigella sativa oil had 16.25 mg GAE/g phenolic compounds. The antioxidant activity based on DPPH and ABTS radical scavenging were 86.62% and 94.85%, respectively.The Nigella sativa oil showed a significant antimicrobial effect on the tested microorganisms. In the combined mode (interaction) of Nigella sativa oil with chloramphenicol antibiotic, synergistic mode was observed for all bacterial strains. Given the significant antioxidant activity and antimicrobial effect of Nigella sativa, it can be used in food and pharmaceutical industries.

Coffee Mix is one of the most popular instant drinks in the world, which is widely consumed. This product can cause nutritional problems due to the significant amount of sugar in the formulation. On the other hand, due to its beneficial properties, honey and ginger powder can be used in the instant coffee powder formula, to solve the anti-nutritional effects of sugar and to take advantage of the beneficial properties of ginger and honey powder. In this study, the effect of adding honey powder as a substitute for sugar in amounts of 0, 5, 10, 20, 30, and 35% of the consumed sugar and ginger powder in amounts of 0, 0.05, 0.1, 0.2, and 3 0.0 and 0.35% were added to the 3 in 1 instant coffee formulation and the physicochemical properties, phenolic compounds, antioxidant capacity and sensory properties and microbial properties of the coffee mixture were evaluated. The research results showed that adding honey and ginger powder to the instant coffee mixture improves its physicochemical properties and overall acceptability. Sensory tests conducted by the evaluators also determined that the treatment of 20% honey powder and 0.2% ginger and the treatment of 10% honey powder and 0.3% ginger were more favorable samples in terms of overall acceptability. The treatment of 30% honey powder and 0.3% ginger had the highest amount of phenolic compounds and antioxidant capacity. Therefore, the most appropriate treatment in terms of nutritional characteristics was the treatment of 30% honey powder and 0.3% ginger.

The seeds of Cucurbitaceae plants (pumpkin, melon, etc.) are one of the rich sources of protein. Proteins are one of the most important nutritional sources for humans. Enzymatic hydrolysis of pumpkin seed protein by using microwave pretreatment leads to the production of hydrolyzates with bioactive properties, including antioxidant activity. The use of microwave pretreatment causes changes in the 3D structure of proteins, It opens the 3-dimensional structure of the protein and accelerates the access of the enzyme to the peptide bonds. Therefore, the use of microwave pretreatment is a suitable method to save time and enzyme concentration which are used in enzymatic hydrolysis. In this study, The solution of pumpkin seed protein concentrate was exposed to microwave energy with a power of 500-900 W for 30-90 seconds and it was used as a substrate solution in enzymatic hydrolysis experiments. Enzymatic hydrolysis by pancreatin, with the concentration of 0.5 to 2.5% compared to the protein substrate, was performed in the time from 20 to 190 minutes, at the optimum temperature and pH of pancreatin, in order to produce hydrolysates with antioxidant potential. Antioxidant power was measured by using DPPH radical scavenging activity methods, total antioxidant activity (Absorbance at 695 nm) and iron chelating activity. The highest amount of antioxidant activity by using microwave pretreatment was in 105 minutes and the ratio of 1.5% E/S and the optimal conditions that provided by the software to achieve the maximum iron chelating activity (95.5%), DPPH radical scavenging activity (51.5%) and total antioxidant (Absorbance at 695 nm) (0.976), was in 102 minutes and the ratio of 1.5% E/S of which 89.5% corresponded with the obtained results.

One of the critical methods to maintain the stability and functional properties of plant essential oils as a useful source of bioactive compounds against environmental damage is their encapsulation in nanocarrier systems such as nanoliposomes. In this study, nanoliposome containing the citron peel essential oils were prepared without the use of toxic organic solvent and by employing health-giving compounds such as sesame oil in addition to lecithin for the first time in the formulation. The stability of the samples during 30 days of storage at temperatures of 4ºC and -18ºC was determined by investigating the retention amount of phenolic compounds, pH changes, antioxidant and antimicrobial performance. The nanoliposomal samples of essential oils of hydrodistillation and supercritical CO2 of citron peel prepared with different concentrations of lecithin oil had different quantities of pH and phenol retention percentage, and their amount reduced with increasing storage time at both test temperatures. DPPH inhibitory ability and antimicrobial activity of both citron peel essential oils were improved after encapsulation in nanoliposome. But their amount in both storage temperatures decreased with the advancing of time. The nanoliposome of the supercritical fluid essential oil of citron peel respectively with the formulation containing the highest and lowest amount of lecithin oil at the storage temperature of 4ºC showed the best result in this study. Therefore, the citron peel essential oil with encapsulation in the nanoliposome system prepared from lecithin-sesame oil, due to improvement of antioxidant and antimicrobial activity and its higher stability against storage temperature, can be used as an effective natural functional additive in the food industry.

Notice to optimizing the use of medicinal plants by producing soft drinks can be a suitable solution in order to encourage consumers to use food with natural source that have beneficial effects on the health. The purpose of this research is optimizing the extraction process of Hibiscus Tea and providing the best way to preserve the nutritional value of this plant during extraction and applying this extract in diet soft drinks formulation by using the natural sweetener Stevia and evaluation of physico-chemical changes during storage. Thus, extracts of Hibiscus Tea was done in three temperatures of 60, 75 and 90 ° C in the range of 10 to 20 minutes and to select the best extraction temperature and time in Hibiscus Tea samples, terms of PH, antioxidant activity, anthocyanins and phenolic compounds were studied. Then by using extraction of Hibiscus Tea, soda with four different formulations was prepared. To consider the durability of soda, in 90 days, every 30 days lasting soft drink samples in terms of antioxidant activity, anthocyanins and phenolic compounds were evaluated. Finally it was determined that temperature 41.71 ° C for 18.81 minutes is the best temperature and time for extracting Hibiscus Tea, that In this optimal conditions, the antioxidant activity 62.4062%, the amount of anthocyanins 72.394 mg/l and total phenolic compounds 65.2564 mg/100 ml was determined. Also it was detrmined that antioxidant activity, anthocyanins and phenolic compounds at the end of storage time than the first day of manufacturing soft drinks decreased.

The aim of this research was to optimize the formulation of a functional drink based on sugarcane juice using the response surface method (RSM) and in the form of Box Benken design. The ingredients used in the formulation were lemon juice (0.3, 6), turmeric (0.6, 0.3) and mint extract (0, 250, 500) in percentage and their physicochemical properties (pH, acidity, soluble solids, turbidity, color, total phenolic compounds, antioxidant activity) and sensory characteristics were investigated. The results showed that increasing the amount of lemon juice increased acidity, turbidity, L index, b index, phenolic compounds, antioxidant capacity and overall acceptance, but decreased pH, soluble solids and a index. The increase of turmeric caused a slight increase in the amount of soluble solids, b index and total phenolic compounds, while it decreased the antioxidant property and overall acceptance of the samples. With the increase in the amount of mint extract, turbidity, a and b indices, phenolic compounds and overall acceptance increased, but the amount of soluble solids and L index decreased. Finally, the optimization results showed that the sugarcane juice drink containing lemon juice (6%), turmeric (0.2%) and mint extract (418%) had the best physicochemical properties and the highest overall acceptance and can be recommended as a healthy drink in the industry.

The present study aims to shed light on the effects of encapsulation by the nanoemulsion (NE) method of garlic essential oil (GEO) on the oxidative stability of olive oil. To this end, the effect of different GEO percentages on NE droplet size, encapsulation efficiency, antioxidant properties, stability, and turbidity at concentrations of 200, 400, and 600 ppm was evaluated. The results showed that the droplet size of NEs ranged from 50.75 to 220.20 nm. The NEs were added to olive oil, and the oil’s peroxide, thiobarbituric acid, iodine, acid values, antioxidant properties, and sensory properties were measured at 1, 30, 60, and 90 days of storage. According to the findings, from day 30th to 90th, the lowest amount of peroxide, thiobarbituric acid, and acid values, and also the highest antioxidant activity (lowest IC50), were detected in the sample with the highest concentration of NE (600 ppm of garlic essential oil nanoemulsion [GEON] encapsulated with Arabic gum).